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pmid:36812155

UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications.

Mo Awchi · Pablo Sinues · Alexandre N Datta · Diego García-Gómez · Kapil Dev Singh

Journal of proteome research · 2023 doi:10.1021/acs.jproteome.2c00835

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UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications.
doi
10.1021/acs.jproteome.2c00835
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Mo Awchi
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Pablo Sinues
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Alexandre N Datta
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Diego García-Gómez
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Kapil Dev Singh
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Journal of proteome research
year
2023
abstract
Real-time breath analysis using secondary electrospray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to access the metabolic state of a person. However, it lacks the ability to unequivocally assign mass spectral features to compounds due to the absence of chromatographic separation. This can be overcomed by using exhaled breath condensate and conventional liquid chromatography-mass spectrometry (LC-MS) systems. In this study, to the best of our knowledge, we confirm for the first time the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr) previously reported to be involved in response to and side effects from antiseizure medications in exhaled breath condensate and by extension in exhaled human breath. Raw data are publicly available at MetaboLights with the accession number MTBLS6760.
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