Identification of key genes and pathways associated with gender difference in osteonecrosis of the femoral head based on bioinformatics analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36856107", "kind": "science-record", "revision": 3, "content_hash": "sha256:7aea3d742b2b57f5b42e5470a2fd996c48014ab8a8f9980b71dd6366741dffc5", "created_at": "2026-11-01T19:01:29Z", "updated_at": "2026-11-01T22:08:57Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "partial", "title": "Identification of key genes and pathways associated with gender difference in osteonecrosis of the femoral head based on bioinformatics analysis.", "doi": null, "pmid": "36856107", "authors": ["Zijian Yan", "Junchang Xu", "Guihua Wu", "Yongling Zhen", "Xiaolong Liao", "Feng Zou"], "journal": "Journal of musculoskeletal & neuronal interactions", "year": 2023, "abstract": "OBJECTIVE: To identify different key genes and pathways between males and females by studying differentially expressed genes (DEGs).\nMETHODS: The gene expression data of GSE123568 were downloaded from GEO database, including osteonecrosis of the femoral head (ONFH) samples from 3 females and 7 males, and DEGs between different gender were identified with R software. Protein-protein interaction (PPI) network was constructed to further analyze the interactions between overlapping DEGs, and finally, GO, KEGG and gene set enrichment analysis (GSEA) were conducted for enrichment analysis.\nRESULTS: 131 DEGs were identified between ONFH females and ONFH males, including 76 up-regulated genes and 55 down-regulated genes. And 10 hub genes were identified in PPI network, including SLC4A1, GYPA, CXCL8, IFIT1, GBP5, IFI44, IFI44L, IFIT3, KEL and AHSP. Functional enrichment analysis revealed that these genes were mainly enriched in cGMP-PKG signaling pathway, Fatty acid degradation, Non-alcoholic fatty liver disease, Systemic lupus erythematosus, Hematopoietic cell lineage and NO-cGMP-PKG signaling.\nCONCLUSIONS: NO-cGMP-PKG signaling may play an important role in the occurrence and development of ONFH. SLC4A1, GYPA, CXCL8, GBP5 and AHSP may be key genes associated with gender difference in the progression of ONFH, which may be ideal targets or prognostic markers for the treatment of ONFH.", "claims": [{"id": "claim-method", "statement": "P values were adjusted for comparison with the false discovery rate (FDR) of Benjamini and Hochberg (BH) test[9] in the limma package[8].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36856107", "locator": "sections[4] \"Data preprocessing and identification of DEGs\" paragraph 2", "quote": "P values were adjusted for comparison with the false discovery rate (FDR) of Benjamini and Hochberg (BH) test[9] in the limma package[8]."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The gene expression data of GSE123568 were downloaded from GEO database, including osteonecrosis of the femoral head (ONFH) samples from 3 females and 7 males, and DEGs between different gender were identified with R software.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36856107", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The gene expression data of GSE123568 were downloaded from GEO database, including osteonecrosis of the femoral head (ONFH) samples from 3 females and 7 males, and DEGs between different gender were identified with R software."}], "state": "complete"}, {"id": "claim-target-result", "statement": "131 DEGs were identified between ONFH females and ONFH males, including 76 up-regulated genes and 55 down-regulated genes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36856107", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "131 DEGs were identified between ONFH females and ONFH males, including 76 up-regulated genes and 55 down-regulated genes."}], "state": "complete"}], "gaps": ["doi"]}, "experiment": {"state": "complete", "objective": "131 DEGs were identified between ONFH females and ONFH males, including 76 up-regulated genes and 55 down-regulated genes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36856107"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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