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pmid:36524001
Vemurafenib inhibits immune escape biomarker BCL2A1 by targeting PI3K/AKT signaling pathway to suppress breast cancer.
Yalan Dai · Liqiong Yang · Abass Sakandar · Duoli Zhang · Fukuan Du · Xinyi Zhang · Linglin Zou · Yueshui Zhao · Jigang Wang · Zhenhua Zhang · Xu Wu · Mingxing Li · Xiao Ling · Lei Yu · Lishu Dong · Jing Shen · Zhangang Xiao · Qinglian Wen
Frontiers in oncology · 2022 doi:10.3389/fonc.2022.906197
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Experiment
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Analysis
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Complete file
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Sample information and clinical data on BC were obtained from the TCGA and GTEX databases. Obtaining differentially expressed protein data from cBioportal database. To construct a risk score model by lasso analysis, and nomogram was used to predict score core. GSCA, TIMER and CELLMINER databases were used for immune and drug susceptibility correlation analyses. Cell experiments were verified by MTT, Western blotting, and RT-qPCR.\nResults: We found prognostic models consisting of eleven immune escape related protein-coding genes with ROC curves that performed well in the ontology data (AUC for TCGA is 0.672) and the external data (AUC for GSE20685 is 0.663 and for GES42568 is 0.706). Five core prognostic models are related to survival (EIF4EBP1, BCL2A1, NDRG1, ERRFI1 and BRD4) were summarized, and a nomogram was constructed to validate a C-index of 0.695, which was superior to other prognostic models. Relevant drugs targeting core genes were identified based on drug sensitivity analysis, and found that Vemurafenib downregulates the PI3K-AKT pathway and BCL2A1 protein in BC, as confirmed by external data and cellular assays.\nConclusions: Briefly, our work establishes and validates an 11-immune escape risk model, and five core prognostic factors that are mined deeply from this model, and elucidates in detail that Vemurafenib suppresses breast cancer by targeting the PI3K/AKT signaling pathway to inhibit the immune escape biomarker BCL2A1, confirms the validity of the prognostic model, and provides corresponding targeted agents to guide individualized treatment of BC patients.", "claims": [{"id": "claim-method", "statement": "Univariate Cox analysis was performed on 181 differentially expressed protein coding genes associated with immune escape in BC, and 31 of them were identified to be associated with survival ( Figure 3A ). To evaluate differentially expressed protein-coding genes meaningfully associated with BC prognosis and to obtain a better-fitting model, we used LASSO to downscale the high-dimensional information by adding a constraint to the absolute value of the coefficients and further screening by 10-fold cross-validation. The optimal λ value (λmin=0.025) was obtained from the minimum local likelihood deviation, and 16 genes were found to be significantly associated with prognosis ( Figures 3B, C ).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36524001", "locator": "sections[19] \"An 11-gene prognostic risk model was constructed and validated with external data sets\" paragraph 2", "quote": "Univariate Cox analysis was performed on 181 differentially expressed protein coding genes associated with immune escape in BC, and 31 of them were identified to be associated with survival ( Figure 3A ). To evaluate differentially expressed protein-coding genes meaningfully associated with BC prognosis and to obtain a better-fitting model, we used LASSO to downscale the high-dimensional information by adding a constraint to the absolute value of the coefficients and further screening by 10-fold cross-validation. The optimal λ value (λmin=0.025) was obtained from the minimum local likelihood deviation, and 16 genes were found to be significantly associated with prognosis ( Figures 3B, C )."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/ Supplementary Material . 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Further inquiries can be directed to the corresponding author/s."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We found prognostic models consisting of eleven immune escape related protein-coding genes with ROC curves that performed well in the ontology data (AUC for TCGA is 0.672) and the external data (AUC for GSE20685 is 0.663 and for GES42568 is 0.706).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36524001", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "We found prognostic models consisting of eleven immune escape related protein-coding genes with ROC curves that performed well in the ontology data (AUC for TCGA is 0.672) and the external data (AUC for GSE20685 is 0.663 and for GES42568 is 0.706)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "We found prognostic models consisting of eleven immune escape related protein-coding genes with ROC curves that performed well in the ontology data (AUC for TCGA is 0.672) and the external data (AUC for GSE20685 is 0.663 and for GES42568 is 0.706).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36524001"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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"GSM2528353", "GSM2528354", "GSM2528355", "GSM2528356", "GSM2528357", "GSM2528358", "GSM2528359", "GSM2528360", "GSM2528361", "GSM2528362", "GSM2528363", "GSM2528364", "GSM2528365", "GSM2528366", "GSM2528367", "GSM2528368", "GSM2528369", "GSM2528370", "GSM2528371", "GSM2528372", "GSM2528373", "GSM2528374", "GSM2528375", "GSM2528376", "GSM2528377", "GSM2528378", "GSM2528379", "GSM2528380", "GSM2528381", "GSM2528382", "GSM2528383", "GSM2528384", "GSM2528385", "GSM2528386", "GSM2528387", "GSM2528388", "GSM2528389", "GSM2528390", "GSM2528391", "GSM2528392", "GSM2528393", "GSM2528394", "GSM2528395", "GSM2528396", "GSM2528397", "GSM2528398", "GSM2528399", "GSM2528400", "GSM2528401", "GSM2528402", "GSM2528403", "GSM2528404", "GSM2528405", "GSM2528406", "GSM2528407", "GSM2528408", "GSM2528409", "GSM2528410", "GSM2528411", "GSM2528412", "GSM2528413", "GSM2528414", "GSM2528415", "GSM2528416", "GSM2528417", "GSM2528418", "GSM2528419", "GSM2528420", "GSM2528421", "GSM2528422", "GSM2528423", "GSM2528424", "GSM2528425", "GSM2528426", "GSM2528427", "GSM2528428", "GSM2528429", "GSM2528430", "GSM2528431", "GSM2528432", "GSM2528433", "GSM2528434", "GSM2528435", "GSM2528436", "GSM2528437", "GSM2528438", "GSM2528439", "GSM2528440", "GSM2528441", "GSM2528442", "GSM2528443", "GSM2528444", "GSM2528445", "GSM2528446", "GSM2528447", "GSM2528448", "GSM2528449", "GSM2528450", "GSM2528451", "GSM2528452", "GSM2528453", "GSM2528454", "GSM2528455", "GSM2528456", "GSM2528457", "GSM2528458", "GSM2528459", "GSM2528460", "GSM2528461", "GSM2528462", "GSM2528463", "GSM2528464", "GSM2528465", "GSM2528466", "GSM2528467", "GSM2528468", "GSM2528469", "GSM2528470", "GSM2528471", "GSM2528472", "GSM2528473", "GSM2528474", "GSM2528475", "GSM2528476", "GSM2528477", "GSM2528478", "GSM2528479", "GSM2528480", "GSM2528481", "GSM2528482", "GSM2528483", "GSM2528484", "GSM2528485", "GSM2528486", "GSM2528487", "GSM2528488", "GSM2528489", "GSM2528490", "GSM2528491", "GSM2528492", "GSM2528493", "GSM2528494", "GSM2528495", "GSM2528496", "GSM2528497", "GSM2528498", "GSM2528499", "GSM2528500", "GSM2528501", "GSM2528502", "GSM2528503", "GSM2528504", "GSM2528505", "GSM2528506", "GSM2528507", "GSM2528508", "GSM2528509", "GSM2528510", "GSM2528511", "GSM2528512", "GSM2528513", "GSM2528514", "GSM2528515", "GSM2528516", "GSM2528517", "GSM2528518", "GSM2528519", "GSM2528520", "GSM2528521", "GSM2528522", "GSM2528523", "GSM2528524", "GSM2528525", "GSM2528526", "GSM2528527", "GSM2528528", "GSM2528529", "GSM2528530", "GSM2528531", "GSM2528532", "GSM2528533", "GSM2528534", "GSM2528535", "GSM2528536", "GSM2528537", "GSM2528538", "GSM2528539", "GSM2528540", "GSM2528541", "GSM2528542", "GSM2528543", "GSM2528544", "GSM2528545", "GSM2528546", "GSM2528547", "GSM2528548", "GSM2528549", "GSM2528550", "GSM2528551", "GSM2528552", "GSM2528553", "GSM2528554", "GSM2528555", "GSM2528556", "GSM2528557", "GSM2528558", "GSM2528559", "GSM2528560", "GSM2528561", "GSM2528562", "GSM2528563", "GSM2528564", "GSM2528565", "GSM2528566", "GSM2528567", "GSM2528568", "GSM2528569", "GSM2528570", "GSM2528571", "GSM2528572", "GSM2528573", "GSM2528574", "GSM2528575", "GSM2528576", "GSM2528577", "GSM2528578", "GSM2528579", "GSM2528580", "GSM2528581", "GSM2528582", "GSM2528583", "GSM2528584", "GSM2528585", "GSM2528586", "GSM2528587", "GSM2528588", "GSM2528589", "GSM2528590", "GSM2528591", "GSM2528592", "GSM2528593", "GSM2528594", "GSM2528595", "GSM2528596", "GSM2528597", "GSM2528598", "GSM2528599", "GSM2528600", "GSM2528601", "GSM2528602", "GSM2528603", "GSM2528604", "GSM2528605", "GSM2528606", "GSM2528607", "GSM2528608", "GSM2528609", "GSM2528610", "GSM2528611", "GSM2528612", "GSM2528613", "GSM2528614", "GSM2528615", "GSM2528616", "GSM2528617", "GSM2528618", "GSM2528619", "GSM2528620", "GSM2528621", "GSM2528622", "GSM2528623", "GSM2528624", "GSM2528625", "GSM2528626", "GSM2528627", "GSM2528628", "GSM2528629", "GSM2528630", "GSM2528631", "GSM2528632", "GSM2528633", "GSM2528634", "GSM2528635", "GSM2528636", "GSM2528637", "GSM2528638", "GSM2528639", "GSM2528640", "GSM2528641", "GSM2528642", "GSM2528643", "GSM2528644", "GSM2528645", "GSM2528646", "GSM2528647", "GSM2528648", "GSM2528649", "GSM2528650", "GSM2528651", "GSM2528652", "GSM2528653", "GSM2528654", "GSM2528655", "GSM2528656", "GSM2528657", "GSM2528658", "GSM2528659", "GSM2528660", "GSM2528661", "GSM2528662", "GSM2528663", "GSM2528664", "GSM2528665", "GSM2528666", "GSM2528667", "GSM2528668", "GSM2528669", "GSM2528670", "GSM2528671", "GSM2528672", "GSM2528673", "GSM2528674", "GSM2528675", "GSM2528676", "GSM2528677", "GSM2528678", "GSM2528679", "GSM2528680", "GSM2528681", "GSM2528682", "GSM2528683", "GSM2528684", "GSM2528685", "GSM2528686", "GSM2528687", "GSM2528688", "GSM2528689", "GSM2528690", "GSM2528691", "GSM2528692", "GSM2528693", "GSM2528694", "GSM2528695", "GSM2528696", "GSM2528697", "GSM2528698", "GSM2528699", "GSM2528700", "GSM2528701", "GSM2528702", "GSM2528703", "GSM2528704", "GSM2528705", "GSM2528706", "GSM2528707", "GSM2528708", "GSM2528709", "GSM2528710", "GSM2528711", "GSM2528712", "GSM2528713", "GSM2528714", "GSM2528715", "GSM2528716", "GSM2528717", "GSM2528718", "GSM2528719", "GSM2528720", "GSM2528721", "GSM2528722", "GSM2528723", "GSM2528724", "GSM2528725", "GSM2528726", "GSM2528727", "GSM2528728", "GSM2528729", "GSM2528730", "GSM2528731", "GSM2528732", "GSM2528733", "GSM2528734", "GSM2528735", "GSM2528736", "GSM2528737", "GSM2528738", "GSM2528739", "GSM2528740", "GSM2528741", "GSM2528742", "GSM2528743", "GSM2528744", "GSM2528745", "GSM2528746", "GSM2528747", "GSM2528748", "GSM2528749", "GSM2528750", "GSM2528751", "GSM2528752", "GSM2528753", "GSM2528754", "GSM2528755", "GSM2528756", "GSM2528757", "GSM2528758", "GSM2528759", "GSM2528760", "GSM2528761", "GSM2528762", "GSM2528763", "GSM2528764", "GSM2528765", "GSM2528766", "GSM2528767", "GSM2528768", "GSM2528769", "GSM2528770", "GSM2528771", "GSM2528772", "GSM2528773", "GSM2528774", "GSM2528775", "GSM2528776", "GSM2528777", "GSM2528778", "GSM2528779", "GSM2528780", "GSM2528781", "GSM2528782", "GSM2528783", "GSM2528784", "GSM2528785", "GSM2528786", "GSM2528787", "GSM2528788", "GSM2528789", "GSM2528790", "GSM2528791", "GSM2528792", "GSM2528793", "GSM2528794", "GSM2528795", "GSM2528796", "GSM2528797", "GSM2528798", "GSM2528799", "GSM2528800", "GSM2528801", "GSM2528802", "GSM2528803", "GSM2528804", "GSM2528805", "GSM2528806", "GSM2528807", "GSM2528808", "GSM2528809", "GSM2528810", "GSM2528811", "GSM2528812", "GSM2528813", "GSM2528814", "GSM2528815", "GSM2528816", "GSM2528817", "GSM2528818", "GSM2528819", "GSM2528820", "GSM2528821", "GSM2528822", "GSM2528823", "GSM2528824", "GSM2528825", "GSM2528826", "GSM2528827", "GSM2528828", "GSM2528829", "GSM2528830", "GSM2528831", "GSM2528832", "GSM2528833", "GSM2528834", "GSM2528835", "GSM2528836", "GSM2528837", "GSM2528838", "GSM2528839", "GSM2528840", "GSM2528841", "GSM2528842", "GSM2528843", "GSM2528844", "GSM2528845", "GSM2528846