Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37056910", "kind": "science-record", "revision": 4, "content_hash": "sha256:0bc3bd14369ec1af7f4bf4b88ec78d67b3fee1ede072c89e41960593451e3c52", "created_at": "2026-11-01T19:01:42Z", "updated_at": "2026-11-01T22:51:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Protective Effect of Qingchang Wenzhong Decoction on Colitis and Colitis-Related Carcinogenesis by Regulating Inflammation and Intestinal Fibrosis.", "doi": "10.2147/JIR.S402395", "pmid": "37056910", "authors": ["Yuan Cheng", "Junxiang Li", "Xiaosi Zhang", "Yalan Li", "Xiaojun Shi", "Rui Shi", "Tangyou Mao", "Fushun Kou", "Lei Shi"], "journal": "Journal of inflammation research", "year": 2023, "abstract": "Purpose: Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by mucosal inflammation, which may develop into ulcerative colitis-associated carcinogenesis (UCAC) with disease progression. Qingchang Wenzhong Decoction (QCWZD) is a classic and effective prescription for the clinical treatment of UC. QCWZD has been shown to alleviate intestinal mucosal injury in acute and chronic UC models. This study aimed to explore and then verify the pharmacological mechanisms of QCWZD in UC and UCAC therapy.\nMethods: In this study, approaches including microarray analysis, network pharmacology, and biological verification are employed to clarify the mechanism of QCWZD in the treatment of UC and UCAC. TCMSP, Swiss Target Prediction, and Similarity Ensemble Approach were used to investigate the active ingredients and targets of QCWZD. UC and UCAC valid targets were identified by the microarray data in the GEO database (GSE38713 and GSE47908). The core targets were obtained by PPI network and enriched by GO and KEGG. DSS and AOM/DSS mouse models were adopted to verify the above analysis results.\nResults: The enrichment analysis showed that the therapeutic targets of QCWZD enriched in blood circulation, cell adhesion molecules, and pathways of inflammation and cancer such as IL-17 signaling pathway and toll-like receptor signaling pathway were involved in the multiple synergies of QCWZD on UC and UCAC treatment. The results of experiments demonstrated that QCWZD can exert its effects on protecting the intestinal mucosal barrier, regulating inflammation and improving intestinal fibrosis in UC and UCAC and the main mechanism of QCWZD in treatment of UC and UCAC may be related to the activation of the IL-17, NF-κB and TLR4 signaling pathways.\nConclusion: Our results indicated that QCWZD treated UC and UCAC via multiple targets and pathways and the IL-17, NF-κB and TLR4 signaling pathways may be highly involved in this process.", "claims": [{"id": "claim-method", "statement": "The statistical significance for multiple comparisons was determined by carrying out analysis of variance (ANOVA) and Dunnett’s T3 tests. Under the assumption of equal variances between groups, the least significant difference (LSD) test was performed. When the data were abnormally distributed, the median and interquartile range (IQR) were commonly used. Kruskal–Wallis H(K) test and Nemenyi test (R, PMCMRplus) were chosen to determine statistical significance for multiple comparisons.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37056910", "locator": "sections[16] \"Statistical Analysis\" paragraph 2", "quote": "The statistical significance for multiple comparisons was determined by carrying out analysis of variance (ANOVA) and Dunnett’s T3 tests. Under the assumption of equal variances between groups, the least significant difference (LSD) test was performed. When the data were abnormally distributed, the median and interquartile range (IQR) were commonly used. Kruskal–Wallis H(K) test and Nemenyi test (R, PMCMRplus) were chosen to determine statistical significance for multiple comparisons."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data used to support the findings of this study are available from the corresponding author upon request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37056910", "locator": "sections[29] \"Data Sharing Statement\" paragraph 2", "quote": "The data used to support the findings of this study are available from the corresponding author upon request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "QCWZD treated ulcerative colitis and ulcerative colitis-associated carcinogenesis through multiple targets and pathways, with the IL-17, NF-κB and TLR4 signaling pathways potentially highly involved.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37056910", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Our results indicated that QCWZD treated UC and UCAC via multiple targets and pathways and the IL-17, NF-κB and TLR4 signaling pathways may be highly involved in this process."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "QCWZD treated ulcerative colitis and ulcerative colitis-associated carcinogenesis through multiple targets and pathways, with the IL-17, NF-κB and TLR4 signaling pathways potentially highly involved.