High expression of PPFIA1 in human esophageal squamous cell carcinoma correlates with tumor metastasis and poor prognosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37158817", "kind": "science-record", "revision": 3, "content_hash": "sha256:d1369e14b2daa868765bc203edb9d4bd60129af3afd82dacf1f71e2929d9d0a2", "created_at": "2026-11-01T19:01:49Z", "updated_at": "2026-11-01T22:09:25Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "High expression of PPFIA1 in human esophageal squamous cell carcinoma correlates with tumor metastasis and poor prognosis.", "doi": "10.1186/s12885-023-10872-9", "pmid": "37158817", "authors": ["Yongyin Gao", "Lizhao Guan", "Ran Jia", "Wanyi Xiao", "Youming Han", "Yue Li", "Peng Tang", "Zhentao Yu", "Hongdian Zhang"], "journal": "BMC cancer", "year": 2023, "abstract": "BACKGROUND: PTPRF interacting protein alpha 1 (PPFIA1) is reportedly related to the occurrence and progression of several kinds of malignancies. However, its role in esophageal squamous cell carcinoma (ESCC) is unclear. This current study investigated the prognostic significance and biological functions of PPFIA1 in ESCC.\nMETHODS: Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), and Gene Expression Omnibus (GEO) were used to investigate PPFIA1 expression in esophageal cancer. The relationship between PPFIA1 expression and clinicopathological characteristics and patient survival was evaluated in GSE53625 dataset, and verified in the cDNA array based on qRT-PCR and tissue microarray (TMA) dataset based on immunohistochemistry. The impact of PPFIA1 on the migration and invasion of cancer cells were investigated by wound-healing and transwell assays, respectively.\nRESULTS: The expression of PPFIA1 was obviously increased in ESCC tissues versus adjacent esophageal tissues according to online database analyses (all P < 0.05). High PPFIA1 expression was closely related to several clinicopathological characteristics, including tumor location, histological grade, tumor invasion depth, lymph node metastasis, and tumor-node-metastasis (TNM) stage. High PPFIA1 expression was related to worse outcomes and was identified as an independent prognostic factor of overall survival in ESCC patients (GSE53625 dataset, P = 0.019; cDNA array dataset, P < 0.001; TMA dataset, P = 0.039). Downregulation of PPFIA1 expression can significantly reduce the migration and invasion ability of ESCC cells.\nCONCLUSION: PPFIA1 is related to the migration and invasion of ESCC cells, and can be used as a potential biomarker to evaluate the prognosis of ESCC patients.", "claims": [{"id": "claim-method", "statement": "Survival analysis was carried out through the Kaplan–Meier method and the log-rank test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37158817", "locator": "sections[14] \"Statistical analyses\" paragraph 2", "quote": "Survival analysis was carried out through the Kaplan–Meier method and the log-rank test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets that support the findings of this study are available from third parties, with the weblinks provided in the manuscript methodology section and can be located at the following sites: GEPIA (http://gepia.cancer-pku.cn/detail.php), Oncomine (http://www.oncomine.org), GEO database (https://www.ncbi.nlm.nih.gov/geo/), GSE53625 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625). TCGA database (https://cancergenome.nih.gov/), Kaplan–Meier Plotter (www.kmplot.com) and STRING (http://string-db.org/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37158817", "locator": "sections[32] \"notes\" paragraph 1", "quote": "The datasets that support the findings of this study are available from third parties, with the weblinks provided in the manuscript methodology section and can be located at the following sites: GEPIA (http://gepia.cancer-pku.cn/detail.php), Oncomine (http://www.oncomine.org), GEO database (https://www.ncbi.nlm.nih.gov/geo/), GSE53625 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625). TCGA database (https://cancergenome.nih.gov/), Kaplan–Meier Plotter (www.kmplot.com) and STRING (http://string-db.org/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High PPFIA1 expression was related to worse outcomes and was identified as an independent prognostic factor of overall survival in ESCC patients (GSE53625 dataset, P = 0.019; cDNA array dataset, P < 0.001; TMA dataset, P = 0.039).", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37158817", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "High PPFIA1 expression was related to worse outcomes and was identified as an independent prognostic factor of overall survival in ESCC patients (GSE53625 dataset, P = 0.019; cDNA array dataset, P < 0.001; TMA dataset, P = 0.039)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High PPFIA1 expression was related to worse outcomes and was identified as an independent prognostic factor of overall survival in ESCC patients (GSE53625 dataset, P = 0.019; cDNA array dataset, P < 0.001; TMA dataset, P = 0.039).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37158817"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE20347"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE20347"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37158817", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s12885-023-10872-9", "sha256": "sha256:80b114f59feceda642fb257a8b65337b6ebf5e476918ba32f1b8bb237801dbd6", "bytes": 35693, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s12885-023-10872-9", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10169376", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE20347", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE20347", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE20347", "version": null, "note": "The datasets that support the findings of this study are available from third parties, with the weblinks provided in the manuscript methodology section and can be located at the following sites: GEPIA (http://gepia.cancer-pku.cn/detail.php), Oncomine (http://www.oncomine.org), GEO database (https://www.ncbi.nlm.nih.gov/geo/), GSE53625 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625). 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TCGA database (https://cancergenome.nih.gov/), Kaplan–Meier Plotter (www.kmplot.com) and STRING (http://string-db.org/).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM509787", "GSM509788", "GSM509789", "GSM509790", "GSM509791", "GSM509792", "GSM509793", "GSM509794", "GSM509795", "GSM509796", "GSM509797", "GSM509798", "GSM509799", "GSM509800", "GSM509801", "GSM509802", "GSM509803", "GSM509804", "GSM509805", "GSM509806", "GSM509807", "GSM509808", "GSM509809", "GSM509810", "GSM509811", "GSM509812", "GSM509813", "GSM509814", "GSM509815", "GSM509816", "GSM509817", "GSM509818", "GSM509819", "GSM509820"], "sample_count": 34, "group_sizes": null, "group_sizes_basis": null, "platform": "571", "taxon": "Homo sapiens", "title": "Analysis of gene expression in esophageal squamous cell carcinoma (ESCC)", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE23400", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE23400", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE23400", "version": null, "note": "The datasets that support the findings of this study are available from third parties, with the weblinks provided in the manuscript methodology section and can be located at the following sites: GEPIA (http://gepia.cancer-pku.cn/detail.php), Oncomine (http://www.oncomine.org), GEO database (https://www.ncbi.nlm.nih.gov/geo/), GSE53625 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE53625). 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A Negative PPFIA1 expression in sections of", ", TNM tumor-node-metastasis, TMA TMA, tissue microarray Correlations between PPFIA1 expression and t"], "order_basis": "paper_text_order:774", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["and tissue microarray (TMA) dataset based on immunohistochemistry. The impact of PPFIA1 on the migration and i", "assessed PPFIA1 protein expression using an IHC staining-based TMA dataset containing sample"], "order_basis": "paper_text_order:808", "id": "assay:2"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ified by the 2−∆∆CT method. Cell culture and transfection The human ESCC cell lines Kyse-30 and Ec-109", "hanghai, China). ESCC cells were transiently transfected with these siRNAs using Lipofectamine 2000 r", "the manufacturer's instructions. 48 h after transfection, the RNA and protein were harvested and anal", "ing 10% fetal bovine serum (Gibco, USA). 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