", "GSM2528847", "GSM2528848", "GSM2528849", "GSM2528850", "GSM2528851", "GSM2528852", "GSM2528853", "GSM2528854", "GSM2528855", "GSM2528856", "GSM2528857", "GSM2528858", "GSM2528859", "GSM2528860", "GSM2528861", "GSM2528862", "GSM2528863", "GSM2528864", "GSM2528865", "GSM2528866", "GSM2528867", "GSM2528868", "GSM2528869", "GSM2528870", "GSM2528871", "GSM2528872", "GSM2528873", "GSM2528874", "GSM2528875", "GSM2528876", "GSM2528877", "GSM2528878", "GSM2528879", "GSM2528880", "GSM2528881", "GSM2528882", "GSM2528883", "GSM2528884", "GSM2528885", "GSM2528886", "GSM2528887", "GSM2528888", "GSM2528889", "GSM2528890", "GSM2528891", "GSM2528892", "GSM2528893", "GSM2528894", "GSM2528895", "GSM2528896", "GSM2528897", "GSM2528898", "GSM2528899", "GSM2528900", "GSM2528901", "GSM2528902", "GSM2528903", "GSM2528904", "GSM2528905", "GSM2528906", "GSM2528907", "GSM2528908", "GSM2528909", "GSM2528910", "GSM2528911", "GSM2528912", "GSM2528913", "GSM2528914", "GSM2528915", "GSM2528916", "GSM2528917", "GSM2528918", "GSM2528919", "GSM2528920", "GSM2528921", "GSM2528922", "GSM2528923", "GSM2528924", "GSM2528925", "GSM2528926", "GSM2528927", "GSM2528928", "GSM2528929", "GSM2528930", "GSM2528931", "GSM2528932", "GSM2528933", "GSM2528934", "GSM2528935", "GSM2528936", "GSM2528937", "GSM2528938", "GSM2528939", "GSM2528940", "GSM2528941", "GSM2528942", "GSM2528943", "GSM2528944", "GSM2528945", "GSM2528946", "GSM2528947", "GSM2528948", "GSM2528949", "GSM2528950", "GSM2528951", "GSM2528952", "GSM2528953", "GSM2528954", "GSM2528955", "GSM2528956", "GSM2528957", "GSM2528958", "GSM2528959", "GSM2528960", "GSM2528961", "GSM2528962", "GSM2528963", "GSM2528964", "GSM2528965", "GSM2528966", "GSM2528967", "GSM2528968", "GSM2528969", "GSM2528970", "GSM2528971", "GSM2528972", "GSM2528973", "GSM2528974", "GSM2528975", "GSM2528976", "GSM2528977", "GSM2528978", "GSM2528979", "GSM2528980", "GSM2528981", "GSM2528982", "GSM2528983", "GSM2528984", "GSM2528985", "GSM2528986", "GSM2528987", "GSM2528988", "GSM2528989", "GSM2528990", "GSM2528991", "GSM2528992", "GSM2528993", "GSM2528994", "GSM2528995", "GSM2528996", "GSM2528997", "GSM2528998", "GSM2528999", "GSM2529000", "GSM2529001", "GSM2529002", "GSM2529003", "GSM2529004", "GSM2529005", "GSM2529006", "GSM2529007", "GSM2529008", "GSM2529009", "GSM2529010", "GSM2529011", "GSM2529012", "GSM2529013", "GSM2529014", "GSM2529015", "GSM2529016", "GSM2529017", "GSM2529018", "GSM2529019", "GSM2529020", "GSM2529021", "GSM2529022", "GSM2529023", "GSM2529024", "GSM2529025", "GSM2529026", "GSM2529027", "GSM2529028", "GSM2529029", "GSM2529030", "GSM2529031", "GSM2529032", "GSM2529033", "GSM2529034", "GSM2529035", "GSM2529036", "GSM2529037", "GSM2529038", "GSM2529039", "GSM2529040", "GSM2529041", "GSM2529042", "GSM2529043", "GSM2529044", "GSM2529045", "GSM2529046", "GSM2529047", "GSM2529048", "GSM2529049", "GSM2529050", "GSM2529051", "GSM2529052", "GSM2529053", "GSM2529054", "GSM2529055", "GSM2529056", "GSM2529057", "GSM2529058", "GSM2529059", "GSM2529060", "GSM2529061", "GSM2529062", "GSM2529063", "GSM2529064", "GSM2529065", "GSM2529066", "GSM2529067", "GSM2529068", "GSM2529069", "GSM2529070", "GSM2529071", "GSM2529072", "GSM2529073", "GSM2529074", "GSM2529075", "GSM2529076", "GSM2529077", "GSM2529078", "GSM2529079", "GSM2529080", "GSM2529081", "GSM2529082", "GSM2529083", "GSM2529084", "GSM2529085", "GSM2529086", "GSM2529087", "GSM2529088", "GSM2529089", "GSM2529090", "GSM2529091", "GSM2529092", "GSM2529093", "GSM2529094", "GSM2529095", "GSM2529096", "GSM2529097", "GSM2529098", "GSM2529099", "GSM2529100", "GSM2529101", "GSM2529102", "GSM2529103", "GSM2529104", "GSM2529105", "GSM2529106", "GSM2529107", "GSM2529108", "GSM2529109", "GSM2529110", "GSM2529111", "GSM2529112", "GSM2529113", "GSM2529114", "GSM2529115", "GSM2529116", "GSM2529117", "GSM2529118", "GSM2529119", "GSM2529120", "GSM2529121", "GSM2529122", "GSM2529123", "GSM2529124", "GSM2529125", "GSM2529126", "GSM2529127", "GSM2529128", "GSM2529129", "GSM2529130", "GSM2529131", "GSM2529132", "GSM2529133", "GSM2529134", "GSM2529135", "GSM2529136", "GSM2529137", "GSM2529138", "GSM2529139", "GSM2529140", "GSM2529141", "GSM2529142", "GSM2529143", "GSM2529144", "GSM2529145", "GSM2529146", "GSM2529147", "GSM2529148", "GSM2529149", "GSM2529150", "GSM2529151", "GSM2529152", "GSM2529153", "GSM2529154", "GSM2529155", "GSM2529156", "GSM2529157", "GSM2529158", "GSM2529159", "GSM2529160", "GSM2529161", "GSM2529162", "GSM2529163", "GSM2529164", "GSM2529165", "GSM2529166", "GSM2529167", "GSM2529168", "GSM2529169", "GSM2529170", "GSM2529171", "GSM2529172", "GSM2529173", "GSM2529174", "GSM2529175", "GSM2529176", "GSM2529177", "GSM2529178", "GSM2529179", "GSM2529180", "GSM2529181", "GSM2529182", "GSM2529183", "GSM2529184", "GSM2529185", "GSM2529186", "GSM2529187", "GSM2529188", "GSM2529189", "GSM2529190", "GSM2529191", "GSM2529192", "GSM2529193", "GSM2529194", "GSM2529195", "GSM2529196", "GSM2529197", "GSM2529198", "GSM2529199", "GSM2529200", "GSM2529201", "GSM2529202", "GSM2529203", "GSM2529204", "GSM2529205", "GSM2529206", "GSM2529207", "GSM2529208", "GSM2529209", "GSM2529210", "GSM2529211", "GSM2529212", "GSM2529213", "GSM2529214", "GSM2529215", "GSM2529216", "GSM2529217", "GSM2529218", "GSM2529219", "GSM2529220", "GSM2529221", "GSM2529222", "GSM2529223", "GSM2529224", "GSM2529225", "GSM2529226", "GSM2529227", "GSM2529228", "GSM2529229", "GSM2529230", "GSM2529231", "GSM2529232", "GSM2529233", "GSM2529234", "GSM2529235", "GSM2529236", "GSM2529237", "GSM2529238", "GSM2529239", "GSM2529240", "GSM2529241", "GSM2529242", "GSM2529243", "GSM2529244", "GSM2529245", "GSM2529246", "GSM2529247", "GSM2529248", "GSM2529249", "GSM2529250", "GSM2529251", "GSM2529252", "GSM2529253", "GSM2529254", "GSM2529255", "GSM2529256", "GSM2529257", "GSM2529258", "GSM2529259", "GSM2529260", "GSM2529261", "GSM2529262", "GSM2529263", "GSM2529264", "GSM2529265", "GSM2529266", "GSM2529267", "GSM2529268", "GSM2529269", "GSM2529270", "GSM2529271", "GSM2529272", "GSM2529273", "GSM2529274", "GSM2529275", "GSM2529276", "GSM2529277", "GSM2529278", "GSM2529279", "GSM2529280", "GSM2529281", "GSM2529282", "GSM2