Screening and identification of key chromatin regulator biomarkers for ankylosing spondylitis and drug prediction: evidence from bioinformatics analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37193965", "kind": "science-record", "revision": 4, "content_hash": "sha256:5f56db06e33caa5c77d77d534ad73aa4f022f1e8661ba96efbaa4d58ff35d7bc", "created_at": "2026-11-01T19:01:53Z", "updated_at": "2026-11-01T22:51:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Screening and identification of key chromatin regulator biomarkers for ankylosing spondylitis and drug prediction: evidence from bioinformatics analysis.", "doi": "10.1186/s12891-023-06490-y", "pmid": "37193965", "authors": ["Han Wang", "Hongbo Jin", "Zhiyang Liu", "Chengju Tan", "Lin Wei", "Mingfen Fu", "Yizhuan Huang"], "journal": "BMC musculoskeletal disorders", "year": 2023, "abstract": "BACKGROUND: Ankylosing spondylitis (AS) is one of the most common immune-mediated arthritic diseases worldwide. Despite considerable efforts to elucidate its pathogenesis, the molecular mechanisms underlying AS are still not fully understood.\nMETHODS: To identify candidate genes involved in AS progression, the researchers downloaded the microarray dataset GSE25101 from the Gene Expression Omnibus (GEO) database. They identified differentially expressed genes (DEGs) and functionally enriched them for analysis. They also constructed a protein-protein interaction network (PPI) using STRING and performed cytoHubba modular analysis, immune cell and immune function analysis, functional analysis and drug prediction.The results showed that DEGs were mainly associated with histone modifications, chromatin organisation, transcriptional coregulator activity, transcriptional co-activator activity, histone acetyltransferase complexes and protein acetyltransferase complexes.\nRESULTS: The researchers analysed the differences in expression between the CONTROL and TREAT groups in terms of immunity to determine their effect on TNF-α secretion. By obtaining hub genes, they predicted two therapeutic agents, AY 11-7082 and myricetin.\nCONCLUSION: The DEGs, hub genes and predicted drugs identified in this study contribute to our understanding of the molecular mechanisms underlying the onset and progression of AS. They also provide candidate targets for the diagnosis and treatment of AS.", "claims": [{"id": "claim-method", "statement": "To analyze the differentially expressed genes of chromatin regulatory factors between the AS and control groups, we utilized the ‘limma’ package to conduct a comprehensive analysis of the two cohorts.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37193965", "locator": "sections[4] \"Differential expression analysis and construction of heat and volcano plots\" paragraph 2", "quote": "To analyze the differentially expressed genes of chromatin regulatory factors between the AS and control groups, we utilized the ‘limma’ package to conduct a comprehensive analysis of the two cohorts."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated and/or analysed during the current study are available in the [Gene Expression Omnibus (GEO)] repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE25101].", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37193965", "locator": "sections[25] \"notes\" paragraph 1", "quote": "The datasets generated and/or analysed during the current study are available in the [Gene Expression Omnibus (GEO)] repository, [https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE25101]."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The results showed that DEGs were mainly associated with histone modifications, chromatin organisation, transcriptional coregulator activity, transcriptional co-activator activity, histone acetyltransferase complexes and protein acetyltransferase complexes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37193965", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "The results showed that DEGs were mainly associated with histone modifications, chromatin organisation, transcriptional coregulator activity, transcriptional co-activator activity, histone acetyltransferase complexes and protein acetyltransferase complexes."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The results showed that DEGs were mainly associated with histone modifications, chromatin organisation, transcriptional coregulator activity, transcriptional co-activator activity, histone acetyltransferase complexes and protein acetyltransferase complexes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37193965"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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