GPX4 is a key ferroptosis biomarker and correlated with immune cell populations and immune checkpoints in childhood sepsis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37443372", "kind": "science-record", "revision": 4, "content_hash": "sha256:2237f170ef2d7d2d9501b10552a6871f391c52c4dc5633b0242e4b7bb778b23f", "created_at": "2026-11-01T19:02:12Z", "updated_at": "2026-11-01T22:09:53Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "GPX4 is a key ferroptosis biomarker and correlated with immune cell populations and immune checkpoints in childhood sepsis.", "doi": "10.1038/s41598-023-32992-9", "pmid": "37443372", "authors": ["Guoxin Qu", "Hui Liu", "Jin Li", "Siyuan Huang", "Nannan Zhao", "Ling Zeng", "Jin Deng"], "journal": "Scientific reports", "year": 2023, "abstract": "Sepsis is the uncontrolled reaction of the body to infection-induced inflammation, which results in life-threatening multiple-organ dysfunction (MODS). Although the research on sepsis has advanced significantly in recent years, its pathophysiology remains entirely unknown. Ferroptosis is a new-fashioned type of programmed cell death that may have an impact on sepsis development. However, the precise mechanism still needs to be explored. In this paper, Four pediatric sepsis datasets [training datasets (GSE26378 and GSE26440) and validation datasets (GSE11755 and GSE11281)] were chosen through the GEO (Gene Expression Omnibus) database, and 63 differentially expressions of ferroptosis-relation-genes (DE-FRGs) were eventually discovered using bioinformatics investigation. Functional annotation was performed using GO and KEGG pathway enrichment analysis. Then, four Core-FRGs (FTH1, GPX4, ACSL1, and ACSL6) were extracted after the construction of the protein-protein interaction (PPI) network and the research of the MCODE module. Consequently, Hub-FRG (GPX4) was found using the validation datasets, and correlation exploration of immunity populations (neutrophils, r = - 0.52; CD8 T-cells, r = 0.43) and immunity checkpoints (CD274, r = - 0.42) was implemented. The usefulness of GPX4 as a marker in sepsis was assessed in a mouse model of sepsis. The findings demonstrate that GPX4 is a crucial biomarker and a new latent immunotherapy target for the prediction and therapy of pediatric sepsis.", "claims": [{"id": "claim-method", "statement": "The receiver operating characteristic curve (ROC) technique in the Python package was executed to analyze hub-FRG diagnostic effectiveness according to the training set and validation set.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37443372", "locator": "sections[9] \"Diagnostic value analysis of hub-FRG in the sepsis\" paragraph 2", "quote": "The receiver operating characteristic curve (ROC) technique in the Python package was executed to analyze hub-FRG diagnostic effectiveness according to the training set and validation set."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37443372", "locator": "sections[44] \"Data availability\" paragraph 2", "quote": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37443372"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE11281"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "blocked", "blocked_on": "method:student_t", "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE11281"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:run_analysis blocked on method:student_t"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37443372", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1038/s41598-023-32992-9", "sha256": "sha256:5c12ee867c85e1fe3bc4b63b65a67514cc17aaa868535a7b55656c1cf38da95f", "bytes": 56759, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1038/s41598-023-32992-9", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10345139", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE11281", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE11281", "version": null, "note": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM284858", "GSM284859", "GSM284860", "GSM284861", "GSM284862", "GSM284863", "GSM284864", "GSM284865", "GSM284866"], "sample_count": 9, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Expression data from human PBMCs treated for 6h with the staphylococcal superantigens SEB and SEI", "resolution_checked_at": "2026-11-01T19:09:46Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE11755", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE11755", "version": null, "note": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM297760", "GSM297761", "GSM297762", "GSM297763", "GSM297764", "GSM297765", "GSM297766", "GSM297767", "GSM297768", "GSM297769", "GSM297770", "GSM297771", "GSM297772", "GSM297773", "GSM297774", "GSM297775", "GSM297776", "GSM297777", "GSM297778", "GSM297779", "GSM297780", "GSM297781", "GSM297782", "GSM297783", "GSM297784", "GSM297785", "GSM297786", "GSM297787", "GSM297788", "GSM297789", "GSM297790", "GSM297791", "GSM297792", "GSM297793", "GSM297794", "GSM297795", "GSM297796", "GSM297797", "GSM297798", "GSM297799", "GSM297800"], "sample_count": 41, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Gene expression profiling in pediatric meningococcal sepsis reveals dynamic changes in NK-cell and cytotoxic molecules", "resolution_checked_at": "2026-11-01T19:09:46Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE26378", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE26378", "version": null, "note": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM647527", "GSM647528", "GSM647529", "GSM647530", "GSM647531", "GSM647532", "GSM647533", "GSM647534", "GSM647535", "GSM647536", "GSM647537", "GSM647538", "GSM647539", "GSM647540", "GSM647541", "GSM647542", "GSM647543", "GSM647544", "GSM647545", "GSM647546", "GSM647547", "GSM647548", "GSM647549", "GSM647550", "GSM647551", "GSM647552", "GSM647553", "GSM647554", "GSM647555", "GSM647556", "GSM647557", "GSM647558", "GSM647559", "GSM647560", "GSM647561", "GSM647562", "GSM647563", "GSM647564", "GSM647565", "GSM647566", "GSM647567", "GSM647568", "GSM647569", "GSM647570", "GSM647571", "GSM647572", "GSM647573", "GSM647574", "GSM647575", "GSM647576", "GSM647577", "GSM647578", "GSM647579", "GSM647580", "GSM647581", "GSM647582", "GSM647583", "GSM647584", "GSM647585", "GSM647586", "GSM647587", "GSM647588", "GSM647589", "GSM647590", "GSM647591", "GSM647592", "GSM647593", "GSM647594", "GSM647595", "GSM647596", "GSM647597", "GSM647598", "GSM647599", "GSM647600", "GSM647601", "GSM647602", "GSM647603", "GSM647604", "GSM647605", "GSM647606", "GSM647607", "GSM647608", "GSM647609", "GSM647610", "GSM647611", "GSM647612", "GSM647613", "GSM647614", "GSM647615", "GSM647616", "GSM647617", "GSM647618", "GSM647619", "GSM647620", "GSM647621", "GSM647622", "GSM647623", "GSM647624", "GSM647625", "GSM647626", "GSM647627", "GSM647628", "GSM647629"], "sample_count": 103, "group_sizes": null, "group_sizes_basis": null, "platform": "570", "taxon": "Homo sapiens", "title": "Expression data from validation cohort of children with septic shock", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}, {"id": "data:geo:GSE26440", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE26440", "version": null, "note": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta).", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Datasets analysed in this study are available from GEO database(http://www.ncbi.nlm.nih. gov/geo/). Open-tier datasets not requiring access approval are available for download via GEO (accession number GSE26378: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26378; accession number GSE26440: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26440; accession number GSE11755: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11755; accession number GSE11281: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11281). 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The FRGs dataset is available for browsing via MSigDb databank (https://www.gsea-msigdb.org/gsea/msigdb/), and the MFRGs dataset is available from Mitocarta 3.0 online data base (https://www.broadinstitute.org/mitocarta)."], "basis": "The paper names GSE26378, GSE26440, GSE11755, GSE11281 in Gene Expression Omnibus (GEO) and the accession resolves in the repository. 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