Bioinformatic Analysis of miR-200b/429 and Hub Gene Network in Cervical Cancer.

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Using publicly available miRNA expression data from TCGA and GEO followed by independent validation, we aimed to identify the association between miR-200b/429 expression and cervical cancer. miR-200b/429 cluster was significantly overexpressed in cancer samples compared to normal samples. miR-200b/429 expression did not correlate with patient survival; however, its overexpression correlated with histological type. Protein-protein interaction analysis of 90 target genes of miR-200b/429 identified EZH2, FLT1, IGF2, IRS1, JUN, KDR, SOX2, MYB, ZEB1, and TIMP2 as the top ten hub genes. PI3K-AKT and MAPK signaling pathways emerged as major target pathways of miR-200b/429 and their hub genes. Kaplan-Meier survival analysis showed the expression of seven miR-200b/429 target genes (EZH2, FLT1, IGF2, IRS1, JUN, SOX2, and TIMP2) to influence the overall survival of patients. The miR-200a-3p and miR-200b-5p could help predict cervical cancer with metastatic potential. The cancer hallmark enrichment analysis showed hub genes to promote growth, sustained proliferation, resistance to apoptosis, induction of angiogenesis, activation of invasion, and metastasis, enabling replicative immortality, evading immune destruction, and tumor-promoting inflammation. The drug-gene interaction analysis identified 182 potential drugs to interact with 27 target genes of miR-200b/429 with paclitaxel, doxorubicin, dabrafenib, bortezomib, docetaxel, ABT-199, eribulin, vorinostat, etoposide, and mitoxantrone emerging as the top ten best candidate drugs. Taken together, miR-200b/429 and associated hub genes can be helpful for prognostic application and clinical management of cervical cancer.", "claims": [{"id": "claim-method", "statement": "The prognostic significance of miR-200b/429 and hub genes was evaluated using the Kaplan–Meier survival curve with the Log-rank method KM plotter tool (Nagy et al. 2021).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36879084", "locator": "sections[6] \"Systems Biology Analysis/Downstream Analysis\" paragraph 2", "quote": "The prognostic significance of miR-200b/429 and hub genes was evaluated using the Kaplan–Meier survival curve with the Log-rank method KM plotter tool (Nagy et al. 2021)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Not Applicable.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36879084", "locator": "sections[27] \"notes\" paragraph 1", "quote": "Not Applicable."}], "state": "complete"}, {"id": "claim-target-result", "statement": "miR-200b/429 cluster was significantly overexpressed in cancer samples compared to normal samples.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36879084", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "miR-200b/429 cluster was significantly overexpressed in cancer samples compared to normal samples."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "miR-200b/429 cluster was significantly overexpressed in cancer samples compared to normal samples.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36879084"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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