In vivo localization of iron starvation induced proteins under variable iron supplementation regimes in Phaeodactylum tricornutum.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36582220", "kind": "science-record", "revision": 4, "content_hash": "sha256:7648ba94e4ddf03e42ce44c4c3e5cbbb8daaad41e13992edd89571a3ce9644a0", "created_at": "2026-11-01T19:01:08Z", "updated_at": "2026-11-01T22:50:58Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "In vivo localization of iron starvation induced proteins under variable iron supplementation regimes in Phaeodactylum tricornutum.", "doi": "10.1002/pld3.472", "pmid": "36582220", "authors": ["Elena Kazamia", "Jan Mach", "Jeffrey B McQuaid", "Xia Gao", "Tyler H Coale", "Ronald Malych", "Jean-Michel Camadro", "Emmanuel Lesuisse", "Andrew E Allen", "Chris Bowler", "Robert Sutak"], "journal": "Plant direct", "year": 2022, "abstract": "The model pennate diatom Phaeodactylum tricornutum is able to assimilate a range of iron sources. It therefore provides a platform to study different mechanisms of iron processing concomitantly in the same cell. In this study, we follow the localization of three iron starvation induced proteins (ISIPs) in vivo, driven by their native promoters and tagged by fluorophores in an engineered line of P. tricornutum. We find that the localization patterns of ISIPs are dynamic and variable depending on the overall iron status of the cell and the source of iron it is exposed to. Notwithstanding, a shared destination of the three ISIPs both under ferric iron and siderophore-bound iron supplementation is a globular compartment in the vicinity of the chloroplast. In a proteomic analysis, we identify that the cell engages endocytosis machinery involved in the vesicular trafficking as a response to siderophore molecules, even when these are not bound to iron. Our results suggest that there may be a direct vesicle traffic connection between the diatom cell membrane and the periplastidial compartment (PPC) that co-opts clathrin-mediated endocytosis and the \"cytoplasm to vacuole\" (Cvt) pathway, for proteins involved in iron assimilation. Proteomics data are available via ProteomeXchange with identifier PXD021172.\nHighlight: The marine diatom P. tricornutum engages a vesicular network to traffic siderophores and phytotransferrin from the cell membrane directly to a putative iron processing site in the vicinity of the chloroplast.", "claims": [{"id": "claim-method", "statement": "Table S3 describes the top 100 proteins that showed either an upregulated or repressed response under the two conditions ranked by the p values of an analysis of variance (see Section 2 for more details).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36582220", "locator": "sections[19] \"Proteomics analysis of the response of P. tricornutum to iron‐free siderophore stimulation\" paragraph 3", "quote": "Table S3 describes the top 100 proteins that showed either an upregulated or repressed response under the two conditions ranked by the p values of an analysis of variance (see Section 2 for more details)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data generated for this study are available in the Supporting Information. 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Proteomics data are available via ProteomeXchange with identifier PXD021172."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In a proteomic analysis, we identify that the cell engages endocytosis machinery involved in the vesicular trafficking as a response to siderophore molecules, even when these are not bound to iron.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36582220", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "In a proteomic analysis, we identify that the cell engages endocytosis machinery involved in the vesicular trafficking as a response to siderophore molecules, even when these are not bound to iron."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "In a proteomic analysis, we identify that the cell engages endocytosis machinery involved in the vesicular trafficking as a response to siderophore molecules, even when these are not bound to iron.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36582220"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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