Identification of DUSP7 as an RNA Marker for Prognostic Stratification in Acute Myeloid Leukemia: Evidence from Large Population Cohorts.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37538139", "kind": "science-record", "revision": 3, "content_hash": "sha256:987d0525457f54ca2f4794937fbb695fe10f0b3196235e2996c2308441998f2c", "created_at": "2026-11-01T19:02:19Z", "updated_at": "2026-11-01T22:10:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of DUSP7 as an RNA Marker for Prognostic Stratification in Acute Myeloid Leukemia: Evidence from Large Population Cohorts.", "doi": "10.1155/2023/4348290", "pmid": "37538139", "authors": ["Xin Gao"], "journal": "Genetics research", "year": 2023, "abstract": "Background: The problem of prognostic stratification in acute myeloid leukemia (AML) patients still has limitations.\nMethods: The expression profile data and clinical features of AML patients were obtained from multiple publicly available sources, including GSE71014, TCGA-LAML, and TARGET-AML. Single-cell analysis was performed using the TISCH project. All the analysis was conducted in the R software.\nResults: In our study, three public AML cohorts, GSE71014, TARGET-AML, and TCGA-AML, were selected. Then, we identified the prognosis-related molecules through bioinformatic analysis. Finally, the DUSP7 was noticed as a risk factor for AML patients, which has not been reported previously. Biological enrichment analysis and immune-related analysis were performed to illustrate the role of DUSP7 in AML. Single-cell analysis indicated that the DUSP7 was widely distributed in various cells, especially in monocyte/macrophages and malignant. Following this, a prognosis model based on DUSP7-derived genes was constructed, which showed a good prognosis prediction ability in all cohorts.\nConclusions: Our results preliminarily reveal the role and potential mechanism of DUSP7 in AML, providing direction for future research.", "claims": [{"id": "claim-method", "statement": "Next, the molecules remarkably correlated with AML patients' survival were identified using the univariate Cox regression analysis (P < 0.05). Furthermore, LASSO regression analysis was performed for variable optimization. Ultimately, multivariate Cox regression analysis was used to screen independent prognosis markers and model construction.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37538139", "locator": "sections[5] \"2.3. Prognosis Analysis\" paragraph 2", "quote": "Next, the molecules remarkably correlated with AML patients' survival were identified using the univariate Cox regression analysis (P < 0.05). Furthermore, LASSO regression analysis was performed for variable optimization. Ultimately, multivariate Cox regression analysis was used to screen independent prognosis markers and model construction."}], "state": "complete"}, {"id": "claim-data-access", "statement": "For GSE71014, the expression profile of patients was downloaded from the direct link “Series Matrix File(s)” of the website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71014.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37538139", "locator": "sections[3] \"2.1. Data Collection Process\" paragraph 2", "quote": "For GSE71014, the expression profile of patients was downloaded from the direct link “Series Matrix File(s)” of the website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71014."}], "state": "complete"}, {"id": "claim-target-result", "statement": "High DUSP7 expression was associated with poor prognosis in AML, and a six-gene DUSP7-derived prognosis model showed good predictive ability across the AML cohorts.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37538139", "locator": "sections[12] \"3.2. DUSP7 Is Associated with Poor Prognosis of AML Patients\" paragraph 2", "quote": "KM survival curves in GSE71014, TCGA-LAML, and TARGET-AML cohorts all showed that the AML patients with high DUSP7 expression might have a poor prognosis (Figure 3(d), GSE71014, HR = 2.64, P=0.009; Figure 3(e), TARGET-AML, HR = 1.72, P < 0.001; Figure 3(f), TCGA-LAML, HR = 2.27, P < 0.001)."}, {"asset": "source:pmid:37538139", "locator": "sections[15] \"3.5. Establishment of a Prognosis Model Based on the DUSP7-Derived Molecules\" paragraph 2", "quote": "KM survival curves indicated that high-risk patients might have a worse prognosis (Figure 8(b), HR = 3.70, P < 0.001). ROC curves indicated that the 1-year AUC = 0.809, 3-year AUC = 0.851, and 5-year AUC = 0.935 (Figures 8(c)–8(e))."