MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37304127", "kind": "science-record", "revision": 2, "content_hash": "sha256:de881004a01c4b84156f71f66d37243fa7a0686d8ad2d15b9f19ff9085323c4f", "created_at": "2026-11-01T19:02:02Z", "updated_at": "2026-11-01T21:30:58Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines.", "doi": "10.1155/2023/3610466", "pmid": "37304127", "authors": ["Xueheng Zhao", "Zenghui Huang", "Yongzhe Chen", "Qianyin Zhou", "Fang Zhu", "Huan Zhang", "Dai Zhou"], "journal": "Stem cells international", "year": 2023, "abstract": "Spermatogonial stem cells are committed to initiating and maintaining male spermatogenesis, which is the foundation of male fertility. Understanding the mechanisms underlying SSC fate decisions is critical for controlling spermatogenesis and male fertility. However, the key molecules and mechanisms responsible for regulating human SSC development are not clearly understood. Here, we analyzed normal human testis single-cell sequencing data from the GEO dataset (GSE149512 and GSE112013). Melanoma antigen gene B2 (MAGEB2) was found to be predominantly expressed in human SSCs and further validated by immunohistology. Overexpression of MAGEB2 in SSC lines severely weakened cell proliferation and promoted apoptosis. Further, using protein interaction prediction, molecular docking, and immunoprecipitation, we found that MAGEB2 interacted with early growth response protein 1 (EGR1) in SSC lines. Reexpression of EGR1 in MAGEB2 overexpression cells partially rescued decreased cell proliferation. Furthermore, MAGEB2 was shown to be downregulated in specific NOA patients, implying that abnormal expression of MAGEB2 may impair spermatogenesis and male fertility. Our results offer new insights into the functional and regulatory mechanisms in MAGEB2-mediated human SSC line proliferation and apoptosis.", "claims": [{"id": "claim-method", "statement": "The t-test was chosen after D'Agostino-Pearson omnibus normality tests for each data.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37304127", "locator": "sections[14] \"2.12. Statistical Analysis\" paragraph 2", "quote": "The t-test was chosen after D'Agostino-Pearson omnibus normality tests for each data."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data generated by this study can be obtained from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37304127", "locator": "sections[26] \"Data Availability\" paragraph 2", "quote": "The data generated by this study can be obtained from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "MAGEB2 was predominantly expressed in human SSCs, and its upregulation impaired proliferation and promoted apoptosis in SSC lines through an interaction with EGR1.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37304127", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Melanoma antigen gene B2 (MAGEB2) was found to be predominantly expressed in human SSCs and further validated by immunohistology."}, {"asset": "source:pmid:37304127", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Overexpression of MAGEB2 in SSC lines severely weakened cell proliferation and promoted apoptosis."}, {"asset": "source:pmid:37304127", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Further, using protein interaction prediction, molecular docking, and immunoprecipitation, we found that MAGEB2 interacted with early growth response protein 1 (EGR1) in SSC lines."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "MAGEB2 was predominantly expressed in human SSCs, and its upregulation impaired proliferation and promoted apoptosis in SSC lines through an interaction with EGR1.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37304127"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE112013"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE112013"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37304127", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2023/3610466", "sha256": "sha256:5ea5f9c7804f189591aa2b7becfbefeb460fda0575fda477646e9971f5b0f2aa", "bytes": 57321, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1155/2023/3610466", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10256451", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE112013", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE112013", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE112013", "version": null, "note": "The data generated by this study can be obtained from the corresponding author upon reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The data generated by this study can be obtained from the corresponding author upon reasonable request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM3052917", "GSM3052918", "GSM3052919", "GSM3052920", "GSM3052921", "GSM3052922"], "sample_count": 6, "group_sizes": null, "group_sizes_basis": null, "platform": "16791", "taxon": "Homo sapiens", "title": "The Human Testis Cell Atlas via Single-cell RNA-seq (Healthy men scRNA-seq data set)", "resolution_checked_at": "2026-11-01T18:46:34Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE149512", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE149512", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE149512", "version": null, "note": "The data generated by this study can be obtained from the corresponding author upon reasonable request.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "The data generated by this study can be obtained from the corresponding author upon reasonable request.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM4504181", "GSM4504182", "GSM4504183", "GSM4504184", "GSM4504185", "GSM4504186", "GSM4504187", "GSM4504188", "GSM4504189", "GSM4504190", "GSM4504191", "GSM4504192", "GSM4504193", "GSM4504194", "GSM4504195", "GSM4504196", "GSM4504197"], "sample_count": 17, "group_sizes": null, "group_sizes_basis": null, "platform": "24676;27644", "taxon": "Homo sapiens", "title": "Single-cell analysis of developing and azoospermia human testicles reveals central role of Sertoli cells", "resolution_checked_at": "2026-11-01T18:06:43Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE112013 not downloaded", "asset:data:geo:GSE112013 licence not read", "asset:data:geo:GSE149512 not downloaded", "asset:data:geo:GSE149512 licence not read"]}, "analysis": {"state": "partial", "methods": [{"id": "method-1", "family": null, "specific": null, "label": "t-test selected after D'Agostino-Pearson omnibus normality tests", "dsl_operations": [], "missing_operations": [], "expressible": null, "parameters": {}, "data_shapes": [], "evidence": {"score": 1.0, "band": "verbatim", "quote": "The t-test was chosen after D'Agostino-Pearson omnibus normality tests for each data."