Circ-NFKB1 sponges miR-203a-5p to regulate ERBB4 expression and promotes IL-1β induced chondrocytes apoptosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37491357", "kind": "science-record", "revision": 5, "content_hash": "sha256:287532a1b1c3810a53602c0ea41fe8b1e5cbdb4d5036b3756f9d7831bf899583", "created_at": "2026-11-01T19:02:16Z", "updated_at": "2026-11-01T22:52:23Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Circ-NFKB1 sponges miR-203a-5p to regulate ERBB4 expression and promotes IL-1β induced chondrocytes apoptosis.", "doi": "10.1186/s13018-023-03990-4", "pmid": "37491357", "authors": ["Zhao Wang", "Hongwei Bao", "Jingzhao Hou", "Bin Ju", "Yong Ji"], "journal": "Journal of orthopaedic surgery and research", "year": 2023, "abstract": "BACKGROUND: Osteoarthritis (OA) is a chronic disease of the bones and joints that commonly affects middle-aged and elderly individuals, characterized by the degeneration of articular cartilage and inflammation of the joints. The molecular mechanisms of OA urgently need to be further examined. Our study intended to uncover circ-NFKB1/miR-203a-5p/ERBB4 axis in regulating interleukin-1β (IL-1β) induced chondrocytes apoptosis.\nMETHODS: GSE178724, GSE79258 and GSE169077 were downloaded from Gene Expression Omibus (GEO) database and differentially expressed circRNAs, miRNAs and mRNAs were obtained by R software. Annexin V assay was used to determine cell apoptosis rate. ELISA was further performed to identify the inflammation response. Dual-luciferase reporter gene assay was conducted to examine the combination among circ-NFKB1, miR-203a-5p and ERBB4.\nRESULTS: Our research demonstrated that circ-NFKB1 and ERBB4 were significantly upregulated through bioinformatic analysis. MiR-203a-5p was significantly downregulated through bioinformatic analysis. Silencing of circ-NFKB1 notably inhibited the IL-1β induced chondrocytes apoptosis and upregulated ERBB4 expression. Through prediction on bioinformatics analysis, miR-203a-5p was the target binding circ-NFKB1, and ERBB4 was the potential target of miR-203a-5p. Subsequently, these changes induced by the silencing of circ-NFKB1 were reversed upon addition of pcDNA/ERBB4.\nCONCLUSIONS: Silencing circ-NFKB1 could sponge miR-203a-5p to regulate ERBB4 expression and alleviate OA progression.", "claims": [{"id": "claim-method", "statement": "The microarray data was normalized using Bioconductor's fRMA package in R and subsequently differentially expressed circRNAs, miRNAs and mRNAs were analyzed with the R limma package. The mean comparison of multiple groups was carried out by one-way analysis of variance (ANOVA), while of two different groups was by independent-sample t-tests, performing all the statistical analysis with SPSS 20.0 (IBM Corp., Armonk, NY, USA), with P < 0.05 as a significant difference.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37491357", "locator": "sections[3] \"Candidate lncRNA identification\" paragraph 2", "quote": "The microarray data was normalized using Bioconductor's fRMA package in R and subsequently differentially expressed circRNAs, miRNAs and mRNAs were analyzed with the R limma package."}, {"asset": "source:pmid:37491357", "locator": "sections[15] \"Statistical analysis\" paragraph 2", "quote": "The mean comparison of multiple groups was carried out by one-way analysis of variance (ANOVA), while of two different groups was by independent-sample t-tests, performing all the statistical analysis with SPSS 20.0 (IBM Corp., Armonk, NY, USA), with P < 0.05 as a significant difference."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Not applicable.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37491357", "locator": "sections[29] \"Availability of data and materials\" paragraph 2", "quote": "Not applicable."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Silencing circ-NFKB1 could sponge miR-203a-5p to regulate ERBB4 expression and alleviate OA progression.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37491357", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "Silencing circ-NFKB1 could sponge miR-203a-5p to regulate ERBB4 expression and alleviate OA progression."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Silencing circ-NFKB1 could sponge miR-203a-5p to regulate ERBB4 expression and alleviate OA progression.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37491357"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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METHODS: GSE178724, GSE79258 and GSE169077", ". Annexin V assay was used to determine cell apoptosis rate. ELISA was further performed to identif", "bly inhibited the IL-1β induced chondrocytes apoptosis and upregulated ERBB4 expression. Through pr"], "order_basis": "paper_text_order:55", "id": "assay:1"}, {"role": "data_generation", "definition_id": "elisa", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["y was used to determine cell apoptosis rate. 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