Single cell sequencing analysis constructed the N7-methylguanosine (m7G)-related prognostic signature in uveal melanoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36920166", "kind": "science-record", "revision": 3, "content_hash": "sha256:ec49aa15f28de704ef930a10e8af8014ffdc0d2b6438136f176a0a74d2ea2218", "created_at": "2026-11-01T19:01:33Z", "updated_at": "2026-11-01T22:09:04Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Single cell sequencing analysis constructed the N7-methylguanosine (m7G)-related prognostic signature in uveal melanoma.", "doi": "10.18632/aging.204592", "pmid": "36920166", "authors": ["Jiaheng Xie", "Liang Chen", "Yuan Cao", "Chenfeng Ma", "Wenhu Zhao", "JinJing Li", "Wen Yao", "Yiming Hu", "Ming Wang", "Jingping Shi"], "journal": "Aging", "year": 2023, "abstract": "BACKGROUND: Uveal melanoma is a highly malignant tumor in the eye. Its recurrence and metastasis are common, and the prognosis is poor.\nMETHODS: The transcriptome data of UVM were downloaded from TCGA database, and the single cell sequencing dataset GSE139829 was downloaded from GEO database. Weighted co-expression network analysis was used to explore the modules associated with m7G. Lasso regression was used to construct M7G-related prognostic signature. Immune infiltration analysis was used to explore the significance of the model in the tumor immune microenvironment. Finally, cell assays were used to explore the function of key genes in the MUM-2B and OCM-1 cell lines of UVM.\nRESULTS: The prognostic signature was constructed by Cox regression and Lasso regression. Patients could be divided into high-risk group and low-risk group by this signature, and the high-risk group had worse prognosis (P<0.05). Cell experiments showed that the proliferation, invasion and migration ability of UVM cell lines were significantly decreased after the knockdown of PAG1, a key gene in signature, which proved that PAG1 might be a potential target of UVM.\nCONCLUSIONS: Our study explored the significance of m7G in UVM, provided biomarkers for its diagnosis and treatment.", "claims": [{"id": "claim-method", "statement": "The least absolute shrinkage and selection operator (LASSO) regression was performed to construct a prognostic signature.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36920166", "locator": "sections[5] \"Establishment of an m7G-related gene signature for prognosis\" paragraph 2", "quote": "The least absolute shrinkage and selection operator (LASSO) regression was performed to construct a prognostic signature."}], "state": "complete"}, {"id": "claim-data-access", "statement": "TCGA and GEO databases.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36920166", "locator": "sections[14] \"Availability of data and materials\" paragraph 2", "quote": "TCGA and GEO databases."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36920166"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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