A 3D printed plastic frame deeply impacts yeast cell growth.

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In this work, we present a polylactic acid (PLA) 3D printed matrix that dramatically enhances yeast growth when embedded in liquid media compared to equivalent static cultures, and changes yeast expression patterns at the proteome level (data are available via ProteomeXchange with identifier PXD043759). Moreover, differences in sugar assimilation and ethanol production, as the main product of alcoholic fermentation, are observed. Our results suggest that these matrixes may be useful for a vast range of biotechnological applications based on yeast fermentation.", "claims": [{"id": "claim-method", "statement": "One-way ANOVA followed by Tukey’s multiple comparisons test was performed using GraphPad Prism version 9.0.0 for macOS, GraphPad Software, San Diego, California United States, www.graphpad.com, for both OD600 and metabolite measurements. 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