MFNG is an independent prognostic marker for osteosarcoma.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37496053", "kind": "science-record", "revision": 2, "content_hash": "sha256:54641e9ffa7e6c76eacb60753b4b12674a578c35cb5c794c35aff90e994bbe6e", "created_at": "2026-11-01T19:02:16Z", "updated_at": "2026-11-01T21:31:13Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MFNG is an independent prognostic marker for osteosarcoma.", "doi": "10.1186/s40001-023-01139-x", "pmid": "37496053", "authors": ["Yi Gao", "Lili Luo", "Yuxing Qu", "Qi Zhou"], "journal": "European journal of medical research", "year": 2023, "abstract": "BACKGROUND: Osteosarcoma (OS) has been the most common malignancy of the bone in children and adolescents, and the unsatisfactory prognosis of OS sufferers has long been a hard nut. Here, we delved into the markers with a prognostic value for predicting the prognosis of OS patients.\nMETHODS: The messenger RNA (mRNA) sequencing data and clinical data of OS were retrieved from a Gene Expression Omnibus (GEO) dataset (GSE39058). Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan-Meier (K-M) curves and Cox regression analysis (CRA). Later, Gene Ontology (GO) biological process analysis was used in verifying the function of different genes. CCK-8 and cell apoptosis assay were performed to evaluate the function of MFNG in U2OS cells.\nRESULTS: Among the obtained genes, Manic Fringe (MFNG) had the closest relevance to prognosis and clinical traits, thus becoming the research object herein. In light of the expression level of MFNG, patients fell into high- and low-MFNG groups. Patients with elevated MFNG expression had a worse prognosis, according to the survival analysis. It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006). Meanwhile, MFNG expression was linked to gender and tumor recurrence, and it was higher in patients with OS recurrence. Moreover, overexpression of MFNG promoted the cell proliferation and inhibited the cell apoptosis of U2OS cells.\nCONCLUSIONS: The expression level of MFNG negatively correlated with OS progression, and as an independent adverse prognostic factor for disease-free survival in OS patients. Moreover, MFNG regulated the cell proliferation and apoptosis of OS cells.", "claims": [{"id": "claim-method", "statement": "Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan–Meier (K-M) curves and Cox regression analysis (CRA).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37496053", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan–Meier (K-M) curves and Cox regression analysis (CRA)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Not applicable.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37496053", "locator": "sections[18] \"Acknowledgements\" paragraph 2", "quote": "Not applicable."}], "state": "complete"}, {"id": "claim-target-result", "statement": "It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006).", "kind": "quantitative", "target": {"value": 0.006, "unit": "p-value", "tolerance": null, "source": "It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006)."}, "evidence": [{"asset": "source:pmid:37496053", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "It was unveiled by the univariate and multivariate analyses that MFNG expression was an independent adverse prognostic factor for disease-free survival in OS patients (p = 0.006).", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37496053"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE39058"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE39058"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37496053", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s40001-023-01139-x", "sha256": "sha256:da9ae239a42190d45befc96f01b08aef5c290b25bafcd06a169fe487ad65cdbc", "bytes": 29014, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1186/s40001-023-01139-x", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10369729", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE39058", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39058", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE39058", "version": null, "note": "Not applicable.