}, {"asset": "source:pmid:37538139", "locator": "sections[15] \"3.5. Establishment of a Prognosis Model Based on the DUSP7-Derived Molecules\" paragraph 2", "quote": "In the validation cohorts (GSE71014 and TARGET-AML), our model also showed a good prognosis prediction ability (Figure 8(f), HR = 2.34, P=0.017, 1-year AUC = 0.687, 3-year AUC = 0.707, 5-year AUC = 0.897; Figure 8(g), HR = 2.57, P < 0.001, 1-year AUC = 0.677, 3-year AUC = 0.696, 5-year AUC = 0.686)."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "High DUSP7 expression was associated with poor prognosis in AML, and a six-gene DUSP7-derived prognosis model showed good predictive ability across the AML cohorts.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37538139"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE71014"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE71014"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:37538139", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1155/2023/4348290", "sha256": "sha256:7b52932ac04ac114a16f7b6aadc3185d3689d45b79b421d4048f2f13d3ef164a", "bytes": 20342, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.1155/2023/4348290", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC10396553", "source": "europe_pmc_fulltext", "coverage": "whole_body_without_references", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE71014", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71014", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE71014", "version": null, "note": "For GSE71014, the expression profile of patients was downloaded from the direct link “Series Matrix File(s)” of the website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71014.", "structure": {"repository": "GEO", "access_route": "GEO accession URL", "access_statement_verbatim": "For GSE71014, the expression profile of patients was downloaded from the direct link “Series Matrix File(s)” of the website https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE71014.", "access_statement_kind": "repository_route_statement", "sample_accessions": ["GSM1825455", "GSM1825456", "GSM1825457", "GSM1825458", "GSM1825459", "GSM1825460", "GSM1825461", "GSM1825462", "GSM1825463", "GSM1825464", "GSM1825465", "GSM1825466", "GSM1825467", "GSM1825468", "GSM1825469", "GSM1825470", "GSM1825471", "GSM1825472", "GSM1825473", "GSM1825474", "GSM1825475", "GSM1825476", "GSM1825477", "GSM1825478", "GSM1825479", "GSM1825480", "GSM1825481", "GSM1825482", "GSM1825483", "GSM1825484", "GSM1825485", "GSM1825486", "GSM1825487", "GSM1825488", "GSM1825489", "GSM1825490", "GSM1825491", "GSM1825492", "GSM1825493", "GSM1825494", "GSM1825495", "GSM1825496", "GSM1825497", "GSM1825498", "GSM1825499", "GSM1825500", "GSM1825501", "GSM1825502", "GSM1825503", "GSM1825504", "GSM1825505", "GSM1825506", "GSM1825507", "GSM1825508", "GSM1825509", "GSM1825510", "GSM1825511", "GSM1825512", "GSM1825513", "GSM1825514", "GSM1825515", "GSM1825516", "GSM1825517", "GSM1825518", "GSM1825519", "GSM1825520", "GSM1825521", "GSM1825522", "GSM1825523", "GSM1825524", "GSM1825525", "GSM1825526", "GSM1825527", "GSM1825528", "GSM1825529", "GSM1825530", "GSM1825531", "GSM1825532", "GSM1825533", "GSM1825534", "GSM1825535", "GSM1825536", "GSM1825537", "GSM1825538", "GSM1825539", "GSM1825540", "GSM1825541", "GSM1825542", "GSM1825543", "GSM1825544", "GSM1825545", "GSM1825546", "GSM1825547", "GSM1825548", "GSM1825549", "GSM1825550", "GSM1825551", "GSM1825552", "GSM1825553", "GSM1825554", "GSM1825555", "GSM1825556", "GSM1825557", "GSM1825558"], "sample_count": 104, "group_sizes": null, "group_sizes_basis": null, "platform": "10558", "taxon": "Homo sapiens", "title": "An mRNA Expression Signature for Prognostication in De Novo Acute Myeloid Leukemia Patients with Normal Karyotype", "resolution_checked_at": "2026-11-01T15:39:55Z", "resolution_check": "identifier_resolution_only_no_download_no_licence_read", "downloaded": false, "licence_read": false}}], "gaps": ["asset:data:geo:GSE71014 not downloaded", "asset:data:geo:GSE71014 licence not read"]}, "analysis": {"state": "complete", "methods": [{"id": "method-1", "family": "survival_analysis", "specific": "cox_proportional_hazards", "label": "Survival analysis", "dsl_operations": ["cox_ph"], "missing_operations": [], "expressible": true, "parameters": {}, "data_shapes": ["time_to_event_with_censoring"], "evidence": {"score": 1.0, "band": "verbatim", "quote": "Next, the molecules remarkably correlated with AML patients' survival were identified using the univariate Cox regression analysis (P < 0.05). 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