529283", "GSM2529284", "GSM2529285", "GSM2529286", "GSM2529287", "GSM2529288", "GSM2529289", "GSM2529290", "GSM2529291", "GSM2529292", "GSM2529293", "GSM2529294", "GSM2529295", "GSM2529296", "GSM2529297", "GSM2529298", "GSM2529299", "GSM2529300", "GSM2529301", "GSM2529302", "GSM2529303", "GSM2529304", "GSM2529305", "GSM2529306", "GSM2529307", "GSM2529308", "GSM2529309", "GSM2529310", "GSM2529311", "GSM2529312", "GSM2529313", "GSM2529314", "GSM2529315", "GSM2529316", "GSM2529317", "GSM2529318", "GSM2529319", "GSM2529320", "GSM2529321", "GSM2529322", "GSM2529323", "GSM2529324", "GSM2529325", "GSM2529326", "GSM2529327", "GSM2529328", "GSM2529329", "GSM2529330", "GSM2529331", "GSM2529332", "GSM2529333", "GSM2529334", "GSM2529335", "GSM2529336", "GSM2529337", "GSM2529338", "GSM2529339", "GSM2529340", "GSM2529341", "GSM2529342", "GSM2529343", "GSM2529344", "GSM2529345", "GSM2529346", "GSM2529347", "GSM2529348", "GSM2529349", "GSM2529350", "GSM2529351", "GSM2529352", "GSM2529353", "GSM2529354", "GSM2529355", "GSM2529356", "GSM2529357", "GSM2529358", "GSM2529359", "GSM2529360", "GSM2529361", "GSM2529362", "GSM2529363", "GSM2529364", "GSM2529365", "GSM2529366", "GSM2529367", "GSM2529368", "GSM2529369", "GSM2529370", "GSM2529371", "GSM2529372", "GSM2529373", "GSM2529374", "GSM2529375", "GSM2529376", "GSM2529377", "GSM2529378", "GSM2529379", "GSM2529380", "GSM2529381", "GSM2529382", "GSM2529383", "GSM2529384", "GSM2529385", "GSM2529386", "GSM2529387", "GSM2529388", "GSM2529389", "GSM2529390", "GSM2529391", "GSM2529392", "GSM2529393", "GSM2529394", "GSM2529395", "GSM2529396", "GSM2529397", "GSM2529398", "GSM2529399", "GSM2529400", "GSM2529401", "GSM2529402", "GSM2529403", "GSM2529404", "GSM2529405", "GSM2529406", "GSM2529407", "GSM2529408", "GSM2529409", "GSM2529410", "GSM2529411", "GSM2529412", "GSM2529413", "GSM2529414", "GSM2529415", "GSM2529416", "GSM2529417", "GSM2529418", "GSM2529419", "GSM2529420", "GSM2529421", "GSM2529422", "GSM2529423", "GSM2529424", "GSM2529425", "GSM2529426", "GSM2529427", "GSM2529428", "GSM2529429", "GSM2529430", "GSM2529431", "GSM2529432", "GSM2529433", "GSM2529434", "GSM2529435", "GSM2529436", "GSM2529437", "GSM2529438", "GSM2529439", "GSM2529440", "GSM2529441", "GSM2529442", "GSM2529443", "GSM2529444", "GSM2529445", "GSM2529446", "GSM2529447", "GSM2529448", "GSM2529449", "GSM2529450", "GSM2529451", "GSM2529452", "GSM2529453", "GSM2529454", "GSM2529455", "GSM2529456", "GSM2529457", "GSM2529458", "GSM2529459", "GSM2529460", "GSM2529461", "GSM2529462", "GSM2529463", "GSM2529464", "GSM2529465", "GSM2529466", "GSM2529467", "GSM2529468", "GSM2529469", "GSM2529470", "GSM2529471", "GSM2529472", "GSM2529473", "GSM2529474", "GSM2529475", "GSM2529476", "GSM2529477", "GSM2529478", "GSM2529479", "GSM2529480", "GSM2529481", "GSM2529482", "GSM2529483", "GSM2529484", "GSM2529485", "GSM2529486", "GSM2529487", "GSM2529488", "GSM2529489", "GSM2529490", "GSM2529491", "GSM2529492", "GSM2529493", "GSM2529494", "GSM2529495", "GSM2529496", "GSM2529497", "GSM2529498", "GSM2529499", "GSM2529500", "GSM2529501", "GSM2529502", "GSM2529503", "GSM2529504", "GSM2529505", "GSM2529506", "GSM2529507", "GSM2529508", "GSM2529509", "GSM2529510", "GSM2529511", "GSM2529512", "GSM2529513", "GSM2529514", "GSM2529515", "GSM2529516", "GSM2529517", "GSM2529518", "GSM2529519", "GSM2529520", "GSM2529521", "GSM2529522", "GSM2529523", "GSM2529524", "GSM2529525", "GSM2529526", "GSM2529527", "GSM2529528", "GSM2529529", "GSM2529530", "GSM2529531", "GSM2529532", "GSM2529533", "GSM2529534", "GSM2529535", "GSM2529536", "GSM2529537", "GSM2529538", "GSM2529539", "GSM2529540", "GSM2529541", "GSM2529542", "GSM2529543", "GSM2529544", "GSM2529545", "GSM2529546", "GSM2529547", "GSM2529548", "GSM2529549", "GSM2529550", "GSM2529551", "GSM2529552", "GSM2529553", "GSM2529554", "GSM2529555", "GSM2529556", "GSM2529557", "GSM2529558", "GSM2529559", "GSM2529560", "GSM2529561", "GSM2529562", "GSM2529563", "GSM2529564", "GSM2529565", "GSM2529566", "GSM2529567", "GSM2529568", "GSM2529569", "GSM2529570", "GSM2529571", "GSM2529572", "GSM2529573", "GSM2529574", "GSM2529575", "GSM2529576", "GSM2529577", "GSM2529578", "GSM2529579", "GSM2529580", "GSM2529581", "GSM2529582", "GSM2529583", "GSM2529584", "GSM2529585", "GSM2529586", "GSM2529587", "GSM2529588", "GSM2529589", "GSM2529590", "GSM2529591", "GSM2529592", "GSM2529593", "GSM2529594", "GSM2529595", "GSM2529596", "GSM2529597", "GSM2529598", "GSM2529599", "GSM2529600", "GSM2529601", "GSM2529602", "GSM2529603", "GSM2529604", "GSM2529605", "GSM2529606", "GSM2529607", "GSM2529608", "GSM2529609", "GSM2529610", "GSM2529611", "GSM2529612", "GSM2529613", "GSM2529614", "GSM2529615", "GSM2529616", "GSM2529617", "GSM2529618", "GSM2529619", "GSM2529620", "GSM2529621", "GSM2529622", "GSM2529623", "GSM2529624", "GSM2529625", "GSM2529626", "GSM2529627", "GSM2529628", "GSM2529629", "GSM2529630", "GSM2529631", "GSM2529632", "GSM2529633", "GSM2529634", "GSM2529635", "GSM2529636", "GSM2529637", "GSM2529638", "GSM2529639", "GSM2529640", "GSM2529641", "GSM2529642", "GSM2529643", "GSM2529644", "GSM2529645", "GSM2529646", "GSM2529647", "GSM2529648", "GSM2529649", "GSM2529650", "GSM2529651", "GSM2529652", "GSM2529653", "GSM2529654", "GSM2529655", "GSM2529656", "GSM2529657", "GSM2529658", "GSM2529659", "GSM2529660", "GSM2529661", "GSM2529662", "GSM2529663", "GSM2529664", "GSM2529665", "GSM2529666", "GSM2529667", "GSM2529668", "GSM2529669", "GSM2529670", "GSM2529671", "GSM2529672", "GSM2529673", "GSM2529674", "GSM2529675", "GSM2529676", "GSM2529677", "GSM2529678", "GSM2529679", "GSM2529680", "GSM2529681", "GSM2529682", "GSM2529683", "GSM2529684", "GSM2529685", "GSM2529686", "GSM2529687", "GSM2529688", "GSM2529689", "GSM2529690", "GSM2529691", "GSM2529692", "GSM2529693", "GSM2529694", "GSM2529695", "GSM2529696", "GSM2529697", "GSM2529698", "GSM2529699", "GSM2529700", "GSM2529701", "GSM2529702", "GSM2529703", "GSM2529704", "GSM2529705", "GSM2529706", "GSM2529707", "GSM2529708", "GSM2529709", "GSM2529710", "GSM2529711", "GSM2529712", "GSM2529713", "GSM2529714", "GSM2529715", "GSM2529716", "GSM2529717", "GSM2529718", "GSM2529719", "GSM2529720", "GSM2529721", "GSM2529722", "GSM2529723", "GSM2529724", "GSM2529725", "GSM2529726", "GSM2529727", "GSM2529728", "GSM2529729", "GSM2529730", "GSM2529731", "GSM2529732", "GSM2529733", "GSM2529734", "GSM2529735", "GSM2529736", "GSM2529737", "GSM2529738", "GSM2529739", "GSM2529740", "GSM2529741", "GSM2529742", "GSM2529743", "GSM2529744", "GSM2529745", "GSM2529746", "GSM2529747", "GSM2529748", "GSM2529749", "GSM2529750", "GSM2529751", "GSM2529752", "GSM2529753", "GSM2529754", "GSM2529755", "GSM2529756", "GSM2529757", "GSM2529758", "GSM2529759", "GSM2529760", "GSM2529761", "GSM2529762", "GSM2529763", "GSM2529764", "GSM2529765", "GSM2529766", "GSM2529767", "GSM2529768", "GSM2529769", "GSM2529770", "GSM2529771", "GSM2529772", "GSM2529773", "GSM2529774", "GSM2529775", "GSM2529776", "GSM2529777", "GSM2529778", "GSM2529779", "GSM2529780", "GSM2529781", "GSM2529782", "GSM2529783", "GSM2529784", "GSM2529785", "GSM2529786", "GSM2529787", "GSM2529788", "GSM2529789", "GSM2529790", "GSM2529791", "GSM2529792", "GSM2529793", "GSM2529794", "GSM2529795", "GSM2529796", "GSM2529797", "GSM2529798", "GSM2529799", "GSM2529800", "GSM2529801", "GSM2529802", "GSM2529803", "GSM2529804", "GSM2529805", "GSM2529806", "GSM2529807", "GSM2529808", "GSM2529809", "GSM2529810", "GSM2529811", "GSM2529812", "GSM2529813", "GSM2529814", "GSM2529815", "GSM2529816", "GSM2529817", "GSM2529818", "GSM2529819", "GSM2529820", "GSM2529821", "GSM2529822", "GSM2529823", "GSM2529824", "GSM2529825", "GSM2529826", "GSM2529827", "GSM2529828", "GSM2529829", "GSM2529830", "GSM2529831", "GSM2529832", "GSM2529833", "GSM2529834", "GSM2529835", "GSM2529836", "GSM2529837", "GSM2529838", "GSM2529839", "GSM2529840", "GSM2529841", "GSM2529842", "GSM2529843", "GSM2529844", "GSM2529845", "GSM2529846", "GSM2529847", "GSM2529848", "GSM2529849", "GSM2529850", "GSM2529851", "GSM2529852", "GSM2529853", "GSM2529854", "GSM2529855", "GSM2529856", "GSM2529857", "GSM2529858", "GSM2529859", "GSM2529860", "GSM2529861", "GSM2529862", "GSM2529863", "GSM2529864", "GSM2529865", "GSM2529866", "GSM2529867", "GSM2529868", "GSM2529869", "GSM2529870", "GSM2529871", "GSM2529872", "GSM2529873", "GSM2529874", "GSM2529875", "GSM2529876", "GSM2529877", "GSM2529878", "GSM2529879", "GSM2529880", "GSM2529881", "GSM2529882", "GSM2529883", "GSM2529884", "GSM2529885", "GSM2529886", "GSM2529887", "GSM2529888", "GSM2529889", "GSM2529890", "GSM2529891", "GSM2529892", "GSM2529893", "GSM2529894", "GSM2529895", "GSM2529896", "GSM2529897", "GSM2529898", "GSM2529899", "GSM2529900", "GSM2529901", "GSM2529902", "GSM2529903", "GSM2529904", "GSM2529905", "GSM2529906", "GSM2529907", "GSM2529908", "GSM2529909", "GSM2529910", "GSM2529911", "GSM2529912", "GSM2529913", "GSM2529914", "GSM2529915", "GSM2529916", "GSM2529917", "GSM2529918", "GSM2529919", "GSM2529920", "GSM2529921", "GSM2529922", "GSM2529923", "GSM2529924", "GSM2529925", "GSM2529926", "GSM2529927", "GSM2529928", "GSM2529929", "GSM2529930", "GSM2529931", "GSM2529932", "GSM2529933", "GSM2529934", "GSM2529935", "GSM2529936", "GSM2529937", "GSM2529938", "GSM2529939", "GSM2529940", "GSM2529941", "GSM2529942", "GSM2529943", "GSM2529944", "GSM2529945", "GSM2529946", "GSM2529947", "GSM2529948", "GSM2529949", "GSM2529950", "GSM2529951", "GSM2529952", "GSM2529953", "GSM2529954", "GSM2529955", "GSM2529956", "GSM2529957", "GSM2529958", "GSM2529959", "GSM2529960", "GSM2529961", "GSM2529962", "GSM2529963", "GSM2529964", "GSM2529965", "GSM2529966", "GSM2529967", "GSM2529968", "GSM2529969", "GSM2529970", "GSM2529971", "GSM2529972", "GSM2529973", "GSM2529974", "GSM2529975", "GSM2529976", "GSM2529977", "GSM2529978", "GSM2529979", "GSM2529980", "GSM2529981", "GSM2529982", "GSM2529983", "GSM2529984", "GSM2529985", "GSM2529986", "GSM2529987", "GSM2529988", "GSM2529989", "GSM2529990", "GSM2529991", "GSM2529992", "GSM2529993", "GSM2529994", "GSM2529995", "GSM2529996", "GSM2529997", "GSM2529998", "GSM2529999", "GSM2530000", "GSM2530001", "GSM2530002", "GSM2530003", "GSM2530004", "GSM2530005", "GSM2530006", "GSM2530007", "GSM2530008", "GSM2530009", "GSM2530010", "GSM2530011", "GSM2530012", "GSM2530013", "GSM2530014", "GSM2530015", "GSM2530016", "GSM2530017", "GSM2530018", "GSM2530019", "GSM2530020", "GSM2530021", "GSM2530022", "GSM2530023", "GSM2530024", "GSM2530025", "GSM2530026", "GSM2530027", "GSM2530028", "GSM2530029", "GSM2530030", "GSM2530031", "GSM2530032", "GSM2530033", "GSM2530034", "GSM2530035", "GSM2530036", "GSM2530037", "GSM2530038", "GSM2530039", "GSM2530040", "GSM2530041", "GSM2530042", "GSM2530043", "GSM2530044", "GSM2530045", "GSM2530046", "GSM2530047", "GSM2530048", "GSM2530049", "GSM2530050", "GSM2530051", "GSM2530052", "GSM2530053", "GSM2530054", "GSM2530055", "GSM2530056", "GSM2530057", "GSM2530058", "GSM2530059", "GSM2530060", "GSM2530061", "GSM2530062", "GSM2530063", "GSM2530064", "GSM2530065", "GSM2530066", "GSM2530067", "GSM2530068", "GSM2530069", "GSM2530070", "GSM2530071", "GSM2530072", "GSM2530073", "GSM2530074", "GSM2530075", "GSM2530076", "GSM2530077", "GSM2530078", "GSM2530079", "GSM2530080", "GSM2530081", "GSM2530082", "GSM2530083", "GSM2530084", "GSM2530085", "GSM2530086", "GSM2530087", "GSM2530088", "GSM2530089", "GSM2530090", "GSM2530091", "GSM2530092", "GSM2530093", "GSM2530094", "GSM2530095", "GSM2530096", "GSM2530097", "GSM2530098", "GSM2530099", "GSM2530100", "GSM2530101", "GSM2530102", "GSM2530103", "GSM2530104", "GSM2530105", "GSM2530106", "GSM2530107", "GSM2530108", "GSM2530109", "GSM2530110", "GSM2530111", "GSM2530112", "GSM2530113", "GSM2530114", "GSM2530115", "GSM2530116", "GSM2530117", "GSM2530118", "GSM2530119", "GSM2530120", "GSM2530121", "GSM2530122", "GSM2530123", "GSM2530124", "GSM2530125", "GSM2530126", "GSM2530127", "GSM2530128", "GSM2530129", "GSM2530130", "GSM2530131", "GSM2530132", "GSM2530133", "GSM2530134", "GSM2530135", "GSM2530136", "GSM2530137", "GSM2530138", "GSM2530139", "GSM2530140", "GSM2530141", "GSM2530142", "GSM2530143", "GSM2530144", "GSM2530145", "GSM2530146", "GSM2530147", "GSM2530148", "GSM2530149", "GSM2530150", "GSM2530151", "GSM2530152", "GSM2530153", "GSM2530154", "GSM2530155", "GSM2530156