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37056910"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:accession_absent"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37056910", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.2147/JIR.S402395", "sha256": "sha256:ff846acd42800df2d10fd42cb6a2887c2a2963b9725ff4e4c2c34e24a3c38272", "bytes": 53060, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.2147/JIR.S402395", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10089279", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}], "gaps": []}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "group_comparison", "specific": "anova", "label": "Group comparison", "dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["two_or_more_groups_continuous"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The statistical significance for multiple comparisons was determined by carrying out analysis of variance (ANOVA) and Dunnett’s T3 tests. Under the assumption of equal variances between groups, the least significant difference (LSD) test was performed. When the data were abnormally distributed, the median and interquartile range (IQR) were commonly used. Kruskal–Wallis H(K) test and Nemenyi test (R, PMCMRplus) were chosen to determine statistical significance for multiple comparisons."}}, {"id": "method-2", "family": "network_analysis", "specific": "ppi_network", "label": "Network / pathway analysis", "dsl_operations": ["build_graph", "centrality_scores", "pathway_enrich"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["edge_list"], "evidence": {"score": 0.7, "band": "medium", "quote": "PPI"}}], "targets": [], "gaps": []}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "data", "blocked_on": "data:accession_absent", "axes": {"method": {"tier": "R1", "expressible": true, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "missing_operations": [], "basis": "The paper names the method it used and the corpus's method extractor maps it onto the DSL catalogue: anova."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R4", "reachable": false, "accessions": ["GSE38713 and GSE47908"], "resolved_accessions": [], "statements": ["The data used to support the findings of this study are available from the corresponding author upon request."], "basis": "The paper names GSE38713 and GSE47908, but the accession does not resolve in the repository (no reason recorded), so the data cannot be reached from here. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "assets": ["source:pmid:37056910"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:None"]}, "gaps": ["data:accession_absent"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T18:36:22Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 15280, "completion_tokens": 1091, "cached_tokens": 256}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:37056910", "type": "paper", "label": "pmid:37056910"}, {"id": "source:pmid:37056910", "type": "manuscript", "label": "PMID 37056910 full text"}, {"id": "data:geo:None", "type": "dataset", "label": "GEO None"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:37056910", "type": "paper", "label": "pmid:37056910"}, {"id": "source:pmid:37056910", "type": "manuscript", "label": "PMID 37056910 full text"}, {"id": "data:geo:None", "type": "dataset", "label": "GEO None"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-37056910", "type": "extraction", "used": ["source:pmid:37056910"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-None", "type": "accession_resolution", "used": ["data:geo:None"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:37056910", "parent_hash": "sha256:ef08353977dd7b211d5fe86dd24c609684af24e753669e68281c2a5aa4c6c318", "fork_of": null, "generation": 3}, "gaps": ["execution.not_run", "review.human_critique", "review.independent_reader", "review.machine_critique", "step:obtain_source_data blocked on data:accession_absent"], "assays": [{"role": "data_generation", "definition_id": "microarray", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Methods: In this study, approaches including microarray analysis, network pharmacology, and biologic", "nd UCAC valid targets were identified by the microarray data in the GEO database (GSE38713 and GSE47"], "order_basis": "paper_text_order:672", "id": "assay:1"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["and enriched by GO and KEGG. DSS and AOM/DSS mouse models were adopted to verify the above anal", "ihua Experimental Animal Technology Co. Ltd. Mice were housed under standard environmental conditio"], "order_basis": "paper_text_order:1159", "id": "assay:2"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["group_summary", "welch_t", "bootstrap_mean_difference", "group_comparison_anova"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The statistical significance for multiple comparisons was determined by carrying out analysis of variance (ANOVA) and Dunnett’s T3 tests. Under the assumption of equal variances between groups, the least significant difference (LSD) test was performed. When the data were abnormally distributed, the median and interquartile range (IQR) were commonly used. Kruskal–Wallis H(K) test and Nemenyi test (R, PMCMRplus) were chosen to determine statistical significance for multiple comparisons."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}