The ratio of adaptive to innate immune cells differs between genders and associates with improved prognosis and response to immunotherapy.

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However, not all cancer patients benefit, and current stratification strategies based primarily on PD1 status and mutation burden are far from perfect. We hypothesised that high activation of an innate response relative to the adaptive response may prevent proper tumour neoantigen identification and decrease the specific anticancer response, both in the presence and absence of immunotherapy. To investigate this, we obtained transcriptomic data from three large publicly available cancer datasets, the Cancer Genome Atlas (TCGA), the Hartwig Medical Foundation (HMF), and a recently published cohort of metastatic bladder cancer patients treated with immunotherapy. To analyse immune infiltration into bulk tumours, we developed an RNAseq-based model based on previously published definitions to estimate the overall level of infiltrating innate and adaptive immune cells from bulk tumour RNAseq data. From these, the adaptive-to-innate immune ratio (A/I ratio) was defined. A meta-analysis of 32 cancer types from TCGA overall showed improved overall survival in patients with an A/I ratio above median (Hazard ratio (HR) females 0.73, HR males 0.86, P < 0.05). Of particular interest, we found that the association was different for males and females for eight cancer types, demonstrating a gender bias in the relative balance of the infiltration of innate and adaptive immune cells. For patients with metastatic disease, we found that responders to immunotherapy had a significantly higher A/I ratio than non-responders in HMF (P = 0.036) and a significantly higher ratio in complete responders in a separate metastatic bladder cancer dataset (P = 0.022). Overall, the adaptive-to-innate immune ratio seems to define separate states of immune activation, likely linked to fundamental immunological reactions to cancer. This ratio was associated with improved prognosis and improved response to immunotherapy, demonstrating potential relevance to patient stratification. Furthermore, by demonstrating a significant difference between males and females that associates with response, we highlight an important gender bias which likely has direct clinical relevance.", "claims": [{"id": "claim-method", "statement": "Survival analyses were performed by Cox proportional hazard regression [33] and Kaplan-Meier curves.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36745657", "locator": "sections[7] \"Statistical analyses\" paragraph 2", "quote": "Survival analyses were performed by Cox proportional hazard regression [33] and Kaplan-Meier curves."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data are available from the Hartwig Medical Foundation and an application to access the data can be sent here https://www.hartwigmedicalfoundation.nl/en/data/data-acces-request/ for researchers who meet the criteria for access to confidential data. 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