", "GSM2530157", "GSM2530158", "GSM2530159", "GSM2530160", "GSM2530161", "GSM2530162", "GSM2530163", "GSM2530164", "GSM2530165", "GSM2530166", "GSM2530167", "GSM2530168", "GSM2530169", "GSM2530170", "GSM2530171", "GSM2530172", "GSM2530173", "GSM2530174", "GSM2530175", "GSM2530176", "GSM2530177", "GSM2530178", "GSM2530179", "GSM2530180", "GSM2530181", "GSM2530182", "GSM2530183", "GSM2530184", "GSM2530185", "GSM2530186", "GSM2530187", "GSM2530188", "GSM2530189", "GSM2530190", "GSM2530191", "GSM2530192", "GSM2530193", "GSM2530194", "GSM2530195", "GSM2530196", "GSM2530197", "GSM2530198", "GSM2530199", "GSM2530200", "GSM2530201", "GSM2530202", "GSM2530203", "GSM2530204", "GSM2530205", "GSM2530206", "GSM2530207", "GSM2530208", "GSM2530209", "GSM2530210", "GSM2530211", "GSM2530212", "GSM2530213", "GSM2530214", "GSM2530215", "GSM2530216", "GSM2530217", "GSM2530218", "GSM2530219", "GSM2530220", "GSM2530221", "GSM2530222", "GSM2530223", "GSM2530224", "GSM2530225", "GSM2530226", "GSM2530227", "GSM2530228", "GSM2530229", "GSM2530230", "GSM2530231", "GSM2530232", "GSM2530233", "GSM2530234", "GSM2530235", "GSM2530236", "GSM2530237", "GSM2530238", "GSM2530239", "GSM2530240", "GSM2530241", "GSM2530242", "GSM2530243", "GSM2530244", "GSM2530245", "GSM2530246", "GSM2530247", "GSM2530248", "GSM2530249", "GSM2530250", "GSM2530251", "GSM2530252", "GSM2530253", "GSM2530254", "GSM2530255", "GSM2530256", "GSM2530257", "GSM2530258", "GSM2530259", "GSM2530260", "GSM2530261", "GSM2530262", "GSM2530263", "GSM2530264", "GSM2530265", "GSM2530266", "GSM2530267", "GSM2530268", "GSM2530269", "GSM2530270", "GSM2530271", "GSM2530272", "GSM2530273", "GSM2530274", "GSM2530275", "GSM2530276", "GSM2530277", "GSM2530278", "GSM2530279", "GSM2530280", "GSM2530281", "GSM2530282", "GSM2530283", "GSM2530284", "GSM2530285", "GSM2530286", "GSM2530287", "GSM2530288", "GSM2530289", "GSM2530290", "GSM2530291", "GSM2530292", "GSM2530293", "GSM2530294", "GSM2530295", "GSM2530296", "GSM2530297", "GSM2530298", "GSM2530299", "GSM2530300", "GSM2530301", "GSM2530302", "GSM2530303", "GSM2530304", "GSM2530305", "GSM2530306", "GSM2530307", "GSM2530308", "GSM2530309", "GSM2530310", "GSM2530311", "GSM2530312", "GSM2530313", "GSM2530314", "GSM2530315", "GSM2530316", "GSM2530317", "GSM2530318", "GSM2530319", "GSM2530320", "GSM2530321", "GSM2530322", "GSM2530323", "GSM2530324", "GSM2530325", "GSM2530326", "GSM2530327", "GSM2530328", "GSM2530329", "GSM2530330", "GSM2530331", "GSM2530332", "GSM2530333", "GSM2530334", "GSM2530335", "GSM2530336", "GSM2530337", "GSM2530338", "GSM2530339", "GSM2530340", "GSM2530341", "GSM2530342", "GSM2530343", "GSM2530344", "GSM2530345", "GSM2530346", "GSM2530347", "GSM2530348", "GSM2530349", "GSM2530350", "GSM2530351", "GSM2530352", "GSM2530353", "GSM2530354", "GSM2530355", "GSM2530356", "GSM2530357", "GSM2530358", "GSM2530359", "GSM2530360", "GSM2530361", "GSM2530362", "GSM2530363", "GSM2530364", "GSM2530365", "GSM2530366", "GSM2530367", "GSM2530368", "GSM2530369", "GSM2530370", "GSM2530371", "GSM2530372", "GSM2530373", "GSM2530374", "GSM2530375", "GSM2530376", "GSM2530377", "GSM2530378", "GSM2530379", "GSM2530380", "GSM2530381", "GSM2530382", "GSM2530383", "GSM2530384", "GSM2530385", "GSM2530386", "GSM2530387", "GSM2530388", "GSM2530389", "GSM2530390", "GSM2530391", "GSM2530392", "GSM2530393", "GSM2530394", "GSM2530395", "GSM2530396", "GSM2530397", "GSM2530398", "GSM2530399", "GSM2530400", "GSM2530401", "GSM2530402", "GSM2530403", "GSM2530404", "GSM2530405", "GSM2530406", "GSM2530407", "GSM2530408", "GSM2530409", "GSM2530410", "GSM2530411", "GSM2530412", "GSM2530413", "GSM2530414", "GSM2530415", "GSM2530416", "GSM2530417", "GSM2530418", "GSM2530419", "GSM2530420", "GSM2530421", "GSM2530422", "GSM2530423", "GSM2530424", "GSM2530425", "GSM2530426", "GSM2530427", "GSM2530428", "GSM2530429", "GSM2530430", "GSM2530431", "GSM2530432", "GSM2530433", "GSM2530434", "GSM2530435", "GSM2530436", "GSM2530437", "GSM2530438", "GSM2530439", "GSM2530440", "GSM2530441", "GSM2530442", "GSM2530443", "GSM2530444", "GSM2530445", "GSM2530446", "GSM2530447", "GSM2530448", "GSM2530449", "GSM2530450", "GSM2530451", "GSM2530452", "GSM2530453", "GSM2530454", "GSM2530455", "GSM2530456", "GSM2530457", "GSM2530458", "GSM2530459", "GSM2530460", "GSM2530461", "GSM2530462", "GSM2530463", "GSM2530464", "GSM2530465", "GSM2530466", "GSM2530467", "GSM2530468", "GSM2530469", "GSM2530470", "GSM2530471", "GSM2530472", "GSM2530473", "GSM2530474", "GSM2530475", "GSM2530476", "GSM2530477", "GSM2530478", "GSM2530479", "GSM2530480", "GSM2530481", "GSM2530482", "GSM2530483", "GSM2530484", "GSM2530485", "GSM2530486", "GSM2530487", "GSM2530488", "GSM2530489", "GSM2530490", "GSM2530491", "GSM2530492", "GSM2530493", "GSM2530494", "GSM2530495", "GSM2530496", "GSM2530497", "GSM2530498", "GSM2530499", "GSM2530500", "GSM2530501", "GSM2530502", "GSM2530503", "GSM2530504", "GSM2530505", "GSM2530506", "GSM2530507", "GSM2530508", "GSM2530509", "GSM2530510", "GSM2530511", "GSM2530512", "GSM2530513", "GSM2530514", "GSM2530515", "GSM2530516", "GSM2530517", "GSM2530518", "GSM2530519", "GSM2530520", "GSM2530521", "GSM2530522", "GSM2530523", "GSM2530524", "GSM2530525", "GSM2530526", "GSM2530527", "GSM2530528", "GSM2530529", "GSM2530530", "GSM2530531", "GSM2530532", "GSM2530533", "GSM2530534", "GSM2530535", "GSM2530536", "GSM2530537", "GSM2530538", "GSM2530539", "GSM2530540", "GSM2530541", "GSM2530542", "GSM2530543", "GSM2530544", "GSM2530545", "GSM2530546", "GSM2530547", "GSM2530548", "GSM2530549", "GSM2530550", "GSM2530551", "GSM2530552", "GSM2530553", "GSM2530554", "GSM2530555", "GSM2530556", "GSM2530557", "GSM2530558", "GSM2530559", "GSM2530560", "GSM2530561", "GSM2530562", "GSM2530563", "GSM2530564", "GSM2530565", "GSM2530566", "GSM2530567", "GSM2530568", "GSM2530569", "GSM2530570", "GSM2530571", "GSM2530572", "GSM2530573", "GSM2530574", "GSM2530575", "GSM2530576", "GSM2530577", "GSM2530578", "GSM2530579", "GSM2530580", "GSM2530581", "GSM2530582", "GSM2530583", "GSM2530584", "GSM2530585", "GSM2530586", "GSM2530587", "GSM2530588", "GSM2530589", "GSM2530590", "GSM2530591", "GSM2530592", "GSM2530