Ferroptosis-related genes are involved in asthma and regulate the immune microenvironment.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36843917", "kind": "science-record", "revision": 3, "content_hash": "sha256:69392c37aeb242ea75aabe9c0c2331a3f56df416c658cfee118663cf275de6a9", "created_at": "2026-11-01T19:01:27Z", "updated_at": "2026-11-01T22:08:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Ferroptosis-related genes are involved in asthma and regulate the immune microenvironment.", "doi": "10.3389/fphar.2023.1087557", "pmid": "36843917", "authors": ["Haixia Wang", "Yuanmin Jia", "Junlian Gu", "Ou Chen", "Shouwei Yue"], "journal": "Frontiers in pharmacology", "year": 2023, "abstract": "Background: Asthma was a chronic inflammatory illness driven by complicated genetic regulation and environmental exposure. The complex pathophysiology of asthma has not been fully understood. Ferroptosis was involved in inflammation and infection. However, the effect of ferroptosis on asthma was still unclear. The study was designed to identify ferroptosis-related genes in asthma, providing potential therapeutic targets. Methods: We conducted a comprehensive analysis combined with WGCNA, PPI, GO, KEGG, and CIBERSORT methods to identify ferroptosis-related genes that were associated with asthma and regulated the immune microenvironment in GSE147878 from the GEO. The results of this study were validated in GSE143303 and GSE27066, and the hub genes related to ferroptosis were further verified by immunofluorescence and RT-qPCR in the OVA asthma model. Results: 60 asthmatics and 13 healthy controls were extracted for WGCNA. We found that genes in the black module (r = -0.47, p < 0.05) and magenta module (r = 0.51, p < 0.05) were associated with asthma. CAMKK2 and CISD1 were discovered to be ferroptosis-related hub genes in the black and magenta module, separately. We found that CAMKK2 and CISD1 were mainly involved in the CAMKK-AMPK signaling cascade, the adipocytokine signaling pathway, the metal cluster binding, iron-sulfur cluster binding, and 2 iron, 2 sulfur cluster binding in the enrichment analysis, which was strongly correlated with the development of ferroptosis. We found more infiltration of M2 macrophages and less Tregs infiltration in the asthma group compared to healthy controls. In addition, the expression levels of CISD1 and Tregs were negatively correlated. Through validation, we found that CAMKK2 and CISD1 expression were upregulated in the asthma group compared to the control group and would inhibit the occurrence of ferroptosis. Conclusion: CAMKK2 and CISD1 might inhibit ferroptosis and specifically regulate asthma. Moreover, CISD1 might be tied to the immunological microenvironment. Our results could be useful to provide potential immunotherapy targets and prognostic markers for asthma.", "claims": [{"id": "claim-method", "statement": "We conducted a comprehensive analysis combined with WGCNA, PPI, GO, KEGG, and CIBERSORT methods to identify ferroptosis-related genes that were associated with asthma and regulated the immune microenvironment in GSE147878 from the GEO.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36843917", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "We conducted a comprehensive analysis combined with WGCNA, PPI, GO, KEGG, and CIBERSORT methods to identify ferroptosis-related genes that were associated with asthma and regulated the immune microenvironment in GSE147878 from the GEO."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36843917", "locator": "sections[25] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "CAMKK2 and CISD1 were discovered to be ferroptosis-related hub genes in the black and magenta module, separately.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36843917", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "CAMKK2 and CISD1 were discovered to be ferroptosis-related hub genes in the black and magenta module, separately."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "CAMKK2 and CISD1 were discovered to be ferroptosis-related hub genes in the black and magenta module, separately.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36843917"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Results"], "order_basis": "paper_text_order:850", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["e further verified by immunofluorescence and RT-qPCR in the OVA asthma model. Results: 60 asthmat", "urther verified by immunofluorescence and RT-qPCR in the OVA asthma model. Results: 60 asthmat"], "order_basis": "paper_text_order:873", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["e study by Shen et al. (2003). C57BL/6J male mice were purchased from Vital River Laboratories (Bei", "m Vital River Laboratories (Beijing, China). Mice were allowed tap water and rodent chow and were m", "22°C with a 12 h light-12 h dark cycle. All mice were acclimatized for 1 week before experimentati", "r 1 week before experimentation. The C57BL/6 mice were randomly divided into 2 groups (n = 4 per gr"], "order_basis": "paper_text_order:2385", "id": "assay:3"}, {"role": "analysis", "definition_id": "network_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["PPI"], "order_basis": "consumes_earlier_output", "id": "assay:4"}]}