The combination therapy of isomucronulatol 7-O-beta-glucoside (IMG) and CEP-9722 targeting ferroptosis-related biomarkers in non-small cell lung cancer (NSCLC).

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37165402", "kind": "science-record", "revision": 4, "content_hash": "sha256:8fd3e664eb669f1774e36ec9a9afa83cd2771beee0931f8dcb090bf22a9c706b", "created_at": "2026-11-01T19:01:50Z", "updated_at": "2026-11-01T22:51:51Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The combination therapy of isomucronulatol 7-O-beta-glucoside (IMG) and CEP-9722 targeting ferroptosis-related biomarkers in non-small cell lung cancer (NSCLC).", "doi": "10.1186/s12890-023-02445-0", "pmid": "37165402", "authors": ["Xiaofei Cui", "Chang Liu", "Penghua Dong", "Chao Liu", "Yu Bai"], "journal": "BMC pulmonary medicine", "year": 2023, "abstract": "BACKGROUND: NSCLC is a malignant tumor with a high incidence. Ferroptosis presents an essential function in regulating carcinogenesis and tumor progression. However, the ferroptosis-associated prognostic model based on single-cell sequencing of NSCLC remains unexplored. Our study aims to establish a potential predictive model for NSCLC patients and provide available targeted drugs for clinical treatment.\nMETHODS: The data on NSCLC patients were collected from TCGA and GEO databases to analyze their gene expression profiles. ConsensusCluster was adopted to divide the patients into different groups based on ferroptosis-related genes. Then, the univariable Cox and LASSO analyses were applied to data analysis and model establishment. Single-cell analysis was used to explore the risk score genes in different cell populations and states. The protein levels of these genes were also investigated through the HPA database. Drug sensitivity was evaluated in CellMiner database. CCK8 and colony formation assays were performed to validate potential drugs' effects on lung cancer cell lines.\nRESULTS: A ferroptosis-related prognostic model involving 14 genes in NSCLC patients was established. The risk score model was developed in training set GSE31210 and validated in the test set TCGA. The low-risk score group showed a better prognosis than the high-risk score group. The single-cell analysis revealed that the risk score genes were mainly derived from lung tumor cells. Most risk score genes were more highly expressed in tumor tissue than in normal tissue, according to the HPA database. Besides, these genes were associated with 106 drugs in CellMiner database. Finally, the drug effects on NSCLC cell growth were evaluated by cck8 and colony formation.\nCONCLUSIONS: We identified an effective ferroptosis-related prognostic model based on single-cell sequencing. The potential prediction model is devoted to exploring clinical therapeutic targets for NSCLC.", "claims": [{"id": "claim-method", "statement": "The relationship between genes and overall survival (OS) was analyzed by using LASSO COX regression analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37165402", "locator": "sections[6] \"Development and validation of prognostic risk models\" paragraph 2", "quote": "The relationship between genes and overall survival (OS) was analyzed by using LASSO COX regression analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used in this study are available from the TCGA database (https://xenabrowser.net/datapages/), GEO database (https://www.ncbi.nlm.nih.gov/), the HPA database (https://www.proteinatlas.org/), the CellMiner database (https://discover.nci.nih.gov/cellminer/home.do), the TCMSP databse (https://old.tcmsp-e.com/tcmsp.php), and the RCSB PDB database (https://www.rcsb.org/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37165402", "locator": "sections[33] \"notes\" paragraph 1", "quote": "The datasets used in this study are available from the TCGA database (https://xenabrowser.net/datapages/), GEO database (https://www.ncbi.nlm.nih.gov/), the HPA database (https://www.proteinatlas.org/), the CellMiner database (https://discover.nci.nih.gov/cellminer/home.do), the TCMSP databse (https://old.tcmsp-e.com/tcmsp.php), and the RCSB PDB database (https://www.rcsb.org/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A ferroptosis-related prognostic model involving 14 genes in NSCLC patients was established, with the low-risk score group showing a better prognosis than the high-risk score group.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37165402", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "A ferroptosis-related prognostic model involving 14 genes in NSCLC patients was established."}, {"asset": "source:pmid:37165402", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "The low-risk score group showed a better prognosis than the high-risk score group."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A ferroptosis-related prognostic model involving 14 genes in NSCLC patients was established, with the low-risk score group showing a better prognosis than the high-risk score group.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37165402"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE31210", "GSE30219", "GSE131907"], "resolved_accessions": ["GSE31210", "GSE30219", "GSE131907"], "statements": ["The datasets used in this study are available from the TCGA database (https://xenabrowser.net/datapages/), GEO database (https://www.ncbi.nlm.nih.gov/), the HPA database (https://www.proteinatlas.org/), the CellMiner database (https://discover.nci.nih.gov/cellminer/home.do), the TCMSP databse (https://old.tcmsp-e.com/tcmsp.php), and the RCSB PDB database (https://www.rcsb.org/)."], "basis": "The paper names GSE31210, GSE30219, GSE131907 in GEO and the accession resolves in the repository. 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CCK8 and colony formation assays were performed t", "fects on NSCLC cell growth were evaluated by cck8 and colony formation. CONCLUSIONS: We identi"], "order_basis": "paper_text_order:1097", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["lture collection (ATCC). The A549 cells were cultured in McCoy’s 5A medium, supplemented with 10% bov", "lines and cell culture Lung cancer cell line A549 was provided by American type culture collec", "American type culture collection (ATCC). The A549 cells were cultured in McCoy’s 5A medium, su"], "order_basis": "paper_text_order:2142", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["The relationship between genes and overall survival (OS) was analyzed by using LASSO COX regression analysis."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}