Comparative Proteomics Analysis of Growth-Primed Adult Dorsal Root Ganglia Reveals Key Molecular Mediators for Peripheral Nerve Regeneration.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36526365", "kind": "science-record", "revision": 4, "content_hash": "sha256:96c81a13a63bb2754ba72ee3acfcb216e0ed7b833d3cc69988dfa1b886e8122f", "created_at": "2026-11-01T19:01:02Z", "updated_at": "2026-11-01T22:50:51Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Comparative Proteomics Analysis of Growth-Primed Adult Dorsal Root Ganglia Reveals Key Molecular Mediators for Peripheral Nerve Regeneration.", "doi": "10.1523/ENEURO.0168-22.2022", "pmid": "36526365", "authors": ["Maricris Bautista", "George S Katselis", "Bari Chowdhury", "Paulos Chumala", "Ruhi Mahendra", "Priyanshi Desai", "Justin Hall", "Subha Kalyaanamoorthy", "Anand Krishnan"], "journal": "eNeuro", "year": 2023, "abstract": "Injuries to peripheral nerves are frequent, yet no drug therapies are available for effective nerve repair. The slow growth rate of axons and inadequate access to growth factors challenge natural repair of nerves. A better understanding of the molecules that can promote the rate of axon growth may reveal therapeutic opportunities. Molecular profiling of injured neurons at early intervals of injury, when regeneration is at the maximum, has been the gold standard for exploring growth promoters. A complementary in vitro regenerative priming model was recently shown to induce enhanced outgrowth in adult sensory neurons. In this work, we exploited the in vitro priming model to reveal novel candidates for adult nerve regeneration. We performed a whole-tissue proteomics analysis of the in vitro primed dorsal root ganglia (DRGs) from adult SD rats and compared their molecular profile with that of the in vivo primed, and control DRGs. The proteomics data generated are available via ProteomeXchange with identifier PXD031927. From the follow-up analysis, Bioinformatics interventions, and literature curation, we identified several molecules that were differentially expressed in the primed DRGs with a potential to modulate adult nerve regrowth. We then validated the growth promoting roles of mesencephalic astrocyte-derived neurotrophic factor (MANF), one of the hits we identified, in adult rat sensory neurons. Overall, in this study, we explored two growth priming paradigm and shortlisted several candidates, and validated MANF, as potential targets for adult nerve regeneration. We also demonstrate that the in vitro priming model is a valid tool for adult nerve regeneration studies.", "claims": [{"id": "claim-method", "statement": "Mass Profiler Professional (MPP, version 15.0, Agilent Technologies) software was used for the statistical analysis (one-way ANOVA). A cutoff value of p < 0.05 and the Benjamini and Hochberg FDR set at <1% were used to obtain statistically significant results.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36526365", "locator": "sections[5] \"Whole-tissue proteomics studies\" paragraph 3", "quote": "Mass Profiler Professional (MPP, version 15.0, Agilent Technologies) software was used for the statistical analysis (one-way ANOVA). A cutoff value of p < 0.05 and the Benjamini and Hochberg FDR set at <1% were used to obtain statistically significant results."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data are deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD031927 (Perez-Riverol et al., 2022).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36526365", "locator": "sections[5] \"Whole-tissue proteomics studies\" paragraph 3", "quote": "The mass spectrometry proteomics data are deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD031927 (Perez-Riverol et al., 2022)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study validated the growth-promoting role of MANF in adult rat sensory neurons and showed that exogenous MANF improves neurite outgrowth, branch length, and branch number.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36526365", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We then validated the growth promoting roles of mesencephalic astrocyte-derived neurotrophic factor (MANF), one of the hits we identified, in adult rat sensory neurons."}, {"asset": "source:pmid:36526365", "locator": "sections[16] \"Validation of the expression and growth promoting role of MANF in adult PNS neurons\" paragraph 2", "quote": "MANF also improved the number of branches and branch length in adult neurons substantiating its growth promoting roles in the PNS (Fig. 7E,F)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study validated the growth-promoting role of MANF in adult rat sensory neurons and showed that exogenous MANF improves neurite outgrowth, branch length, and branch number.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36526365"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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