Cuproptosis-related gene FDX1 as a prognostic biomarker for kidney renal clear cell carcinoma correlates with immune checkpoints and immune cell infiltration.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36755576", "kind": "science-record", "revision": 2, "content_hash": "sha256:4162be5b85f3e6dc977d1f3204b76a724bb2de61101033d136b7b99c54793c80", "created_at": "2026-11-01T19:01:19Z", "updated_at": "2026-11-01T21:30:04Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Cuproptosis-related gene FDX1 as a prognostic biomarker for kidney renal clear cell carcinoma correlates with immune checkpoints and immune cell infiltration.", "doi": "10.3389/fgene.2023.1071694", "pmid": "36755576", "authors": ["Yimin Yao", "Haixin Chen", "Minjun Lou", "Tingting Chen"], "journal": "Frontiers in genetics", "year": 2023, "abstract": "Background: Kidney renal clear cell carcinoma (KIRC) is not sensitive to radiotherapy and chemotherapy, and only some KIRC patients can benefit from immunotherapy and targeted therapy. Cuproptosis is a new mechanism of cell death, which is closely related to tumor progression, prognosis and immunity. The identification of prognostic markers related to cuproptosis in KIRC may provide targets for treatment and improve the prognosis of KIRC patients. Methods: Ten cuproptosis-related genes were analyzed for differential expression in KIRC-TCGA and a prognostic model was constructed. Nomogram diagnostic model was used to screen independent prognostic molecules. The screened molecules were verified in multiple datasets (GSE36895 and GSE53757), and in KIRC tumor tissues by RT-PCR and immunohistochemistry (IHC). Clinical correlation of cuproptosis-related independent prognostic molecules was analyzed. According to the molecular expression, the two groups were divided into high and low expression groups, and the differences of immune checkpoint and tumor infiltrating lymphocytes (TILs) between the two groups were compared by EPIC algorithm. The potential Immune checkpoint blocking (ICB) response of high and low expression groups was predicted by the \"TIDE\" algorithm. Results: FDX1 and DLAT were protective factors, while CDKN2A was a risk factor. FDX1 was an independent prognostic molecule by Nomogram, and low expressed in tumor tissues compared with adjacent tissues (p < 0.05). FDX1 was positively correlated with CD274, HAVCR2, PDCD1LG2, and negatively correlated with CTLA4, LAG3, and PDCD1. The TIDE score of low-FDX1 group was higher than that of high-FDX1 group. The abundance of CD4+ T cells, CD8+ T cells and Endothelial cells in FDX1-low group was lower than that in FDX1-high group (p < 0.05). Conclusion: FDX1, as a key cuproptosis-related gene, was also an independent prognostic molecule of KIRC. FDX1 might become an interesting biomarker and potential therapeutic target for KIRC.", "claims": [{"id": "claim-method", "statement": "Univariate and multivariate cox regression analysis were performed to identify the proper terms to build the Nomogram.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36755576", "locator": "sections[5] \"Screening of independent prognostic molecules related to cuproptosis (nomogram prognostic model)\" paragraph 2", "quote": "Univariate and multivariate cox regression analysis were performed to identify the proper terms to build the Nomogram."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36755576", "locator": "sections[25] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author."}], "state": "complete"}, {"id": "claim-target-result", "statement": "FDX1 was identified as an independent prognostic molecule associated with cuproptosis in KIRC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36755576", "locator": "sections[15] \"FDX1 is an independent prognostic molecule associated with cuproptosis by Nomogram\" paragraph 2", "quote": "The prognosis of FDX1 showed significant differences in univariate and multivariate, which indicated that FDX1 was a variable independent of other clinical factors (Figures 2A, B)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "FDX1 was identified as an independent prognostic molecule associated with cuproptosis in KIRC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36755576"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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