}}], "targets": [], "gaps": ["method.reader_named_unmapped"]}, "capability": {"state": "complete", "requirements": [{"capability_id": "capability:computational_analysis", "label": "Computational analysis platform", "assay_families": ["meta_analysis", "machine_learning", "survival_analysis", "statistical_regression", "pca", "molecular_docking", "network_analysis", "differential_expression", "gwas"], "availability_state": "owned", "tier_unlocked": "R1", "candidate": "Automated Assays Laravel application and simulated adapters"}], "gaps": []}, "readiness": {"state": "blocked", "tier": "R4", "blocking_axis": "method", "blocked_on": "method:not_determinable", "axes": {"method": {"tier": "R4", "expressible": false, "required_operations": [], "missing_operations": [], "basis": "The reader quoted the method the paper names (t-test selected after D'Agostino-Pearson omnibus normality tests), but the corpus's method extractor could not map it onto a DSL operation, so the method is published but not expressible."}, "capability": {"tier": "R1", "capability_id": "capability:computational_analysis", "availability_state": "owned", "basis": "The computational analysis is owned. The physical bench the paper used is not, so a physical validation cannot be run here."}, "data": {"tier": "R1", "reachable": true, "accessions": ["GSE112013", "GSE149512"], "resolved_accessions": ["GSE112013", "GSE149512"], "statements": ["The data generated by this study can be obtained from the corresponding author upon reasonable request."], "basis": "The paper names GSE112013, GSE149512 in GEO and the accession resolves in the repository. No dataset was downloaded and no licence was read."}}, "can_run": {"dsl_operations": [], "assets": ["source:pmid:37304127", "data:geo:GSE112013"], "steps": ["run_analysis"]}, "must_build": {"dsl_operations": [], "converters": [], "readers": [], "capabilities": [], "data_gaps": ["data:geo:GSE112013"]}, "gaps": ["method:not_determinable"]}, "review": {"state": "partial", "machine_critique": {"kind": "extraction_review", "reader": {"model": "MiniMax-M3.1-Flash", "provider": "minimax", "provider_family": "minimax", "read_at": "2026-11-01T18:43:31Z", "independence": "single_reader"}, "evidence_check": {"method": "verbatim", "availability": "verbatim"}, "calls": 5, "usage": {"prompt_tokens": 17036, "completion_tokens": 1110, "cached_tokens": 256}}, "human_critique": null, "stages": [{"id": "extraction", "state": "complete", "reader": "MiniMax-M3.1-Flash", "provider": "minimax"}], "gaps": ["review.independent_reader", "review.machine_critique", "review.human_critique"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "pmid:37304127", "type": "paper", "label": "pmid:37304127"}, {"id": "source:pmid:37304127", "type": "manuscript", "label": "PMID 37304127 full text"}, {"id": "data:geo:GSE112013", "type": "dataset", "label": "GEO GSE112013"}], "activities": [{"id": "activity-reading-sci-2026-11-01", "type": "classification", "used": [{"id": "pmid:37304127", "type": "paper", "label": "pmid:37304127"}, {"id": "source:pmid:37304127", "type": "manuscript", "label": "PMID 37304127 full text"}, {"id": "data:geo:GSE112013", "type": "dataset", "label": "GEO GSE112013"}], "agent": "agent:corpus-assay-engine"}, {"id": "activity-read-37304127", "type": "extraction", "used": ["source:pmid:37304127"], "agent": "agent:minimax-reader"}, {"id": "activity-resolve-GSE112013", "type": "accession_resolution", "used": ["data:geo:GSE112013"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}, {"id": "agent:minimax-reader", "type": "software", "label": "MiniMax literature reader"}], "source_run_id": "reading-sci-2026-11-01", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "pmid:37304127", "parent_hash": "sha256:f9b0643754183398be26cecc92b7eb79db90da12d69b625c6369b9ff359fce35", "fork_of": null, "generation": 1}, "gaps": ["asset:data:geo:GSE112013 licence not read", "asset:data:geo:GSE112013 not downloaded", "asset:data:geo:GSE149512 licence not read", "asset:data:geo:GSE149512 not downloaded", "execution.not_run", "method.reader_named_unmapped", "review.human_critique", "review.independent_reader", "review.machine_critique"], "assays": [{"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["tion of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines.", "ely weakened cell proliferation and promoted apoptosis. Further, using protein interaction predicti", "B2-mediated human SSC line proliferation and apoptosis."], "order_basis": "paper_text_order:23", "id": "assay:1"}, {"role": "data_generation", "definition_id": "scrna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "unsupported", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["als and Methods 2.1. Sources and Analysis of Single-Cell RNA Sequencing Data The normal testicular cell scRNA-seq da", "A Sequencing Data The normal testicular cell scRNA-seq datasets GSE112013 (three samples) [40] and", "://www.ncbi.nlm.nih.gov/gds). To analyze the scRNA-seq data, Seurat 4.3.0 (http://satijalab.org/seu"], "order_basis": "paper_text_order:1436", "id": "assay:2"}, {"role": "data_generation", "definition_id": "rna_seq", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ods 2.1. Sources and Analysis of Single-Cell RNA Sequencing Data The normal testicular cell scRNA-seq da"], "order_basis": "paper_text_order:1448", "id": "assay:3"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["enesis as normal controls. Then, a two-color immunofluorescence was used to analyze the distribution of MAGE", "(a) and 7(b)). Consistent with the result of immunofluorescence, the results of western blot showed that the", "nt testicular tissues detected by dual-color immunofluorescence: the green color is MAGEB2 signal, the red c", "able 1: antibodies applied in western blots, immunofluorescence, and immunoprecipitation.Click here for addi"], "order_basis": "paper_text_order:3718", "id": "assay:4"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["result of immunofluorescence, the results of western blot showed that the total level of MAGEB2 was si", "of the seminiferous tubule were counted. (c) Western blot detected the expression of MAGEB2 in differe", "Supplementary Table 1: antibodies applied in western blots, immunofluorescence, and immunoprecipitation"], "order_basis": "paper_text_order:4085", "id": "assay:5"}]}