[Identification of Peripheral Blood GZMK + CD8 + T Cells As Biomarkers of Alzheimer's Disease Based on Single-Cell Transcriptome].

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37866940", "kind": "science-record", "revision": 1, "content_hash": "sha256:da811b681d050118b0b1bffb0e2689a674706493ec0a31f9903f968153d80372", "created_at": "2026-11-01T19:02:43Z", "updated_at": "2026-11-01T19:02:43Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "[Identification of Peripheral Blood GZMK + CD8 + T Cells As Biomarkers of Alzheimer's Disease Based on Single-Cell Transcriptome].", "doi": "10.12182/20230960107", "pmid": "37866940", "authors": ["Tingting Duan", "Jinyu Chu", "Feifei Hu"], "journal": "Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition", "year": 2023, "abstract": "Objective: Based on single-cell RNA sequencing (scRNA-seq) to explore immune characteristics in the peripheral blood of patients with Alzheimer's disease (AD) as biomarkers.\nMethods: GSE168522, the scRNA-seq dataset of AD peripheral blood immune cells, was downloaded from the Gene Expression Omnibus (GEO) database and was analyzed in the RAD-Blood web server (http://www.bioinform.cn/RAD-Blood/). The changes in blood cell composition in AD patients were analyzed. The abnormal communications between different types of cells in AD patients were investigated by the CellChat R package.\nResults: There were two kinds of CD8 + T cells in the blood of AD patients and healthy individuals, one of which highly expressed granzyme K ( GZMK) (false discovery rate [FDR]<0.05), and the other highly expressed GZMA, GZMB, and GZMH (FDR<0.05). In the blood of AD patients, the content of GZMK + CD8 + T cells was increased by 32.9% ( P=5.15E-21), their interactions with other cell types were increased, and they might be associated with AD through the abnormal signal transduction of major histocompatibility complex class Ⅰ (MHC-Ⅰ). Erythrocyte provided the main ligands, that are, human leukocyte antigen (HLA) class Ⅰ molecules, including HLA- A, HLA- B, HLA- C, and HLA- E, for the abnormal MHC-Ⅰ signaling pathway of GZMK + CD8 + T cells. The RESISTIN signaling pathway was specifically enriched in the blood of AD patients.\nConclusion: The increased content of peripheral blood GZMK + CD8 + T cells, the increased interaction between GZMK + CD8 + T cells and erythrocytes, and the enhanced RESISTIN pathway are potential blood biomarkers of AD.", "claims": [{"id": "claim-data-access", "statement": "外周血单细胞转录组数据来源于GEO数据库的GSE168522[6]数据集，该数据集提取并纯化了4例（GSM5145403、GSM5145404、GSM5145405和GSM5145406）AD患者和2名（GSM5145401和GSM5145402）正常人的外周血单核细胞，并使用10× Genomics Chromium测序平台进行scRNA-seq。", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37866940", "locator": "sections[5] \"数据收集\" paragraph 2", "quote": "外周血单细胞转录组数据来源于GEO数据库的GSE168522[6]数据集，该数据集提取并纯化了4例（GSM5145403、GSM5145404、GSM5145405和GSM5145406）AD患者和2名（GSM5145401和GSM5145402）正常人的外周血单核细胞，并使用10× Genomics Chromium测序平台进行scRNA-seq。"}], "state": "complete"}, {"id": "claim-target-result", "statement": "GZMK+ CD8+ T细胞在AD患者血液中含量升高32.9%（P=5.15E-21），与其他细胞类型的交互作用增加，并可能通过主要组织相容性复合体Ⅰ类（major histocompatibility complex class Ⅰ, MHC-Ⅰ）信号转导异常与AD关联，红细胞为GZMK+ CD8+ T细胞MHC-Ⅰ信号通路异常提供了主要配体，即人类白细胞抗原（human leukocyte antigen, HLA）Ⅰ类分子（HLA-A、HLA-B、HLA-C和HLA-E）。", "kind": "quantitative", "target": {"value": 32.9, "unit": "per cent", "tolerance": null, "source": "GZMK+ CD8+ T细胞在AD患者血液中含量升高32.9%（P=5.15E-21），与其他细胞类型的交互作用增加，并可能通过主要组织相容性复合体Ⅰ类（major histocompatibility complex class Ⅰ, MHC-Ⅰ）信号转导异常与AD关联，红细胞为GZMK+ CD8+ T细胞MHC-Ⅰ信号通路异常提供了主要配体，即人类白细胞抗原（human leukocyte antigen, HLA）Ⅰ类分子（HLA-A、HLA-B、HLA-C和HLA-E）。"}, "evidence": [{"asset": "source:pmid:37866940", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "GZMK+ CD8+ T细胞在AD患者血液中含量升高32.9%（P=5.15E-21），与其他细胞类型的交互作用增加，并可能通过主要组织相容性复合体Ⅰ类（major histocompatibility complex class Ⅰ, MHC-Ⅰ）信号转导异常与AD关联，红细胞为GZMK+ CD8+ T细胞MHC-Ⅰ信号通路异常提供了主要配体，即人类白细胞抗原（human leukocyte antigen, HLA）Ⅰ类分子（HLA-A、HLA-B、HLA-C和HLA-E）。"}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "GZMK+ CD8+ T细胞在AD患者血液中含量升高32.9%（P=5.15E-21），与其他细胞类型的交互作用增加，并可能通过主要组织相容性复合体Ⅰ类（major histocompatibility complex class Ⅰ, MHC-Ⅰ）信号转导异常与AD关联，红细胞为GZMK+ CD8+ T细胞MHC-Ⅰ信号通路异常提供了主要配体，即人类白细胞抗原（human leukocyte antigen, HLA）Ⅰ类分子（HLA-A、HLA-B、HLA-C和HLA-E）。", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37866940"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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