Analysis of co-expression gene network associated with intracranial aneurysm and type 2 diabetes mellitus.

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Microarray datasets (GSE55650, GSE25462, GSE26969, GSE75436, and GSE13353) from the GEO database were analyzed in this research. Screening of the IA and the T2DM datasets yielded a total of 126 DEGs, among which 78 were upregulated and 138 were downregulated. Functional enrichment analysis revealed that these DEGs were enriched for a total of 68 GO pathways, including extracellular matrix composition, coagulation regulation, hemostasis regulation, and collagen fiber composition pathways. We also constructed transcriptional regulatory networks, and identified key transcription factors involved in both the conditions. Univariate logistic regression analysis showed that ARNTL2 and STAT1 were significantly associated with the development of T2DM and IA, acting as the common neurovascular markers for both the diseases. In cellular experiments, hyperglycemic microenvironments exhibited upregulated STAT1 expression. STAT1 may be involved in the pathogenesis of IA in T2DM patients. Being the common neurovascular markers, STAT1 may acts as novel therapeutic targets for the treatment of IA and T2DM.", "claims": [{"id": "claim-method", "statement": "We further explored the potential clinical diagnostic value of these seven TFs (STAT1, RELA, NFKB1, SP1, STAT3, SP3, and TP53) by plotting their ROC curves based on univariate logistic regression in the diagnosis of the two diseases (Figures 3D–J).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36561297", "locator": "sections[15] \"Construction of target genes-related transcriptional regulatory networks and identification of key TFs\" paragraph 2", "quote": "We further explored the potential clinical diagnostic value of these seven TFs (STAT1, RELA, NFKB1, SP1, STAT3, SP3, and TP53) by plotting their ROC curves based on univariate logistic regression in the diagnosis of the two diseases (Figures 3D–J)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author/s.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36561297", "locator": "sections[21] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author/s."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The results showed that the AUC values of STAT1 in both diseases were >0.8 indicating that it was significantly associated with the occurrence of both the diseases.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36561297", "locator": "sections[15] \"Construction of target genes-related transcriptional regulatory networks and identification of key TFs\" paragraph 2", "quote": "The results showed that the AUC values of STAT1 in both diseases were >0.8 indicating that it was significantly associated with the occurrence of both the diseases."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The results showed that the AUC values of STAT1 in both diseases were >0.8 indicating that it was significantly associated with the occurrence of both the diseases.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36561297"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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