KNTC1 and MCM2 are the molecular targets of gallbladder cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37480569", "kind": "science-record", "revision": 5, "content_hash": "sha256:67c12a991f80bc05441028a54d382a704bd017604ce5008e81b06a567c4d6a9f", "created_at": "2026-11-01T19:02:15Z", "updated_at": "2026-11-01T22:52:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "KNTC1 and MCM2 are the molecular targets of gallbladder cancer.", "doi": "10.18632/aging.204889", "pmid": "37480569", "authors": ["Wei Jia", "Chao Wang"], "journal": "Aging", "year": 2023, "abstract": "BACKGROUND: Gallbladder carcinoma is a malignant epithelial tumor of gallbladder with a high degree of malignancy. However, relationship between KNTC1 and MCM2 and gallbladder cancer is unclear.\nMETHODS: GSE139682 and GSE202479 were downloaded from gene expression omnibus (GEO). Differentially expressed genes (DEGs) were screened. Functional enrichment analysis and gene set enrichment analysis (GSEA) were performed. Protein-protein interaction (PPI) Network was constructed and analyzed. Gene expression heat map was drawn. Comparative toxicogenomics database (CTD) analysis was performed to find diseases most related to core genes. TargetScan was performed for screening miRNAs that regulated central DEGs.\nRESULTS: 230 DEGs were identified. According to GObp analysis, they were mainly concentrated in regulation of ossification, regulation of spindle microtubule and centromere attachment, cytoskeleton tissue of cortical actin. According to GOcc analysis, they are mainly concentrated in plasma membrane part, cell junction, plasma membrane region and anterior membrane. According to GOmf analysis, they are mainly enriched in protein homodimerization activity, proximal promoter sequence-specific DNA binding and sulfur compound binding. KEGG showed that target genes were mainly enriched in Hippo signal pathway, p53 signal pathway and cancer pathway. KIFC2, TUBG1, RACGAP1, CHMP4C, SFN and MYH11 were identified as core genes. Gene expression heat map showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were highly expressed in gallbladder carcinoma samples. CTD analysis showed that KNTC1, MCM2, CKAP2, RACGAP1, CCNB1 were associated with head and neck squamous cell carcinoma, necrosis, inflammation and hepatomegaly.\nCONCLUSIONS: KNTC1 and MCM2 are highly expressed in gallbladder cancer. Higher expression level correlates with worse prognosis.", "claims": [{"id": "claim-method", "statement": "Probe aggregation and background correction of merge matrix of GSE139682 and GSE202479 using R package “limma”. P value were adjusted using Benjamini-Hochberg method.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37480569", "locator": "sections[14] \"Screening of DEGs\" paragraph 2", "quote": "Probe aggregation and background correction of merge matrix of GSE139682 and GSE202479 using R package “limma”. P value were adjusted using Benjamini-Hochberg method."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37480569", "locator": "sections[21] \"Data availability\" paragraph 2", "quote": "The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "KNTC1 and MCM2 are highly expressed in gallbladder cancer, and higher expression levels correlate with worse prognosis.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37480569", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Conclusions: KNTC1 and MCM2 are highly expressed in gallbladder cancer. Higher expression level correlates with worse prognosis."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "KNTC1 and MCM2 are highly expressed in gallbladder cancer, and higher expression levels correlate with worse prognosis.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37480569"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE139682"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE139682"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37480569", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.18632/aging.204889", "sha256": "sha256:1ea24f6896fd105100fc4a64861021c18a7717e43830aceaa7566963cd4cee79", "bytes": 20039, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.18632/aging.204889", "version": null, "note": "Full article fetched from PMC; 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