IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36732736", "kind": "science-record", "revision": 3, "content_hash": "sha256:1b9e18d86f12ab6c22f61e1fd52f2c9ffe35b0f199909e8611ff138e16bd04b5", "created_at": "2026-11-01T19:01:17Z", "updated_at": "2026-11-01T22:08:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer.", "doi": "10.1186/s12885-022-10353-5", "pmid": "36732736", "authors": ["Wei Huang", "Lizhen Zhu", "Haoxuan Huang", "Yuanyuan Li", "Gongxian Wang", "Cheng Zhang"], "journal": "BMC cancer", "year": 2023, "abstract": "BACKGROUND: IGF2BP3 expression is associated with poor prognosis in cancers of multiple tissue origins. However, the precise mechanism of its co-carcinogenic action in bladder cancer is unknown.\nMETHODS: We aimed to demonstrate the relationship between IGF2BP3 expression and pan-cancer using The Cancer Genome Atlas (TCGA) database. We next validated IGF2BP3 expression in the Gene Expression Omnibus (GEO) database (GSE3167). Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic values of IGF2BP3. Cox and logistic regression were used to explore the factors affecting the prognosis. Protein-protein interactions (PPIs) network was constructed by STRING. Enrichment analyses were performed to infer involved pathways and functional categories of IGF2BP3 using the cluster Profiler package. We applied single-sample gene set enrichment analysis (ssGSEA) algorithm and TIMER database to evaluate the expression level of immune genes.\nRESULTS: Pan-cancer analyses reveal that IGF2BP3 was higher in most cancer types, including bladder cancer, and the same results were found in GSE3167. The area under the ROC curve of IGF2BP3 was 0.736, which indicated that IGF2BP3 may be a potential diagnostic biomarker. High IGF2BP3 expression was associated with poorer overall survival (OS) (P = 0.015). For validation, we collected 95 bladder cancer samples and found that IGF2BP3 expression was higher in bladder cancer tissues than that in non-tumor bladder tissues by immunohistochemistry staining. We found a positive correlation between the expression level of IGF2BP3 and the clinical stage of bladder cancer. Immunocyte infiltration analysis showed that high IGF2BP3 expression was correlated with regulating the infiltration level of immune cell, including neutrophil cells and macrophages. IGF2BP3 promotes migration and invasion of bladder cancer cells, while IGF2BP3 inhibition had the opposite effects. Higher IGF2BP3 expression was closely associated with advanced TNM stage.\nCONCLUSION: IGF2BP3 overexpression was related to disease progression and poor prognosis, as well as infiltration of immune cells in bladder cancer. IGF2BP3 can be a promising independent prognostic biomarker and potential treatment target for bladder cancer.", "claims": [{"id": "claim-method", "statement": "To better understand the potential risk factors associated with the overall survival of patients, we performed univariate and multivariate analyses by the Cox regression model (Fig. 4A-B).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36732736", "locator": "sections[16] \"High IGF2BP3 expression predicts a poor prognosis in bladder cancer\" paragraph 2", "quote": "To better understand the potential risk factors associated with the overall survival of patients, we performed univariate and multivariate analyses by the Cox regression model (Fig. 4A-B)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets of this study are available on request to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36732736", "locator": "sections[30] \"Availability of data and materials\" paragraph 2", "quote": "The datasets of this study are available on request to the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High IGF2BP3 expression was associated with poorer overall survival in bladder cancer.", "kind": "quantitative", "target": {"value": 95.0, "unit": "per cent", "tolerance": null, "source": "Kaplan–Meier survival curves showed that patients with poorer survival had higher levels of IGF2BP3 mRNA expression (HR = 1.44, 95% CI: 1.07–1.94, P = 0.015) (Fig. 4C)."}, "evidence": [{"asset": "source:pmid:36732736", "locator": "sections[16] \"High IGF2BP3 expression predicts a poor prognosis in bladder cancer\" paragraph 2", "quote": "Kaplan–Meier survival curves showed that patients with poorer survival had higher levels of IGF2BP3 mRNA expression (HR = 1.44, 95% CI: 1.07–1.94, P = 0.015) (Fig. 4C)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "High IGF2BP3 expression was associated with poorer overall survival in bladder cancer.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36732736"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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We found a positive correlation be", "d CD3 (Figure D) in bladdercancer tissues by immunohistochemistry. Figure S4. IGF2BP3 expression was significa", "ith CD274, CD68, CD16, and CD3 expression by immunohistochemistry.Quantification of the average optical densit"], "order_basis": "paper_text_order:1560", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["my of Sciences (Shanghai, China). 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