APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37305534", "kind": "science-record", "revision": 3, "content_hash": "sha256:41b938cb14d92b95b40c10a126deb1c26e409b09816972a08ad1734d9eb02cc9", "created_at": "2026-11-01T19:02:02Z", "updated_at": "2026-11-01T22:09:40Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "APOC1 exacerbates renal fibrosis through the activation of the NF-κB signaling pathway in IgAN.", "doi": "10.3389/fphar.2023.1181435", "pmid": "37305534", "authors": ["Kuipeng Yu", "Lin Ding", "Xin An", "Yanjiang Yang", "Xiaoning Zhang", "Luyao Li", "Chunjie Wang", "Fang Bai", "Xiangdong Yang"], "journal": "Frontiers in pharmacology", "year": 2023, "abstract": "Introduction: IgA nephropathy (IgAN) is the most common disease leading to end-stage renal disease, and tubular fibrosis represents an important risk factor for disease progression. However, research on early molecular diagnostic indicators of tubular fibrosis and the mechanisms underlying disease progression is still lacking. Methods: The GSE93798 dataset was downloaded from the GEO database. DEGs were screened and analyzed for GO and KEGG enrichment in IgAN. The least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE) algorithms were applied to screen for hub secretory genes. The expression and diagnostic efficacy of hub genes were confirmed by the GSE35487 dataset. ELISA was applied to detect the expression of APOC1 in serum. The expression and localization of hub genes in IgAN were verified by the expression of IHC and IF in human kidney tissues, and the correlation of expression with clinical data was verified in the Nephroseq database. Finally, cellular experiments clarified the role of hub genes in the signaling pathway. Results: A total of 339 DEGs were identified in IgAN, of which 237 were upregulated and 102 downregulated. The KEGG signaling pathway is enriched in the ECM-receptor interaction and AGE-RAGE signaling pathway. APOC1, ALB, CCL8, CXCL2, SRPX2, and TGFBI identified six hub secretory genes using the LASSO and SVM-RFE algorithms. In vivo and in vitro experiments demonstrated that APOC1 expression was elevated in IgAN. The serum concentration of APOC1 was 1.232 ± 0.1812 μg/ml in IgAN patients, whereas it was 0.3956 ± 0.1233 μg/ml in healthy individuals. APOC1 exhibited high diagnostic efficacy for IgAN (AUC of 99.091%, specificity of 95.455%, and sensitivity of 99.141%) in the GSE93798 dataset. APOC1 expression negatively correlated with eGFR (R 2 = 0.2285, p = 0.0385) and positively correlated with serum creatinine (R 2 = 0.41, p = 0.000567) in IgAN. APOC1 exacerbated renal fibrosis, possibly in part by activating the NF-κB pathway in IgAN. Conclusion: APOC1 was identified as the core secretory gene of IgAN, which was closely associated with blood creatinine and eGFR and had significant efficacy in the diagnosis of IgAN. Mechanistic studies revealed that the knockdown of APOC1 could improve IgAN renal fibrosis by inhibiting the NF pathway, which may be a potential therapeutic target for improving renal fibrosis in IgAN.", "claims": [{"id": "claim-method", "statement": "To assess the diagnostic accuracy of APOC1 in predicting IgAN, receiver operating characteristics were evaluated.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37305534", "locator": "sections[20] \"3.4 APOC1 expression and diagnostic efficacy\" paragraph 2", "quote": "To assess the diagnostic accuracy of APOC1 in predicting IgAN, receiver operating characteristics were evaluated."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37305534", "locator": "sections[27] \"Data availability statement\" paragraph 2", "quote": "The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/Supplementary Material."}], "state": "complete"}, {"id": "claim-target-result", "statement": "APOC1 exhibited high diagnostic efficacy for IgAN in the GSE93798 dataset.", "kind": "quantitative", "target": {"value": 99.091, "unit": "auc", "tolerance": null, "source": "APOC1 exhibited high diagnostic efficacy for IgAN (AUC of 99.091%, specificity of 95.455%, and sensitivity of 99.141%) in the GSE93798 dataset."}, "evidence": [{"asset": "source:pmid:37305534", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "APOC1 exhibited high diagnostic efficacy for IgAN (AUC of 99.091%, specificity of 95.455%, and sensitivity of 99.141%) in the GSE93798 dataset."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "APOC1 exhibited high diagnostic efficacy for IgAN in the GSE93798 dataset.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37305534"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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ELISA was applied to detect the expression of APOC"], "order_basis": "paper_text_order:794", "id": "assay:1"}, {"role": "data_generation", "definition_id": "ihc", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["s in IgAN were verified by the expression of IHC and IF in human kidney tissues, and the corr", "ular epithelial cells of IgAN patients. IHC, immunohistochemical; IF, immunofluorescence; AQP1, aquaporin 1 (", "IgAN patients. IHC, immunohistochemical; IF, immunofluorescence; AQP1, aquaporin 1 (proximal renal tubular m", "g/ml in healthy individuals (Figure 6A). The IHC research revealed elevated expression of APO"], "order_basis": "paper_text_order:944", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["enes using the LASSO and SVM-RFE algorithms. In vivo and in vitro experiments demonstrated that A"], "order_basis": "paper_text_order:1488", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["IgAN. Mechanistic studies revealed that the knockdown of APOC1 could improve IgAN renal fibrosis b", "venny/index.html). 2.9 Cell culture and cell transfection HK-2 cells were cultivated in RPMI-1640 (Gib", "by Boshang Biotechnology Company, which was transfected to overexpress APOC1. jetPRIME transfection", "s transfected to overexpress APOC1. jetPRIME transfection reagent (Polyplus, France) was applied to co"], "order_basis": "paper_text_order:2334", "id": "assay:4"}, {"role": "analysis", "definition_id": "machine_learning", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["train_classifier", "cross_validate", "roc_auc"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 0.7, "evidence": ["support vector machine"], "order_basis": "consumes_earlier_output", "id": "assay:5"}]}