Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36718241", "kind": "science-record", "revision": 3, "content_hash": "sha256:de3f45dc383902694b8e8c3c9404b773804eaf165400c7f1a9dffc8aa7d534ce", "created_at": "2026-11-01T19:01:17Z", "updated_at": "2026-11-01T22:08:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Upregulation of miR-142 in papillary thyroid carcinoma tissues: a report based on in silico and in vitro analysis.", "doi": "10.22099/mbrc.2022.43947.1757", "pmid": "36718241", "authors": ["Sepehr Valizadeh", "Mojtaba Zehtabi", "Neda Feiziordaklou", "Zahra Akbarpour", "Amir Mahdi Khamaneh", "Mortaza Raeisi"], "journal": "Molecular biology research communications", "year": 2022, "abstract": "Papillary thyroid carcinoma (PTC) accounts for approximately 80% of all human thyroid malignancies. Recently, there has been a dramatic rise in the prevalence of thyroid cancer all over the globe. Through analysis of the GEO database, GSE104005, the authors of the current research were able to determine the differential expression of microRNAs (DEMs) as well as their target genes. Real-time PCR was used on a total of 40 samples, 40 of which were from PTC samples and 40 from normal tissues, in order to validate the discovered DEMs and the genes. Gene Ontology (GO) categories were identified, and KEGG was used to conduct pathway enrichment analysis. The multiMiR R package was used to predict target genes of DEMs. Mir-142 was found to be overexpressed in PTC samples, as compared to normal tissues, and this was validated by the identification and validation. In addition, metal ion binding and the cellular response to metal ions were identified as essential pathways in the carcinogenesis of PTC. This demonstrates their significance in the development of this malignancy. The results of our research will serve as the foundation for further research in the area of miRNA-based cancer treatment.", "claims": [{"id": "claim-method", "statement": "A paired t-test was carried out since the data distribution for Rad17 and Rrm2b, as well as miR-142 and miR-420, were all normal.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36718241", "locator": "sections[2] \"MATERIALS AND METHODS\" paragraph 12", "quote": "A paired t-test was carried out since the data distribution for Rad17 and Rrm2b, as well as miR-142 and miR-420, were all normal."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The microRNA expression profile known as GSE104005 was obtained by accessing the Gene Expression Omnibus (GEO) database at the following address: https://www.ncbi.nlm.nih.gov/geo/ [11].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36718241", "locator": "sections[2] \"MATERIALS AND METHODS\" paragraph 2", "quote": "The microRNA expression profile known as GSE104005 was obtained by accessing the Gene Expression Omnibus (GEO) database at the following address: https://www.ncbi.nlm.nih.gov/geo/ [11]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "miR-142 expression was 2.76-fold higher in PTC tissues than in normal adjacent tissues, with a statistically significant p-value of 0.049.", "kind": "quantitative", "target": {"value": 2.76, "unit": "fold change", "tolerance": null, "source": "In contrast to the normal tissues that were adjacent to the PTC, the expression level of miR-142 was found to be 2.76 times higher in the PTC tissues. This result, which was statistically significant with a p-value of 0.049, is shown in figure 2."}, "evidence": [{"asset": "source:pmid:36718241", "locator": "sections[3] \"RESULTS\" paragraph 8", "quote": "In contrast to the normal tissues that were adjacent to the PTC, the expression level of miR-142 was found to be 2.76 times higher in the PTC tissues. This result, which was statistically significant with a p-value of 0.049, is shown in figure 2."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "miR-142 expression was 2.76-fold higher in PTC tissues than in normal adjacent tissues, with a statistically significant p-value of 0.049.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36718241"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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