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "Not applicable.", "access_statement_kind": "data_availability_statement", "sample_accessions": ["GSM954459", "GSM954460", "GSM954461", "GSM954462", "GSM954463", "GSM954464", "GSM954465", "GSM954466", "GSM954467", "GSM954468", "GSM954469", "GSM954470", "GSM954471", "GSM954472", "GSM954473", "GSM954474", "GSM954475", "GSM954476", "GSM954477", "GSM954478", "GSM954479", "GSM954480", "GSM954481", "GSM954482", "GSM954483", "GSM954484", "GSM954485", "GSM954486", "GSM954487", "GSM954488", "GSM954489", "GSM954490", "GSM954491", "GSM954492", "GSM954493", "GSM954494", "GSM954495", "GSM954496", "GSM954497", "GSM954498", "GSM954499", "GSM954500", "GSM954501", "GSM954502", "GSM954503", "GSM954504", "GSM954505", "GSM954506", "GSM954507", "GSM954508", "GSM954509", "GSM954510", "GSM954511", "GSM954512", "GSM954513", "GSM954514", "GSM954515", "GSM954516", "GSM954517", "GSM954518", "GSM954519", "GSM954520", "GSM954521", "GSM954522", "GSM954523", "GSM954723", "GSM954724", "GSM954725", "GSM954726", "GSM954727", "GSM954728", "GSM954729", "GSM954730", "GSM954731", "GSM954732", "GSM954733", "GSM954734", "GSM954735", "GSM954736", "GSM954737", "GSM954738", "GSM954739", "GSM954740", "GSM954741", "GSM954742", "GSM954743", "GSM954744", "GSM954745", "GSM954746", "GSM954747", "GSM954748", "GSM954749", "GSM954750", "GSM954751", "GSM954752", "GSM954753", "GSM954754", "GSM954755", "GSM954756", "GSM954757", "GSM954758", "GSM954759", "GSM954760", "GSM954761", "GSM954762", "GSM954763", "GSM954764", "GSM954765", "GSM954766", "GSM954767", "GSM954768", "GSM954769", "GSM954770", "GSM954771", "GSM954772", "GSM954773", "GSM954774", "GSM954790", "GSM954791", "GSM954792", "GSM954793", "GSM954794", "GSM954795", "GSM954796", "GSM954797", "GSM954798", "GSM954799", "GSM954800", "GSM954801", "GSM954802", "GSM954803", "GSM954804", "GSM954805", "GSM954806", "GSM954807", "GSM954808", "GSM954809", "GSM954810", "GSM954811", "GSM954812", "GSM954813", "GSM954814", "GSM954815", "GSM954816", "GSM954817", "GSM954818", "GSM954819", "GSM954820", "GSM954821", "GSM954822", "GSM954823", "GSM954824", "GSM954825", "GSM954826", "GSM954847", "GSM954848", "GSM954849", "GSM954850", "GSM954851", "GSM954852", "GSM954853", "GSM954854", "GSM954855", "GSM954856"], "sample_count": 164, "group_sizes": null, "group_sizes_basis": null, "platform": "15762;14951", "taxon": "Homo sapiens", "title": "microRNA- and mRNA-based studies in paraffin-archived human osteosarcoma specimens reveal profiles with reproducible and independent prognostic value", "resolution_checked_at": "2026-11-01T15:39:54Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE39058 not downloaded", "asset:data:geo:GSE39058 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "cox_proportional_hazards", "label": "Survival analysis", "dsl_operations": ["cox_ph"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan–Meier (K-M) curves and Cox regression analysis (CRA)."}}, {"id": "method-2", "family": "survival_analysis", "specific": "kaplan_meier", "label": "Survival analysis", "dsl_operations": ["survival_km"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 0.7, "band": "medium", "quote": "Kaplan-Meier"}}], "targets": [{"id": "target-published-estimate", "claim_id": "claim-target-result", "operation": "cox_ph", "expected_value": 0.006, "unit": "p-value", "tolerance": null, "state": "complete", "note": "The threshold the paper applied to its own result, as the paper states it. 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CCK-8 and cell apoptosis assay were performed to evaluate the functio", "he cell proliferation and inhibited the cell apoptosis of U2OS cells. CONCLUSIONS: The expression l", "r, MFNG regulated the cell proliferation and apoptosis of OS cells.", "n verifying the function of different genes. CCK-8 and cell apoptosis assay were performed to e"], "order_basis": "paper_text_order:681", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["erformed to evaluate the function of MFNG in U2OS cells. RESULTS: Among the obtained genes, Ma", "feration and inhibited the cell apoptosis of U2OS cells. CONCLUSIONS: The expression level of"], "order_basis": "paper_text_order:763", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["Next, prognosis-related genes (PRGs) were filtered with the aid of Kaplan–Meier (K-M) curves and Cox regression analysis (CRA)."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}