LncRNA LINC01094 Promotes Cells Proliferation and Metastasis through the PTEN/AKT Pathway by Targeting AZGP1 in Gastric Cancer.

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Herein, through next-generation sequencing, we screened metastasis-driving molecules by using tissues from early-stage gastric cancer (GC) patients with lymph node metastasis, and we identified a lncRNA LINC01094, which was associated with the metastasis of GC. According to the clinical data from the TCGA, GSE15459, and GSE62254 cohorts, the high expression of LINC01094 was associated with an unfavorable prognosis. Moreover, 106 clinical GC and paired normal samples were collected, and the qRT-PCR results showed that the high expression of LINC01094 was associated with high T and N stages and a poor prognosis. We found that LINC01094 promotes the proliferation and metastasis of GC in vitro and in vivo. AZGP1 was found as the protein-binding partner of LINC01094 by using RNA pulldown and RNA-binding protein immunoprecipitation (RIP) assays. LINC01094 antagonizes the function of AZGP1, downregulates the expression of PTEN, and further upregulates the AKT pathway. Collectively, our results suggested that LINC01094 might predict the prognosis of GC patients and become the therapy target for GC.", "claims": [{"id": "claim-data-access", "statement": "The data sets generated and analyzed during the current study are available in the TCGA and GEO, TCGA: https://portal.gdc.cancer.gov, accessed on 28 September 2020; GEO: https://www.ncbi.nlm.nih.gov/geo, accessed on 28 September 2020. FUSCC cohort data used and analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36831602", "locator": "sections[36] \"Data Availability Statement\" paragraph 2", "quote": "The data sets generated and analyzed during the current study are available in the TCGA and GEO, TCGA: https://portal.gdc.cancer.gov, accessed on 28 September 2020; GEO: https://www.ncbi.nlm.nih.gov/geo, accessed on 28 September 2020. 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