KIF11 serves as a cell cycle mediator in childhood acute lymphoblastic leukemia.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37656243", "kind": "science-record", "revision": 3, "content_hash": "sha256:e20dc90470270e5a78fd3c0231d4c08d20e8d5eb9321f24643b3189456f13534", "created_at": "2026-11-01T19:02:26Z", "updated_at": "2026-11-01T21:31:26Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "KIF11 serves as a cell cycle mediator in childhood acute lymphoblastic leukemia.", "doi": "10.1007/s00432-023-05240-w", "pmid": "37656243", "authors": ["Liwen Zhu", "Chuqin Chen", "Meiyun Kang", "Xiaopeng Ma", "Xiaoyan Sun", "Yao Xue", "Yongjun Fang"], "journal": "Journal of cancer research and clinical oncology", "year": 2023, "abstract": "OBJECTIVE: To identify key gene in childhood acute lymphoblastic leukemia (ALL) through weighted gene co-expression network analysis (WGCNA), and their enriched biological functions and signaling pathways.\nMETHODS: Array data of the GSE73578 dataset, involving 46 childhood ALL samples, were acquired from the Gene Expression Omnibus (GEO) database. Hub modules associated with childhood ALL were screened out by WGCNA. Enriched biological functions and signaling pathways were then identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Hub genes were selected by overlapping those between down-regulated genes in GSE73578, GSE4698 and the hub module. Guilt by association (GBA) was adopted to verify the function of the identified KIF11 gene and to predict its target genes. Regulatory effects of KIF11 on the proliferation and cell cycle progression of ALL in vitro were determined by cytological experiments.\nRESULTS: WGCNA showed that the yellow module was the most relevant to childhood ALL treatment, containing 698 genes that were enriched in cell division, mitotic nuclear division, DNA replication and DNA repair, cell cycle, DNA replication and the P53 signaling pathway. The KIF11 gene was screened out and predicted as a cell cycle mediator in childhood ALL. Knockdown of KIF11 in ALL cells inhibited cell proliferation and arrested cell cycle progression in G2/M phase.\nCONCLUSIONS: The KIF11 gene is critical in the treatment process of childhood ALL, which is a promising therapeutic target for childhood ALL.", "claims": [{"id": "claim-method", "statement": "Hub modules associated with childhood ALL were screened out by WGCNA.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37656243", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "Hub modules associated with childhood ALL were screened out by WGCNA."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37656243", "locator": "sections[31] \"notes\" paragraph 1", "quote": "The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "WGCNA showed that the yellow module was the most relevant to childhood ALL treatment, containing 698 genes that were enriched in cell division, mitotic nuclear division, DNA replication and DNA repair, cell cycle, DNA replication and the P53 signaling pathway.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37656243", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "WGCNA showed that the yellow module was the most relevant to childhood ALL treatment, containing 698 genes that were enriched in cell division, mitotic nuclear division, DNA replication and DNA repair, cell cycle, DNA replication and the P53 signaling pathway."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "WGCNA showed that the yellow module was the most relevant to childhood ALL treatment, containing 698 genes that were enriched in cell division, mitotic nuclear division, DNA replication and DNA repair, cell cycle, DNA replication and the P53 signaling pathway.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37656243"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Knockdown of KIF11 in ALL cells inhibited cell prolife", "rdians of all participants. Cell culture and transfection Jurkat (T line), Nalm-6 (B line), and 6T-CEM", ", TTGAATAAGCCTGAAGTGAAT. Briefly, cells were transfected with plasmids for 72 h, and then incubated w", "4 μg/mL puromycin for selection. Effectively transfected cells were assessed by fluorescence staining"], "order_basis": "paper_text_order:1338", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["of Chinese Academy of Medical Sciences) were cultured in RPMI 1640 (Gibco, NY, USA) containing 10% fe", "Committee, Chinese Academy of Sciences) were cultured in RPMI 1640 containing 20% FBS and 10% penicil"], "order_basis": "paper_text_order:3730", "id": "assay:2"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.93, "evidence": ["ent of transfection efficacy via qRT-PCR and Western blot. Statistical analysis Statistical analysis w"], "order_basis": "paper_text_order:4549", "id": "assay:3"}]}