Long Non-Coding RNA ZSCAN16-AS1 Promotes the Malignant Progression of Melanoma Through Regulating the miR-503-5p/ARL2 Axis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37483470", "kind": "science-record", "revision": 4, "content_hash": "sha256:f5ef2609fc8bf7183a4b25aa1bcec082055158a794525e7e2a782871b0db2843", "created_at": "2026-11-01T19:02:15Z", "updated_at": "2026-11-01T22:52:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Long Non-Coding RNA ZSCAN16-AS1 Promotes the Malignant Progression of Melanoma Through Regulating the miR-503-5p/ARL2 Axis.", "doi": "10.2147/CCID.S407323", "pmid": "37483470", "authors": ["Yuting Zhao", "Xiangzhou Zhang", "Jie Wang", "Yong Li", "Yitong Wu", "Jisong Liu"], "journal": "Clinical, cosmetic and investigational dermatology", "year": 2023, "abstract": "Background: LncRNA zinc finger and SCAN domain containing 16 antisense RNA 1 (ZSCAN16-AS1), a newly identified lncRNA, has been proven to accelerate hepatocellular carcinoma progression. However, the function and molecular mechanism of ZSCAN16-AS1 in melanoma are still unknown.\nMethods: The level of ZSCAN16-AS1 in melanoma tissues was detected and reported in The Cancer Genome Atlas (TCGA) and GEO#GSE15605. CCK-8, Transwell and flow cytometry assays were used to explore the role of ZSCAN16-AS1 in melanoma cells. Luciferase reporter assays and RNA pull-down assays were used to verify the molecular mechanism of ZSCAN16-AS1.\nResults: Here, we found that ZSCAN16-AS1 expression was increased in melanoma. We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma. ZSCAN16-AS1 exerts its pro-tumour role through sponging of miR-503-5p to liberate ADP-ribosylation factor-like protein 2 (ARL2) mRNA transcripts.\nConclusion: These results demonstrated the role and molecular mechanism of ZSCAN16-AS1 in the occurrence and development of melanoma. Therefore, ZSCAN16-AS1 may be used as a specific biomarker in the diagnosis and treatment of melanoma patients.", "claims": [{"id": "claim-method", "statement": "SPSS13.0 (mean ± SD) was used to evaluate the data, and Student’s t-test (comparison for two groups) and one-way analysis of variance (ANOVA, comparison among multiple groups) was used.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37483470", "locator": "sections[14] \"Statistical Analysis\" paragraph 2", "quote": "SPSS13.0 (mean ± SD) was used to evaluate the data, and Student’s t-test (comparison for two groups) and one-way analysis of variance (ANOVA, comparison among multiple groups) was used."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We used The Cancer Genome Atlas (TCGA, 461 melanoma tissues) and Genotype-Tissue Expression (GTEx, 558 normal skin tissues) database to analyse the expression of ZSCAN16-AS1 through GEPIA (http://gepia.cancer-pku.cn/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37483470", "locator": "sections[3] \"Human Tissue Samples\" paragraph 2", "quote": "We used The Cancer Genome Atlas (TCGA, 461 melanoma tissues) and Genotype-Tissue Expression (GTEx, 558 normal skin tissues) database to analyse the expression of ZSCAN16-AS1 through GEPIA (http://gepia.cancer-pku.cn/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37483470", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37483470"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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