Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36923944", "kind": "science-record", "revision": 4, "content_hash": "sha256:140b952cb1389e3419f26450c7db25b4dbd3acc85513b5a6a306dec77ba6a35e", "created_at": "2026-11-01T19:01:34Z", "updated_at": "2026-11-01T22:51:30Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Inactivation of Exosc10 in the oocyte impairs oocyte development and maturation, leading to a depletion of the ovarian reserve in mice.", "doi": "10.7150/ijbs.72889", "pmid": "36923944", "authors": ["Leïla Demini", "Christine Kervarrec", "Laëtitia Guillot", "Emmanuelle Com", "Régis Lavigne", "Pierre-Yves Kernanec", "Michael Primig", "Charles Pineau", "Fabrice G Petit", "Soazik P Jamin"], "journal": "International journal of biological sciences", "year": 2023, "abstract": "EXOSC10 is a catalytic subunit of the nuclear RNA exosome, and possesses a 3'-5' exoribonuclease activity. The enzyme processes and degrades different classes of RNAs. To delineate the role of EXOSC10 during oocyte growth, specific Exosc10 inactivation was performed in oocytes from the primordial follicle stage onward using the Gdf9-iCre; Exosc10 f/- mouse model (Exosc10 cKO(Gdf9)). Exosc10 cKO(Gdf9) female mice are infertile. The onset of puberty and the estrus cycle in mutants are initially normal and ovaries contain all follicle classes. By the age of eight weeks, vaginal smears reveal irregular estrus cycles and mutant ovaries are completely depleted of follicles. Mutant oocytes retrieved from the oviduct are degenerated, and occasionally show an enlarged polar body, which may reflect a defective first meiotic division. Under fertilization conditions, the mutant oocytes do not enter into an embryonic development process. Furthermore, we conducted a comparative proteome analysis of wild type and Exosc10 knockout mouse ovaries, and identified EXOSC10-dependent proteins involved in many biological processes, such as meiotic cell cycle progression and oocyte maturation. Our results unambiguously demonstrate an essential role for EXOSC10 in oogenesis and may serve as a model for primary ovarian insufficiency in humans. Data are available via ProteomeXchange with identifier PXD039417.", "claims": [{"id": "claim-method", "statement": "Follicular distribution was statistically tested using an unpaired two-way ANOVA. Other results were tested using an unpaired parametric Student's test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36923944", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "Follicular distribution was statistically tested using an unpaired two-way ANOVA. 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