593", "GSM2530594", "GSM2530595", "GSM2530596", "GSM2530597", "GSM2530598", "GSM2530599", "GSM2530600", "GSM2530601", "GSM2530602", "GSM2530603", "GSM2530604", "GSM2530605", "GSM2530606", "GSM2530607", "GSM2530608", "GSM2530609", "GSM2530610", "GSM2530611", "GSM2530612", "GSM2530613", "GSM2530614", "GSM2530615", "GSM2530616", "GSM2530617", "GSM2530618", "GSM2530619", "GSM2530620", "GSM2530621", "GSM2530622", "GSM2530623", "GSM2530624", "GSM2530625", "GSM2530626", "GSM2530627", "GSM2530628", "GSM2530629", "GSM2530630", "GSM2530631", "GSM2530632", "GSM2530633", "GSM2530634", "GSM2530635", "GSM2530636", "GSM2530637", "GSM2530638", "GSM2530639", "GSM2530640", "GSM2530641", "GSM2530642", "GSM2530643", "GSM2530644", "GSM2530645", "GSM2530646", "GSM2530647", "GSM2530648", "GSM2530649", "GSM2530650", "GSM2530651", "GSM2530652", "GSM2530653", "GSM2530654", "GSM2530655", "GSM2530656", "GSM2530657", "GSM2530658", "GSM2530659", "GSM2530660", "GSM2530661", "GSM2530662", "GSM2530663", "GSM2530664", "GSM2530665", "GSM2530666", "GSM2530667", "GSM2530668", "GSM2530669", "GSM2530670", "GSM2530671", "GSM2530672", "GSM2530673", "GSM2530674", "GSM2530675", "GSM2530676", "GSM2530677", "GSM2530678", "GSM2530679", "GSM2530680", "GSM2530681", "GSM2530682", "GSM2530683", "GSM2530684", "GSM2530685", "GSM2530686", "GSM2530687", "GSM2530688", "GSM2530689", "GSM2530690", "GSM2530691", "GSM2530692", "GSM2530693", "GSM2530694", "GSM2530695", "GSM2530696", "GSM2530697", "GSM2530698", "GSM2530699", "GSM2530700", "GSM2530701", "GSM2530702", "GSM2530703", "GSM2530704", "GSM2530705", "GSM2530706", "GSM2530707", "GSM2530708", "GSM2530709", "GSM2530710", "GSM2530711", "GSM2530712", "GSM2530713", "GSM2530714", "GSM2530715", "GSM2530716", "GSM2530717", "GSM2530718", "GSM2530719", "GSM2530720", "GSM2530721", "GSM2530722", "GSM2530723", "GSM2530724", "GSM2530725", "GSM2530726", "GSM2530727", "GSM2530728", "GSM2530729", "GSM2530730", "GSM2530731", "GSM2530732", "GSM2530733", "GSM2530734", "GSM2530735", "GSM2530736", "GSM2530737", "GSM2530738", "GSM2530739", "GSM2530740", "GSM2530741", "GSM2530742", "GSM2530743", "GSM2530744", "GSM2530745", "GSM2530746", "GSM2530747", "GSM2530748", "GSM2530749", "GSM2530750", "GSM2530751", "GSM2530752", "GSM2530753", "GSM2530754", "GSM2530755", "GSM2530756", "GSM2530757", "GSM2530758", "GSM2530759", "GSM2530760", "GSM2530761", "GSM2530762", "GSM2530763", "GSM2530764", "GSM2530765", "GSM2530766", "GSM2530767", "GSM2530768", "GSM2530769", "GSM2530770", "GSM2530771", "GSM2530772", "GSM2530773", "GSM2530774", "GSM2530775", "GSM2530776", "GSM2530777", "GSM2530778", "GSM2530779", "GSM2530780", "GSM2530781", "GSM2530782", "GSM2530783", "GSM2530784", "GSM2530785", "GSM2530786", "GSM2530787", "GSM2530788", "GSM2530789", "GSM2530790", "GSM2530791", "GSM2530792", "GSM2530793", "GSM2530794", "GSM2530795", "GSM2530796", "GSM2530797", "GSM2530798", "GSM2530799", "GSM2530800", "GSM2530801", "GSM2530802", "GSM2530803", "GSM2530804", "GSM2530805", "GSM2530806", "GSM2530807", "GSM2530808", "GSM2530809", "GSM2530810", "GSM2530811", "GSM2530812", "GSM2530813", "GSM2530814", "GSM2530815", "GSM2530816", "GSM2530817", "GSM2530818", "GSM2530819", "GSM2530820", "GSM2530821", "GSM2530822", "GSM2530823", "GSM2530824", "GSM2530825", "GSM2530826", "GSM2530827", "GSM2530828", "GSM2530829", "GSM2530830", "GSM2530831", "GSM2530832", "GSM2530833", "GSM2530834", "GSM2530835", "GSM2530836", "GSM2530837", "GSM2530838", "GSM2530839", "GSM2530840", "GSM2530841", "GSM2530842", "GSM2530843", "GSM2530844", "GSM2530845", "GSM2530846", "GSM2530847", "GSM2530848", "GSM2530849", "GSM2530850", "GSM2530851", "GSM2530852", "GSM2530853", "GSM2530854", "GSM2530855", "GSM2530856", "GSM2530857", "GSM2530858", "GSM2530859", "GSM2530860", "GSM2530861", "GSM2530862", "GSM2530863", "GSM2530864", "GSM2530865", "GSM2530866", "GSM2530867", "GSM2530868", "GSM2530869", "GSM2530870", "GSM2530871", "GSM2530872", "GSM2530873", "GSM2530874", "GSM2530875", "GSM2530876", "GSM2530877", "GSM2530878", "GSM2530879", "GSM2530880", "GSM2530881", "GSM2530882", "GSM2530883", "GSM2530884", "GSM2530885", "GSM2530886", "GSM2530887", "GSM2530888", "GSM2530889", "GSM2530890", "GSM2530891", "GSM2530892", "GSM2530893", "GSM2530894", "GSM2530895", "GSM2530896", "GSM2530897", "GSM2530898", "GSM2530899", "GSM2530900", "GSM2530901", "GSM2530902", "GSM2530903", "GSM2530904", "GSM2530905", "GSM2530906", "GSM2530907", "GSM2530908", "GSM2530909", "GSM2530910", "GSM2530911", "GSM2530912", "GSM2530913", "GSM2530914", "GSM2530915", "GSM2530916", "GSM2530917", "GSM2530918", "GSM2530919", "GSM2530920", "GSM2530921", "GSM2530922", "GSM2530923", "GSM2530924", "GSM2530925", "GSM2530926", "GSM2530927", "GSM2530928", "GSM2530929", "GSM2530930", "GSM2530931", "GSM2530932", "GSM2530933", "GSM2530934", "GSM2530935", "GSM2530936", "GSM2530937", "GSM2530938", "GSM2530939", "GSM2530940", "GSM2530941", "GSM2530942", "GSM2530943", "GSM2530944", "GSM2530945", "GSM2530946", "GSM2530947", "GSM2530948", "GSM2530949", "GSM2530950", "GSM2530951", "GSM2530952", "GSM2530953", "GSM2530954", "GSM2530955", "GSM2530956", "GSM2530957", "GSM2530958", "GSM2530959", "GSM2530960", "GSM2530961", "GSM2530962", "GSM2530963", "GSM2530964", "GSM2530965", "GSM2530966", "GSM2530967", "GSM2530968", "GSM2530969", "GSM2530970", "GSM2530971", "GSM2530972", "GSM2530973", "GSM2530974", "GSM2530975", "GSM2530976", "GSM2530977", "GSM2530978", "GSM2530979", "GSM2530980", "GSM2530981", "GSM2530982", "GSM2530983", "GSM2530984", "GSM2530985", "GSM2530986", "GSM2530987", "GSM2530988", "GSM2530989", "GSM2530990", "GSM2530991", "GSM2530992", "GSM2530993", "GSM2530994", "GSM2530995", "GSM2530996", "GSM2530997", "GSM2530998", "GSM2530999", "GSM2531000", "GSM2531001", "GSM2531002", "GSM2531003", "GSM2531004", "GSM2531005", "GSM2531006", "GSM2531007", "GSM2531008", "GSM2531009", "GSM2531010", "GSM2531011", "GSM2531012", "GSM2531013", "GSM2531014", "GSM2531015", "GSM2531016", "GSM2531017", "GSM2531018", "GSM2531019", "GSM2531020", "GSM2531021", "GSM2531022", "GSM2531023", "GSM2531024", "GSM2531025", "GSM2531026", "GSM2531027", "GSM2531028", "GSM2531029", "