Coronary artery disease-associated immune gene RBP1 and its pan-cancer analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36970364", "kind": "science-record", "revision": 2, "content_hash": "sha256:ec25ed2599e7dda230d48e97fdaa4432d6f2fd4f8d0e9b9759766b0c92a6378a", "created_at": "2026-11-01T19:01:37Z", "updated_at": "2026-11-01T21:30:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Coronary artery disease-associated immune gene RBP1 and its pan-cancer analysis.", "doi": "10.3389/fcvm.2023.1091950", "pmid": "36970364", "authors": ["Yumian Wang", "Li Zhang", "Han Chen", "Juan Yang", "Yun Cui", "Hong Wang"], "journal": "Frontiers in cardiovascular medicine", "year": 2023, "abstract": "Purpose: To identify immune-related biomarkers in coronary artery disease (CAD), investigate their possible function in the immunological milieu of tumors, and initially investigate the mechanisms and therapeutic targets shared by CAD and cancer.\nMethods: Download the CAD-related dataset GSE60681 from the GEO database. GSVA and WGCNA analyses were performed based on the GSE60681 dataset to identify the modules most pertinent to CAD, identify candidate hub genes and finally intersect the genes associated with immunity downloaded from the import database to find the hub genes. The GTEx, CCLE, and TCGA database were used to examine the expression of the hub gene in normal tissues, tumor cell lines, tumor tissues, and different tumor STAGES. One-factor cox and Kaplan-Meier analyses were performed to explore the prognosis of hub genes. Hub gene methylation levels in CAD and cancer were analyzed in the diseaseMeth 3.0 and ualcan databases, respectively. R package CiberSort processed the GSE60681 dataset to assess immune infiltration in CAD. TIMER2.0 evaluated hub genes with pan-cancer immune infiltration. The hub genes were analyzed for drug sensitivity and correlation with TMB, MSI, MMR, cancer-related functional status, and immune checkpoints in different tumors. Finally, GSEA was carried out on the crucial genes.\nResults: WGCNA were used to pinpoint the green modules that were most closely related to CAD and intersections with immune-related genes were taken to remember the pivotal gene RBP1. RBP1 is hypermethylated in CAD and multiple cancers. Its expression levels in different cancers were associated with poor prognosis of cancer, with significant expression levels at higher stages of cancer staging. The immune infiltration results showed that RBP1 was closely associated with CAD and tumor-associated immune infiltration. The results indicated that RBP1 was strongly correlated with TMB, MSI, MMR, cancer-associated functional status, and immune checkpoints in various cancers. RBP1 was related to the sensitivity of six anticancer drugs. GSEA showed RBP1 was associated with immune cell activation, immune response, and cancer development.\nConclusion: RBP1 is a pivotal gene associated with immunity in CAD and pan-cancer and may mediate the development of CAD and cancer through immunity, making it a common therapeutic target for both.", "claims": [{"id": "claim-method", "statement": "Also, for analysis, we employed Kaplan-Meier curves to understand the effect of mutations on patients' overall survival (OS). In addition, Spearman's test was used to analyze the relationship between RBP1 expression and TMB and MSI of various tumors in the TCGA database.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36970364", "locator": "sections[13] \"Evaluation of RBP1 mutation, TMB, and MSI in pan-cancer\" paragraph 2", "quote": "Also, for analysis, we employed Kaplan-Meier curves to understand the effect of mutations on patients' overall survival (OS). In addition, Spearman's test was used to analyze the relationship between RBP1 expression and TMB and MSI of various tumors in the TCGA database."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36970364", "locator": "sections[32] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "RBP1 is a pivotal gene associated with immunity in CAD and pan-cancer and may mediate the development of CAD and cancer through immunity, making it a common therapeutic target for both.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:36970364", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "RBP1 is a pivotal gene associated with immunity in CAD and pan-cancer and may mediate the development of CAD and cancer through immunity, making it a common therapeutic target for both."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "RBP1 is a pivotal gene associated with immunity in CAD and pan-cancer and may mediate the development of CAD and cancer through immunity, making it a common therapeutic target for both.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:36970364"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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