Analysis of mRNA-miRNA interaction network reveals the role of CAFs-derived exosomes in the immune regulation of oral squamous cell carcinoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37365497", "kind": "science-record", "revision": 3, "content_hash": "sha256:2543c1aa4cdae4749336e439aee6f4c6adf63cad557a170c4410d79bfb5bca48", "created_at": "2026-11-01T19:02:06Z", "updated_at": "2026-11-01T22:09:45Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Analysis of mRNA-miRNA interaction network reveals the role of CAFs-derived exosomes in the immune regulation of oral squamous cell carcinoma.", "doi": "10.1186/s12885-023-11028-5", "pmid": "37365497", "authors": ["Wei-Zhou Wang", "Xue Cao", "Li Bian", "Yue Gao", "Ming Yu", "Yi-Ting Li", "Jian-Guo Xu", "Yang-Hao Wang", "He-Feng Yang", "Ding-Yun You", "Yong-Wen He"], "journal": "BMC cancer", "year": 2023, "abstract": "BACKGROUND: Cancer-associated fibroblasts (CAFs) have significant tumor regulatory functions, and CAFs-derived exosomes (CAFs-Exo) released from CAFs play an important role in the progression of oral squamous cell carcinoma (OSCC). However, a lack of comprehensive molecular biological analysis leaves the regulatory mechanisms of CAFs-Exo in OSCC unclear.\nMETHODS: We used platelet derived growth factor-BB (PDGF-BB) to induce the transformation of human oral mucosa fibroblast (hOMF) into CAFs, and extracted exosomes from the supernatant of CAFs and hOMF. We validated the effect of CAFs-Exo on tumor progression by exosomes co-culture with Cal-27 and tumor-forming in nude mice. The cellular and exosomal transcriptomes were sequenced, and immune regulatory genes were screened and validated using mRNA-miRNA interaction network analysis in combination with publicly available databases.\nRESULTS: The results showed that CAFs-Exo had a stronger ability to promote OSCC proliferation and was associated with immunosuppression. We discovered that the presence of immune-related genes in CAFs-Exo may regulate the expression of PIGR, CD81, UACA, and PTTG1IP in Cal-27 by analyzing CAFs-Exo sequencing data and publicly available TCGA data. This may account for the ability of CAFs-Exo to exert immunomodulation and promote OSCC proliferation.\nCONCLUSIONS: CAFs-Exo was found to be involved in tumor immune regulation through hsa-miR-139-5p, ACTR2 and EIF6, while PIGR, CD81, UACA and PTTG1IP may be potentially effective targets for the treatment of OSCC in the future.", "claims": [{"id": "claim-method", "statement": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37365497", "locator": "sections[22] \"ACTR2, EIF6 and hsa-miR-139-5p are associated with survival for OSCC\" paragraph 2", "quote": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets supporting the conclusions of this article are available by contacting corresponding author (Email: youdingyun@qq.com). The Supplementary Material for this article can be found online. All sequencing data have been uploaded to the GEO database with the accession number Series GSE222278.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37365497", "locator": "sections[32] \"Availability of data and materials\" paragraph 2", "quote": "The datasets supporting the conclusions of this article are available by contacting corresponding author (Email: youdingyun@qq.com). The Supplementary Material for this article can be found online. All sequencing data have been uploaded to the GEO database with the accession number Series GSE222278."}], "state": "complete"}, {"id": "claim-target-result", "statement": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001).", "kind": "quantitative", "target": {"value": 0.19, "unit": "hazard ratio", "tolerance": null, "source": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001)."}, "evidence": [{"asset": "source:pmid:37365497", "locator": "sections[22] \"ACTR2, EIF6 and hsa-miR-139-5p are associated with survival for OSCC\" paragraph 2", "quote": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "In a Cox regression analysis of 8 miRNAs showed that hsa-miR-139-5p was significantly associated with cancer survival as a protective factor (Fig. 5C, HR = 0.19, P = 0.001).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37365497"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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