hsa-miR-508-5p as a New Potential Player in Intervertebral Disc Degeneration.

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However, the underlying mechanisms of IDD remain to be determined. This study aimed to scrutinize data sets via bioinformatics to identify microRNAs (miRNAs)/genes and pathways associated with IDD. The array profiling of patients with IDD and individuals without IDD was acquired from the Gene Expression Omnibus (GEO) database (viz., GSE19943, GSE63492, and GSE34095). The expression profiles of miRNAs and genes with differential patterns were analyzed using GEO2R. The target genes of the chosen miRNA were then examined, and in silico functional analyses were performed on the signaling pathways and biological processes of the differentially expressed genes. Three human miRNAs were up and downregulated in IDD patients in the examined data sets. Among them, hsa-miR-508-5p had a significant differential expression in the IDD group, and SEC11A, IPO5, FN1, and MRPS10, as the targets of hsa-miR-508-5p, were upregulated in the IDD group. Furthermore, extracellular matrix-receptor interactions, focal adhesion, and actin cytoskeleton regulation were important pathways involved in IDD. Our analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies. Our findings not only further confirmed the significant role of miRNAs in IDD pathogenesis but also extended the spectrum of the miRNAs and genes involved in IDD. Though, still, further experimental investigations are needed to confirm our findings.", "claims": [{"id": "claim-method", "statement": "The profiles of miRNAs and mRNAs differentially expressed between the normal and degenerated samples were investigated by GEO2R for all the data sets.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37091041", "locator": "sections[2] \"Materials and Methods\" paragraph 3", "quote": "The profiles of miRNAs and mRNAs differentially expressed between the normal and degenerated samples were investigated by GEO2R for all the data sets."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data source was a publicly accessible database, and no human contributors were involved directly in the present study.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37091041", "locator": "sections[7] \"Declaration\" paragraph 2", "quote": "The data source was a publicly accessible database, and no human contributors were involved directly in the present study."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37091041", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Our analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies."}, {"asset": "source:pmid:37091041", "locator": "sections[3] \"Results\" paragraph 8", "quote": "hsa-miR-508-5p, which showed the highest differential expression, was chosen for further analysis owing to its significant logFC and P-value."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The analysis identified hsa-miR-508-5p as a novel miRNA involved in IDD pathogenies.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37091041"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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