Inhibition of apoptosis through AKT-mTOR pathway in ovarian cancer and renal cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36849137", "kind": "science-record", "revision": 3, "content_hash": "sha256:3d0927f5dd09b5c9787aa5fa6e8b44606d313163fedecf143311c472a95d2f98", "created_at": "2026-11-01T19:01:28Z", "updated_at": "2026-11-01T22:08:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Inhibition of apoptosis through AKT-mTOR pathway in ovarian cancer and renal cancer.", "doi": "10.18632/aging.204564", "pmid": "36849137", "authors": ["Hongrun Chen", "Lianfeng Zhang", "Meini Zuo", "Xiaowen Lou", "Bin Liu", "Taozhu Fu"], "journal": "Aging", "year": 2023, "abstract": "OBJECTIVE: Ovarian cancer and renal cancer are malignant tumors; however, the relationship between TTK Protein Kinase (TTK), AKT-mTOR pathway and ovarian cancer, renal cancer remains unclear.\nMETHODS: Download GSE36668 and GSE69428 from Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was performed. Created protein-protein interaction (PPI) network. Used Gene Ontology analysis (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) for functional enrichment analysis. Gene Set Enrichment Analysis (GSEA) analysis and survival analysis were performed. Created animal model for western blot analysis. Gene Expression Profiling Interactive Analysis (GEPIA) was performed to explore the role of TTK on the overall survival of renal cancer.\nRESULTS: GO showed that DEGs were enriched in anion and small molecule binding, and DNA methylation. KEGG analysis presented that they mostly enriched in cholesterol metabolism, type 1 diabetes, sphingolipid metabolism, ABC transporters, etc., TTK, mTOR, p-mTOR, AKT, p-AKT, 4EBP1, p-4EBP1 and Bcl-2 are highly expressed in ovarian cancer, Bax, Caspase3 are lowly expressed in ovarian cancer, cell apoptosis is inhibited, leading to deterioration of ovarian cancer. Furthermore, the TTK was not only the hub biomarker of ovarian cancer, but also one significant hub gene of renal cancer, and its expression was up-regulated in the renal cancer. Compared with the renal cancer patients with low expression of TTK, the patients with high expression of TTK have the poor overall survival (P = 0.0021).\nCONCLUSION: TTK inhibits apoptosis through AKT-mTOR pathway, worsening ovarian cancer. And TTK was also one significant hub biomarker of renal cancer.", "claims": [{"id": "claim-method", "statement": "We selected the ovarian cancer survival data from the dataset GSE140082, used R software package maxstat (version:0.7–25) to calculate optimal cut-off value of RiskScore of ten core genes, best cut-off value is calculated, Survfit function of the R package survival was further used to find prognostic differences.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36849137", "locator": "sections[11] \"Survival analysis\" paragraph 2", "quote": "We selected the ovarian cancer survival data from the dataset GSE140082, used R software package maxstat (version:0.7–25) to calculate optimal cut-off value of RiskScore of ten core genes, best cut-off value is calculated, Survfit function of the R package survival was further used to find prognostic differences."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36849137", "locator": "sections[15] \"Data availability\" paragraph 2", "quote": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Renal cancer patients with high TTK expression had poorer overall survival than patients with low TTK expression (P = 0.0021).", "kind": "quantitative", "target": {"value": 0.0021, "unit": "p-value", "tolerance": null, "source": "Compared with the renal cancer patients with low expression of TTK, the patients with high expression of TTK have the poor overall survival (P = 0.0021)."}, "evidence": [{"asset": "source:pmid:36849137", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Compared with the renal cancer patients with low expression of TTK, the patients with high expression of TTK have the poor overall survival (P = 0.0021)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Renal cancer patients with high TTK expression had poorer overall survival than patients with low TTK expression (P = 0.0021).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36849137"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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