A proteomic study to unveil lead toxicity-induced memory impairments invoked by synaptic dysregulation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36518382", "kind": "science-record", "revision": 4, "content_hash": "sha256:ec88e2409c64aa4b4517187f95a7119f7216b85d8ee284ee6444121f004c55a6", "created_at": "2026-11-01T19:01:01Z", "updated_at": "2026-11-01T22:50:50Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "A proteomic study to unveil lead toxicity-induced memory impairments invoked by synaptic dysregulation.", "doi": "10.1016/j.toxrep.2022.07.002", "pmid": "36518382", "authors": ["Nivedha Mohanraj", "Neha S Joshi", "Roshni Poulose", "Rahul R Patil", "Rashmi Santhoshkumar", "Anubhav Kumar", "Girish P Waghmare", "Amit Kumar Saha", "Syeda Zehra Haider", "Yogananda S Markandeya", "Gourav Dey", "Laxmi T Rao", "Periyasamy Govindaraj", "Bhupesh Mehta"], "journal": "Toxicology reports", "year": 2022, "abstract": "Lead (Pb2+), a ubiquitously present heavy metal toxin, has various detrimental effects on memory and cognition. However, the molecular processes affected by Pb2+ causing structural and functional anomalies are still unclear. To explore this, we employed behavioral and proteomic approaches using rat pups exposed to lead acetate through maternal lactation from postnatal day 0 (P0) until weaning. Behavioral results from three-month-old rats clearly emphasized the early life Pb2+ exposure induced impairments in spatial cognition. Further, proteomic analysis of synaptosomal fractions revealed differential alteration of 289 proteins, which shows functional significance in elucidating Pb2+ induced physiological changes. Focusing on the association of Small Ubiquitin-like MOdifier (SUMO), a post-translational modification, with Pb2+ induced cognitive abnormalities, we identified 45 key SUMO target proteins. The significant downregulation of SUMO target proteins such as metabotropic glutamate receptor 3 (GRM3), glutamate receptor isoforms 2 and 3 (GRIA 2 and GRIA3) and flotilin-1 (FLOT1) indicates SUMOylation at the synapses could contribute to and drive Pb2+ induced physiological imbalance. These findings identify SUMOylation as a vital protein modifier with potential roles in hippocampal memory consolidation and regulation of cognition.\nData availbility: The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD034212&#34.", "claims": [{"id": "claim-method", "statement": "For behavioural data, statistical analysis was performed by two-way ANOVA using Origin Pro software. Statistical analysis for the quantified proteins was carried out using the Perseus software platform. Two sample t-test was done, and the p-value ≤0.05 was set as a cut-off for significant proteins.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36518382", "locator": "sections[16] \"Statistical and bioinformatic analysis\" paragraph 2", "quote": "For behavioural data, statistical analysis was performed by two-way ANOVA using Origin Pro software. Statistical analysis for the quantified proteins was carried out using the Perseus software platform. Two sample t-test was done, and the p-value ≤0.05 was set as a cut-off for significant proteins."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36518382"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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