Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36531950", "kind": "science-record", "revision": 3, "content_hash": "sha256:23b7985ecdc44ddf9a0414012b5a7bf89e1182176be4d59eff9cecc51773ddee", "created_at": "2026-11-01T19:01:03Z", "updated_at": "2026-11-01T22:08:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Upregulated ENC1 predicts unfavorable prognosis and correlates with immune infiltration in endometrial cancer.", "doi": "10.3389/fcell.2022.919637", "pmid": "36531950", "authors": ["Lingling He", "Wenjing He", "Ji Luo", "Minjuan Xu"], "journal": "Frontiers in cell and developmental biology", "year": 2022, "abstract": "A better knowledge of the molecular process behind uterine corpus endometrial carcinoma (UCEC) is important for prognosis prediction and the development of innovative targeted gene therapies. The purpose of this research is to discover critical genes associated with UCEC. We analyzed the gene expression profiles of TCGA-UCEC and GSE17025, respectively, using Weighted Gene Co-expression Network Analysis (WGCNA) and differential gene expression analysis. From four sets of findings, a total of 95 overlapping genes were retrieved. On the 95 overlapping genes, KEGG pathway and GO enrichment analysis were conducted. Then, we mapped the PPI network of 95 overlapping genes using the STRING database. Twenty hub genes were evaluated using the Cytohubba plugin, including NR3C1, ATF3, KLF15, THRA, NR4A1, FOSB, PER3, HLF, NTRK3, EGR3, MAPK13, ARNTL2, PKM2, SCD, EIF5A, ADHFE1, RERGL, TUB, and ENC1. The expression levels of NR3C1, PKM2, and ENC1 were shown to be adversely linked with the survival time of UCEC patients using univariate Cox regression analysis and Kaplan-Meier survival calculation. ENC1 were also overexpressed in UCEC tumor tissues or cell lines, as shown by quantitative real-time PCR and Western blotting. Then we looked into it further and discovered that ENC1 expression was linked to tumor microenvironment and predicted various immunological checkpoints. In conclusion, our data indicate that ENC1 may be required for the development of UCEC and may serve as a future biomarker for diagnosis and therapy.", "claims": [{"id": "claim-method", "statement": "Using data from the TCGA database, a Kaplan–Meier univariate survival analysis was used to investigate the connection between overall survival (OS) and hub genes in patients.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36531950", "locator": "sections[6] \"Assessing transcript levels and prognostic value of hub genes\" paragraph 2", "quote": "Using data from the TCGA database, a Kaplan–Meier univariate survival analysis was used to investigate the connection between overall survival (OS) and hub genes in patients."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36531950", "locator": "sections[22] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Increased ENC1 expression was associated with poorer overall survival in UCEC patients, and ENC1 expression was associated with immune-cell infiltration and immunological checkpoints.", "kind": "quantitative", "target": {"value": 0.05, "unit": "p-value", "tolerance": null, "source": "According to Kaplan–Meier analyses, increased expression of three genes was significantly related to poorer overall survival in UCEC patients (p < 0.05)."}, "evidence": [{"asset": "source:pmid:36531950", "locator": "sections[17] \"Transcript levels of hub genes and prognosis in uterine corpus endometrial carcinoma\" paragraph 2", "quote": "According to Kaplan–Meier analyses, increased expression of three genes was significantly related to poorer overall survival in UCEC patients (p < 0.05)."}, {"asset": "source:pmid:36531950", "locator": "sections[19] \"The association between ENC1 and tumor immune microenvironment\" paragraph 2", "quote": "Based on the above, we found that the expression of ENC1 in UCEC may influence the immune infiltration."}, {"asset": "source:pmid:36531950", "locator": "sections[19] \"The association between ENC1 and tumor immune microenvironment\" paragraph 2", "quote": "Using the TIMER database, we discovered a negative link between ENC1 and a subset of immunological checkpoints (CD244 and CTLA4) but a positive correlation between ENC1 and other checkpoints (CD274 and CD276)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Increased ENC1 expression was associated with poorer overall survival in UCEC patients, and ENC1 expression was associated with immune-cell infiltration and immunological checkpoints.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36531950"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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All cell lines were cultured in a humidified 37°C incubator with 5% CO2.", "Academy of Sciences. All the cell lines were cultured in DMEM supplemented with 10% fetal bovine seru", "(Gibco, United States). All cell lines were cultured in a humidified 37°C incubator with 5% CO2. Col"], "order_basis": "paper_text_order:1806", "id": "assay:3"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["survival_km"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["Kaplan-Meier"], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}