Construction of ceRNA regulatory networks for osteoporosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37326104", "kind": "science-record", "revision": 2, "content_hash": "sha256:68dcd8fdbbf1606ddcb4510b76a262d11ababb9a8b06fe6833acd4effbfb08ba", "created_at": "2026-11-01T19:02:03Z", "updated_at": "2026-11-01T21:31:00Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Construction of ceRNA regulatory networks for osteoporosis.", "doi": "10.3892/mmr.2023.13033", "pmid": "37326104", "authors": ["Hongtao Chen", "Hailong Wang", "Xu Liu", "Long Li", "Yiliyaer Abudusimu", "Yilihamu Tuoheti"], "journal": "Molecular medicine reports", "year": 2023, "abstract": "Osteoporosis increases the risk of fracture. Improving the diagnosis and treatment of osteoporosis has clinical applications. The differentially expressed genes (DEcircRs, DEmRs, DEmiRs) of osteoporotic patients and controls were analyzed using the GEO database, and enrichment analysis of DEmRs was performed. circRNAs and mRNAs, which were predicted to have a target relationship with DEmRs, were obtained to compare competing endogenous RNA (ceRNA) regulatory networks by comparison with differentially expressed genes. Molecular experiments were utilized to validate the expression of genes within the network. The interactions between genes within the ceRNA network were validated by luciferase reporter assays. Following overexpression of circ_0070304 in bone marrow mesenchymal stem cells (BMSCs), the osteogenic differentiation of the cells was assessed by Alizarin Red staining. A total of 110 intersectional DEmRs between patients with osteoporosis and controls from GSE35958 and GSE56815, which were mainly enriched in estrogen, the thyroid hormone signaling pathway, and adherens junctions were identified. A ceRNA network [circ_0070304/miR‑183‑5p/ring finger and CCCH‑type domains 2 (RC3H2)] was then constructed. Circ_0070304 acted as a sponge for miR‑183‑5p and regulated RC3H2 expression. Overexpression of circ_0070304 upregulated ROCK1 and induced osteogenic differentiation. The ceRNA regulatory network that was obtained is expected to be a new target for osteoporosis treatment and to provide new insights into the diagnosis and treatment of osteoporosis in greater depth.", "claims": [{"id": "claim-method", "statement": "A nomogram was built using logistic regression analysis with the rms R package (v3.5.3) (24) based on DEmRs to evaluate the risk assessment of osteoporosis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37326104", "locator": "sections[7] \"Logistic regression analysis\" paragraph 2", "quote": "A nomogram was built using logistic regression analysis with the rms R package (v3.5.3) (24) based on DEmRs to evaluate the risk assessment of osteoporosis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used during the present study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37326104", "locator": "sections[26] \"Availability of data and materials\" paragraph 2", "quote": "The datasets used during the present study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified and experimentally validated the circ_0070304/miR-183-5p/RC3H2 ceRNA network as a regulatory mechanism associated with osteoporosis and osteogenic differentiation.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37326104", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Circ_0070304 acted as a sponge for miR-183-5p and regulated RC3H2 expression."}, {"asset": "source:pmid:37326104", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Overexpression of circ_0070304 upregulated ROCK1 and induced osteogenic differentiation."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified and experimentally validated the circ_0070304/miR-183-5p/RC3H2 ceRNA network as a regulatory mechanism associated with osteoporosis and osteogenic differentiation.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37326104"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Cell culture and transfection Primary human bone marrow mesenchymal stem c", "gy Cо., Ltd. These plasmids (500 ng/ml) were transfected with Lipofectamine 2000 (Invitrogen; Thermo", "activity of circRNA-WT and circRNA-Mut upon transfection with miR-183-5p mimics. (D) Luciferase activ", "rase activity of RC3H2-WT and RC3H2-Mut upon transfection with miR-183-5p mimics. (E) Correlation anal"], "order_basis": "paper_text_order:3051", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.77, "evidence": ["approval no. LFYLLSC20191231-06). BMSCs were cultured in DMEM medium (Hyclone; Cytiva) containing 10%"], "order_basis": "paper_text_order:3359", "id": "assay:2"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["w tissue from patients was collected. In the RT-qPCR results, key DEmRs were all downregulated in", "issue from patients was collected. In the RT-qPCR results, key DEmRs were all downregulated in"], "order_basis": "paper_text_order:5309", "id": "assay:3"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ges in the protein levels of key DEmRs using western blotting. 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