Proteomics Analysis of Lymphoblastoid Cell Lines from Patients with Amyotrophic Lateral Sclerosis.

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Some of the cellular perturbations associated with ALS can be detected in peripheral cells, including lymphocytes from blood. A related cell system that is very suitable for research consists of human lymphoblastoid cell lines (LCLs), which are immortalized lymphocytes. LCLs that can be easily expanded in culture and can be maintained for long periods as stable cultures. We investigated, on a small set of LCLs, if a proteomics analysis using liquid chromatography followed by tandem mass spectrometry reveals proteins that are differentially present in ALS versus healthy controls. We found that individual proteins, the cellular and molecular pathways in which these proteins participate, are detected as differentially present in the ALS samples. Some of these proteins and pathways are already known to be perturbed in ALS, while others are new and present interest for further investigations. These observations suggest that a more detailed proteomics analysis of LCLs, using a larger number of samples, represents a promising approach for investigating ALS mechanisms and to search for therapeutic agents. Proteomics data are available via ProteomeXchange with identifier PXD040240.", "claims": [{"id": "claim-method", "statement": "Fisher’s exact test performed with the Benjamini–Hochberg adjustment for multiple testing and a threshold of p < 0.05 identified 223 proteins that had p values below the critical value reported by the test, 0.0178.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36903260", "locator": "sections[2] \"2. Results and Discussion\" paragraph 3", "quote": "Fisher’s exact test performed with the Benjamini–Hochberg adjustment for multiple testing and a threshold of p < 0.05 identified 223 proteins that had p values below the critical value reported by the test, 0.0178."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Proteomics data are available via ProteomeXchange with identifier PXD040240.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36903260", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Proteomics data are available via ProteomeXchange with identifier PXD040240."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Label-free proteomic analysis of lymphoblastoid cell lines from sporadic ALS and healthy donors identified 120 differentially present proteins, including 52 downregulated and 68 upregulated in ALS.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36903260", "locator": "sections[2] \"2. 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