SERPINF1 Mediates Tumor Progression and Stemness in Glioma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:36980858", "kind": "science-record", "revision": 3, "content_hash": "sha256:145a1865fd321174761d3e1e50b5776cbd22ebf2f7c0fa3bb959b34e6d94de8e", "created_at": "2026-11-01T19:01:38Z", "updated_at": "2026-11-01T22:09:09Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "SERPINF1 Mediates Tumor Progression and Stemness in Glioma.", "doi": "10.3390/genes14030580", "pmid": "36980858", "authors": ["Lairong Song", "Xulei Huo", "Xiaojie Li", "Xiaoying Xu", "Yi Zheng", "Da Li", "Junting Zhang", "Ke Wang", "Liang Wang", "Zhen Wu"], "journal": "Genes", "year": 2023, "abstract": "Serpin family F member 1 (SERPINF1) reportedly plays multiple roles in various tumors; however, its clinical significance and molecular functions in glioma have been largely understudied. In the present study, we analyzed the prognostic value of SERPINF1 in three independent glioma datasets. Next, we explored the molecular functions and transcriptional regulation of SERPINF1 at the single-cell level. Moreover, in vitro experiments were conducted to evaluate the roles of SERPINF1 in the proliferation, invasion, migration, and stemness of glioma cells. Our results showed that a higher expression of SERPINF1 correlated with a poor overall survival rate in glioma patients (hazard ratio: 4.061 in TCGA, 2.017 in CGGA, and 1.675 in GSE16011, p < 0.001). Besides, SERPINF1 knockdown could suppress the proliferation, invasion, and migration of glioma cells in vitro. In addition, SERPINF1 expression was significantly upregulated in glioma stem cells (GSCs) compared to parental glioma cells. Knocking down SERPINF1 impaired the sphere formation of GSC-A172 and GSC-LN18. Bioinformatics analysis revealed that Notch signaling activation was closely associated with high SERPINF1 expression at the single-cell level. Furthermore, STAT1, CREM, and NR2F2 may participate in the transcriptional regulation of SERPINF1 in glioma. Overall, our results suggest that SERPINF1 may be a candidate prognostic predictor and potential therapeutic target for glioma.", "claims": [{"id": "claim-method", "statement": "Kaplan–Meier survival analysis was used to evaluate the stratification ability of SERPINF1.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:36980858", "locator": "sections[3] \"2.1. Patients and Datasets\" paragraph 2", "quote": "Kaplan–Meier survival analysis was used to evaluate the stratification ability of SERPINF1."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data analyzed in this study can be found in online repositories. 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Besides, SERPINF1 knockdown could suppress the proliferation, invasion,", "article/10.3390/genes14030580/s1, Figure S1: Knockdown efficiency of SERPINF1 in A172 and LN18 veri", "gical images of A172 and LN18 after SERPINF1 knockdown; Figure S3: ChIP-seq data of the TFs in Cist"], "order_basis": "paper_text_order:790", "id": "assay:1"}, {"role": "data_generation", "definition_id": "rna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["rials and Methods 2.1. Patients and Datasets RNA-sequencing (RNA-seq) data and clinical information of 6", "s 2.1. 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