Enzymatic and proteomic exploration into the inhibitory activities of lemongrass and lemon essential oils against Botrytis cinerea (causative pathogen of gray mold).

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However, the underpinning enzymatic and proteomic mechanism for these inhibitory effects is not entirely clear.\nMethods: Thus, this study examined the effects of lemon (Le) and lemongrass (Lg) EOs (individually and in combination) against B. cinerea based on enzymatic and proteomic analyses. Proteomics data are available via ProteomeXchange with identifier PXD038894.\nResults and discussion: Both EOs (individually and in combination) displayed abilities to induce scavenging as observed with the reduction of H2O2. Measured malondialdehyde (MDA) and superoxide dismutase (SOD) activity were increased in all EOs treated B. cinerea mycelia compared to the control. Ascorbate peroxidase (APX) activity was highest in Lg treated B. cinerea (206% increase), followed by combined (Le + Lg) treatment with 73% compared to the untreated control. Based on GC-MS analysis, the number of volatile compounds identified in lemon and lemongrass EOs were 7 and 10, respectively. Major chemical constituent of lemon EO was d-limonene (71%), while lemongrass EO was a-citral (50.1%). Based on the interrogated LC-MS data, 42 distinct proteins were identified, and 13 of these proteins were unique with 1, 8, and 4 found in Le-, Lg-, and (Le + Lg) EOs treated B. cinerea, respectively, and none in control. Overall, 72% of identified proteins were localized within cellular anatomical entity, and 28% in protein-complexes. Proteins involved in translation initiation, antioxidant activity, protein macromolecule adaptor activity and microtubule motor activity were only identified in the Lg and (Le + Lg) EOs treated B. cinerea mycelia, which was consistent with their APX activities.", "claims": [{"id": "claim-method", "statement": "Peptide and Protein Prophet algorithms were used for peptide and protein validation, and the quantitation of protein was performed using Fischer’s Exact Test on the paired data with the Benjamini-Hochberg correction applied.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36741895", "locator": "sections[16] \"Quality control and protein quantification\" paragraph 2", "quote": "Peptide and Protein Prophet algorithms were used for peptide and protein validation, and the quantitation of protein was performed using Fischer’s Exact Test on the paired data with the Benjamini-Hochberg correction applied."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The proteomics data presented in the study are deposited to the ProteomeXchange Consortium via the PRIDE partner repository (doi: 10.6019/PXD038894) with the dataset identifier PXD038894.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36741895", "locator": "sections[32] \"Data availability statement\" paragraph 2", "quote": "The proteomics data presented in the study are deposited to the ProteomeXchange Consortium via the PRIDE partner repository (doi: 10.6019/PXD038894) with the dataset identifier PXD038894."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Based on the interrogated LC-MS data, 42 distinct proteins were identified, and 13 of these proteins were unique with 1, 8, and 4 found in Le-, Lg-, and (Le + Lg) EOs treated B. cinerea, respectively, and none in control.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36741895", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Based on the interrogated LC-MS data, 42 distinct proteins were identified, and 13 of these proteins were unique with 1, 8, and 4 found in Le-, Lg-, and (Le + Lg) EOs treated B. cinerea, respectively, and none in control."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Based on the interrogated LC-MS data, 42 distinct proteins were identified, and 13 of these proteins were unique with 1, 8, and 4 found in Le-, Lg-, and (Le + Lg) EOs treated B. cinerea, respectively, and none in control.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36741895"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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