Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37189386", "kind": "science-record", "revision": 2, "content_hash": "sha256:c6c3455d460ac7082c63fd93e2320bd87974096b86730896d00ad93857f16bd3", "created_at": "2026-11-01T19:01:52Z", "updated_at": "2026-11-01T21:30:46Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Integrative Analysis and Experimental Validation of Competing Endogenous RNAs in Obstructive Sleep Apnea.", "doi": "10.3390/biom13040639", "pmid": "37189386", "authors": ["Niannian Li", "Yaxin Zhu", "Feng Liu", "Xiaoman Zhang", "Yuenan Liu", "Xiaoting Wang", "Zhenfei Gao", "Jian Guan", "Shankai Yin"], "journal": "Biomolecules", "year": 2023, "abstract": "BACKGROUND: Obstructive sleep apnea (OSA) is highly prevalent yet underdiagnosed. This study aimed to develop a predictive signature, as well as investigate competing endogenous RNAs (ceRNAs) and their potential functions in OSA.\nMETHODS: The GSE135917, GSE38792, and GSE75097 datasets were collected from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database. Weighted gene correlation network analysis (WGCNA) and differential expression analysis were used to identify OSA-specific mRNAs. Machine learning methods were applied to establish a prediction signature for OSA. Furthermore, several online tools were used to establish the lncRNA-mediated ceRNAs in OSA. The hub ceRNAs were screened using the cytoHubba and validated by real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Correlations between ceRNAs and the immune microenvironment of OSA were also investigated.\nRESULTS: Two gene co-expression modules closely related to OSA and 30 OSA-specific mRNAs were obtained. They were significantly enriched in the antigen presentation and lipoprotein metabolic process categories. A signature that consisted of five mRNAs was established, which showed a good diagnostic performance in both independent datasets. A total of twelve lncRNA-mediated ceRNA regulatory pathways in OSA were proposed and validated, including three mRNAs, five miRNAs, and three lncRNAs. Of note, we found that upregulation of lncRNAs in ceRNAs could lead to activation of the nuclear factor kappa B (NF-κB) pathway. In addition, mRNAs in the ceRNAs were closely correlated to the increased infiltration level of effector memory of CD4 T cells and CD56bright natural killer cells in OSA.\nCONCLUSIONS: In conclusion, our research opens new possibilities for diagnosis of OSA. The newly discovered lncRNA-mediated ceRNA networks and their links to inflammation and immunity may provide potential research spots for future studies.", "claims": [{"id": "claim-method", "statement": "Weighted gene correlation network analysis (WGCNA) and differential expression analysis were used to identify OSA-specific mRNAs.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37189386", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Weighted gene correlation network analysis (WGCNA) and differential expression analysis were used to identify OSA-specific mRNAs."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study are openly available in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE135917/GSE38792/GSE75097/GSE99239 (accessed on 24 December 2022).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37189386", "locator": "sections[31] \"Data Availability Statement\" paragraph 2", "quote": "The datasets presented in this study are openly available in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE135917/GSE38792/GSE75097/GSE99239 (accessed on 24 December 2022)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Two gene co-expression modules closely related to OSA and 30 OSA-specific mRNAs were obtained.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37189386", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Two gene co-expression modules closely related to OSA and 30 OSA-specific mRNAs were obtained."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Two gene co-expression modules closely related to OSA and 30 OSA-specific mRNAs were obtained.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37189386"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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