Identification and Analysis of Crucial Genes in H. pylori-Associated Gastric Cancer Using an Integrated Bioinformatics Approach.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36778919", "kind": "science-record", "revision": 2, "content_hash": "sha256:70b0da6f1614ec9d43259631da24412369f6a71db5fbbd4574e0351b7ac95a32", "created_at": "2026-11-01T19:01:22Z", "updated_at": "2026-11-01T21:30:08Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification and Analysis of Crucial Genes in H. pylori-Associated Gastric Cancer Using an Integrated Bioinformatics Approach.", "doi": "10.1155/2023/8538240", "pmid": "36778919", "authors": ["Wei Ding", "Huaji Jiang", "Nianyuan Ye", "Ling Zhuang", "Zhiping Yuan", "Yulin Tan", "Wenbo Xue", "Xuezhong Xu"], "journal": "Journal of oncology", "year": 2023, "abstract": "Background: The relationship between H. pylori infection and gastric cancer (GC) has been widely studied, and H. pylori is considered as the main factor. Utilizing bioinformatics analysis, this study examined gene signatures related to progressing H. pylori-associated GC.\nMaterials and Methods: The dataset GSE13195 was chosen to search for abnormally expressed genes in H. pylori-associated GC and normal tissues. The TCGA-STAD database was chosen to verify the expression of key genes in GC and normal tissues.\nResults: In GSE13195, a total of 332 differential expression genes (DEGs) were screened. The results of weighted gene co-expression network analysis showed that the light cyan, plum2, black, and magenta4 modules were associated with stages (T3, T2, and T4), while the orangered4, salmon2, pink, and navajowhite2 modules were correlated with lymph node metastasis (N3, N2, and N0). Based on the results of DEGs and hub genes, a total of 7 key genes (ADAM28, FCER1G, MRPL14, SOSTDC1, TYROBP, C1QC, and C3) were screened out. These gene mRNA levels were able to distinguish between normal and H. pylori-associated GC tissue using receiver operating characteristic curves. After transcriptional level verification and survival analysis, ADAM28 and C1QC were excluded. An immune infiltration study revealed that key genes were involved in regulating the infiltration levels of cells associated with innate immune response, antigen presentation process, humoral immune response, or Tcell-mediated immune response. In addition, drugs targeting FCER1G and TYROBP have been approved and are under investigation.\nConclusion: Our study identified five key genes involved in H. pylori-associated GC tumorigenesis. Patients with higher levels of C3 expression had a poorer prognosis than those with lower levels. In addition, these key genes may serve as biomarkers and therapeutic targets for H. pylori-associated GC diagnosis, targeted therapy, and immunotherapy in the future.", "claims": [{"id": "claim-method", "statement": "Gene co-expression networks in H. pylori-associated GC tissues were constructed using Sangerbox Tools [16].\nReceiver operating characteristic (ROC) curves were drawn to calculate specificity and sensitivity.\nThe expression levels of key genes were correlated with the prognosis of GC patients through survival analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36778919", "locator": "sections[6] \"2.4. Screen for Tumor Progression-Related Modules and Central Genes by Weighted Gene Co-Expression Network Analysis (WGCNA)\" paragraph 2", "quote": "Gene co-expression networks in H. pylori-associated GC tissues were constructed using Sangerbox Tools [16]."}, {"asset": "source:pmid:36778919", "locator": "sections[7] \"2.5. Validation of Key Genes\" paragraph 2", "quote": "Receiver operating characteristic (ROC) curves were drawn to calculate specificity and sensitivity."}, {"asset": "source:pmid:36778919", "locator": "sections[16] \"3.6. Validation and Survival Analysis of Key Genes\" paragraph 2", "quote": "The expression levels of key genes were correlated with the prognosis of GC patients through survival analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36778919", "locator": "sections[23] \"Data Availability\" paragraph 2", "quote": "The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified five key genes involved in H. pylori-associated gastric cancer tumorigenesis, with higher C3 expression associated with poorer prognosis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36778919", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our study identified five key genes involved in H. pylori-associated GC tumorigenesis."}, {"asset": "source:pmid:36778919", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Patients with higher levels of C3 expression had a poorer prognosis than those with lower levels."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified five key genes involved in H. pylori-associated gastric cancer tumorigenesis, with higher C3 expression associated with poorer prognosis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36778919"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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