Regulation of ferroptosis-related genes in CD8+ NKT cells and classical monocytes may affect the immunotherapy response after combined treatment in triple negative breast cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37830388", "kind": "science-record", "revision": 3, "content_hash": "sha256:5baeab247b25c949daf833dd09c262b9f86415a8ab2882df5e7a85a9a0ddaa00", "created_at": "2026-11-01T19:02:41Z", "updated_at": "2026-11-01T22:10:27Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Regulation of ferroptosis-related genes in CD8+ NKT cells and classical monocytes may affect the immunotherapy response after combined treatment in triple negative breast cancer.", "doi": "10.1111/1759-7714.15128", "pmid": "37830388", "authors": ["Zheming Liu", "Songjiang He", "Zhou Huang", "Jiahui Liu", "Yiping Gong", "Yi Yao", "Xue Zhang"], "journal": "Thoracic cancer", "year": 2023, "abstract": "BACKGROUND: Drug resistance has led to the failure of immunotherapy in triple negative breast cancer patients. Here we aimed to explore the mechanisms of drug resistance in patients in order to enhance their response to immunotherapy.\nMETHODS: We downloaded publicly available single-cell RNA-sequencing data of peripheral blood mononuclear cells from patients after treatment to investigate the possible mechanisms of drug resistance. The publicly available TCGA transcriptomic data and somatic mutation data were used for further validation. In this study, a series of bioinformatics and machine learning methods were employed.\nRESULTS: We identified the vital roles of CD8+ NKT cells and classical monocytes in the immunotherapy response of triple-negative breast cancer patients. The proportion of these cell types was significantly increased in group partial response. We also found that downregulation of ferroptosis-related genes regulates the immune pathway. The analysis of scRNA data and TCGA transcriptomic data presented that DUSP1 may play a crucial role in immunotherapy resistance.\nCONCLUSION: Overall, the composition of the tumor microenvironment affects the immunotherapy response of patients, and DUSP1 may be a potential target for overcoming drug resistance.", "claims": [{"id": "claim-method", "statement": "The R package Survival was used to estimate the survival differences between the two groups. The Kaplan–Meier method was used to plot survival curves, and the log‐rank test was used to assess the statistical differences in prognoses between different groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37830388", "locator": "sections[6] \"Survival analysis\" paragraph 2", "quote": "The R package Survival was used to estimate the survival differences between the two groups. The Kaplan–Meier method was used to plot survival curves, and the log‐rank test was used to assess the statistical differences in prognoses between different groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "We collected peripheral blood mononuclear cell (PBMC) samples from 22 advanced TNBC patients (4 partial response [PR], 7 stable disease [SD], and 11 pretreatment [Pre]) that were publicly available in the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37830388", "locator": "sections[3] \"Data acquisition and preprocessing\" paragraph 2", "quote": "We collected peripheral blood mononuclear cell (PBMC) samples from 22 advanced TNBC patients (4 partial response [PR], 7 stable disease [SD], and 11 pretreatment [Pre]) that were publicly available in the Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The analysis of scRNA data and TCGA transcriptomic data presented that DUSP1 may play a crucial role in immunotherapy resistance.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37830388", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "The analysis of scRNA data and TCGA transcriptomic data presented that DUSP1 may play a crucial role in immunotherapy resistance."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The analysis of scRNA data and TCGA transcriptomic data presented that DUSP1 may play a crucial role in immunotherapy resistance.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37830388"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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