The involvement of the primo vascular system in local enteritis and its modification by electroacupuncture.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36713388", "kind": "science-record", "revision": 2, "content_hash": "sha256:77779b2bba98e1384fc2da744010b480bc99ddb82ea77119a6bf22fc1cc0bea0", "created_at": "2026-11-01T19:01:16Z", "updated_at": "2026-11-01T21:29:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The involvement of the primo vascular system in local enteritis and its modification by electroacupuncture.", "doi": "10.3389/fimmu.2022.1072996", "pmid": "36713388", "authors": ["Sha Nan", "Juan Wan", "Qianghui Lei", "Xinya Wang", "Ning Ma", "Ruiling Yin", "Jiandi Zhu", "Mingxing Ding", "Yi Ding"], "journal": "Frontiers in immunology", "year": 2022, "abstract": "Introduction: The primo vascular system (PVS), an intensive network structure, has been claimed to be representative of the acupuncture meridian. Here, we explored the role of the PVS in local enteritis and its modification by acupuncture.\nMethods: Chronic cecitis in rabbits was induced by 2,4,6-trinitro-benzene-sulfonic acid (TNBS). The PVS on the cecum was visualized with trypan blue staining, and collected with the help of microsurgical forceps under an optical stereomicroscope.\nResults: The increased primo vessels (PVs) and primo nodes (PNs) of the PVS on the surface of the cecum were induced by local inflammation, which was positively correlated with the inflammatory cells in the cecal mucosa. Tandem mass tag (TMT) based proteomic analysis revealed that 110 differentiated proteins of the PVS existed between TNBS-treated and control rabbits; 65 proteins were upregulated, while 45 proteins were downregulated. These proteins were mainly enriched in inflammation- and immunity-related processes, such as inflammatory cell proliferation, antigen presentation, and cell adhesion in the proliferated PVS (data are available via ProteomeXchange with the identifiers PXD034280). Importantly, TNBS-induced cecitis, the proliferated PVS and inflammation response-related proteins (CD40, CD45, HLA-DRA1, LAMP1, JAGN1 and FGL1) in the PVS were alleviated or reversed by repetitive electroacupuncture (EA) stimulations.\nConclusion: These results suggest that the proliferated PVS and its active inclusions were related to the inflammatory process, which was modified by EA. Our study provides a new avenue for further exploration of the mechanism by which EA exerts anti-inflammatory effects.", "claims": [{"id": "claim-method", "statement": "The Mann–Whitney U-test (unpaired) was used to analyze the inflammatory scores of the cecum and the number of PVS. A one-way analysis of repeated variance, followed by Bonferroni’s post hoc test, was performed to assess the expression levels of TNF-α, IL-6, and MPO, as well as the changes in VMR to CRD between the different groups. An unpaired Student’s t test was used to compared the expression in the differential proteins of the PVS between the control and TNBS groups. Spearman correlation was applied to analyze the relevance between the number of PVS on the surface of the cecum and the number of inflammatory cells in the cecal mucosa using Statistical Product Service Solutions 21.0 software (SPSS Inc., USA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36713388", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "The Mann–Whitney U-test (unpaired) was used to analyze the inflammatory scores of the cecum and the number of PVS. A one-way analysis of repeated variance, followed by Bonferroni’s post hoc test, was performed to assess the expression levels of TNF-α, IL-6, and MPO, as well as the changes in VMR to CRD between the different groups. An unpaired Student’s t test was used to compared the expression in the differential proteins of the PVS between the control and TNBS groups. Spearman correlation was applied to analyze the relevance between the number of PVS on the surface of the cecum and the number of inflammatory cells in the cecal mucosa using Statistical Product Service Solutions 21.0 software (SPSS Inc., USA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: http://www.proteomexchange.org/, PXD034280.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36713388", "locator": "sections[18] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. 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