GSM2531030", "GSM2531031", "GSM2531032", "GSM2531033", "GSM2531034", "GSM2531035", "GSM2531036", "GSM2531037", "GSM2531038", "GSM2531039", "GSM2531040", "GSM2531041", "GSM2531042", "GSM2531043", "GSM2531044", "GSM2531045", "GSM2531046", "GSM2531047", "GSM2531048", "GSM2531049", "GSM2531050", "GSM2531051", "GSM2531052", "GSM2531053", "GSM2531054", "GSM2531055", "GSM2531056", "GSM2531057", "GSM2531058", "GSM2531059", "GSM2531060", "GSM2531061", "GSM2531062", "GSM2531063", "GSM2531064", "GSM2531065", "GSM2531066", "GSM2531067", "GSM2531068", "GSM2531069", "GSM2531070", "GSM2531071", "GSM2531072", "GSM2531073", "GSM2531074", "GSM2531075", "GSM2531076", "GSM2531077", "GSM2531078", "GSM2531079", "GSM2531080", "GSM2531081", "GSM2531082", "GSM2531083", "GSM2531084", "GSM2531085", "GSM2531086", "GSM2531087", "GSM2531088", "GSM2531089", "GSM2531090", "GSM2531091", "GSM2531092", "GSM2531093", "GSM2531094", "GSM2531095", "GSM2531096", "GSM2531097", "GSM2531098", "GSM2531099", "GSM2531100", "GSM2531101", "GSM2531102", "GSM2531103", "GSM2531104", "GSM2531105", "GSM2531106", "GSM2531107", "GSM2531108", "GSM2531109", "GSM2531110", "GSM2531111", "GSM2531112", "GSM2531113", "GSM2531114", "GSM2531115", "GSM2531116", "GSM2531117", "GSM2531118", "GSM2531119", "GSM2531120", "GSM2531121", "GSM2531122", "GSM2531123", "GSM2531124", "GSM2531125", "GSM2531126", "GSM2531127", "GSM2531128", "GSM2531129", "GSM2531130", "GSM2531131", "GSM2531132", "GSM2531133", "GSM2531134", "GSM2531135", "GSM2531136", "GSM2531137", "GSM2531138", "GSM2531139", "GSM2531140", "GSM2531141", "GSM2531142", "GSM2531143", "GSM2531144", "GSM2531145", "GSM2531146", "GSM2531147", "GSM2531148", "GSM2531149", "GSM2531150", "GSM2531151", "GSM2531152", "GSM2531153", "GSM2531154", "GSM2531155", "GSM2531156", "GSM2531157", "GSM2531158", "GSM2531159", "GSM2531160", "GSM2531161", "GSM2531162", "GSM2531163", "GSM2531164", "GSM2531165", "GSM2531166", "GSM2531167", "GSM2531168", "GSM2531169", "GSM2531170", "GSM2531171", "GSM2531172", "GSM2531173", "GSM2531174", "GSM2531175", "GSM2531176", "GSM2531177", "GSM2531178", "GSM2531179", "GSM2531180", "GSM2531181", "GSM2531182", "GSM2531183", "GSM2531184", "GSM2531185", "GSM2531186", "GSM2531187", "GSM2531188", "GSM2531189", "GSM2531190", "GSM2531191", "GSM2531192", "GSM2531193", "GSM2531194", "GSM2531195", "GSM2531196", "GSM2531197", "GSM2531198", "GSM2531199", "GSM2531200", "GSM2531201", "GSM2531202", "GSM2531203", "GSM2531204", "GSM2531205", "GSM2531206", "GSM2531207", "GSM2531208", "GSM2531209", "GSM2531210", "GSM2531211", "GSM2531212", "GSM2531213", "GSM2531214", "GSM2531215", "GSM2531216", "GSM2531217", "GSM2531218", "GSM2531219", "GSM2531220", "GSM2531221", "GSM2531222", "GSM2531223", "GSM2531224", "GSM2531225", "GSM2531226", "GSM2531227", "GSM2531228", "GSM2531229", "GSM2531230", "GSM2531231", "GSM2531232", "GSM2531233", "GSM2531234", "GSM2531235", "GSM2531236", "GSM2531237", "GSM2531238", "GSM2531239", "GSM2531240", "GSM2531241", "GSM2531242", "GSM2531243", "GSM2531244", "GSM2531245", "GSM2531246", "GSM2531247", "GSM2531248", "GSM2531249", "GSM2531250", "GSM2531251", "GSM2531252", "GSM2531253", "GSM2531254", "GSM2531255", "GSM2531256", "GSM2531257", "GSM2531258", "GSM2531259", "GSM2531260", "GSM2531261", "GSM2531262", "GSM2531263", "GSM2531264", "GSM2531265", "GSM2531266", "GSM2531267", "GSM2531268", "GSM2531269", "GSM2531270", "GSM2531271", "GSM2531272", "GSM2531273", "GSM2531274", "GSM2531275", "GSM2531276", "GSM2531277", "GSM2531278", "GSM2531279", "GSM2531280", "GSM2531281", "GSM2531282", "GSM2531283", "GSM2531284", "GSM2531285", "GSM2531286", "GSM2531287", "GSM2531288", "GSM2531289", "GSM2531290", "GSM2531291", "GSM2531292", "GSM2531293", "GSM2531294", "GSM2531295", "GSM2531296", "GSM2531297", "GSM2531298", "GSM2531299", "GSM2531300", "GSM2531301", "GSM2531302", "GSM2531303", "GSM2531304", "GSM2531305", "GSM2531306", "GSM2531307", "GSM2531308", "GSM2531309", "GSM2531310", "GSM2531311", "GSM2531312", "GSM2531313", "GSM2531314", "GSM2531315", "GSM2531316", "GSM2531317", "GSM2531318", "GSM2531319", "GSM2531320", "GSM2531321", "GSM2531322", "GSM2531323", "GSM2531324", "GSM2531325", "GSM2531326", "GSM2531327", "GSM2531328", "GSM2531329", "GSM2531330", "GSM2531331", "GSM2531332", "GSM2531333", "GSM2531334", 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To evaluate differentially expressed protein-coding genes meaningfully associated with BC prognosis and to obtain a better-fitting model, we used LASSO to downscale the high-dimensional information by adding a constraint to the absolute value of the coefficients and further screening by 10-fold cross-validation. 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Results: We found prognostic models consist"], "order_basis": "paper_text_order:760", "id": "assay:3"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ined in RPMI-1640 medium and MDA-MB-231 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (G", "hermo Fisher Scientific, Inc.). SK-BR-3 were cultured in DMEM complete medium (iCell-h189-001b). Huma", "rom iCell Bioscience Inc, China. MCF-10A was cultured in special medium (iCell-h131-001b). Vemurafeni", "als and methods Cell culture Human BC lines (MCF-7, MDA-MB-231, SK-BR-3) were obtained from the"], "order_basis": "paper_text_order:1985", "id": "assay:4"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Univariate Cox analysis was performed on 181 differentially expressed protein coding genes associated with immune escape in BC, and 31 of them were identified to be associated with survival ( Figure 3A ). To evaluate differentially expressed protein-coding genes meaningfully associated with BC prognosis and to obtain a better-fitting model, we used LASSO to downscale the high-dimensional information by adding a constraint to the absolute value of the coefficients and further screening by 10-fold cross-validation. The optimal λ value (λmin=0.025) was obtained from the minimum local likelihood deviation, and 16 genes were found to be significantly associated with prognosis ( Figures 3B, C )."], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}