Management of ulcerative colitis by dichloroacetate: Impact on NFATC1/NLRP3/IL1B signaling based on bioinformatics analysis combined with in vivo experimental verification.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37902927", "kind": "science-record", "revision": 5, "content_hash": "sha256:5d4732f3c9b2882c3d4512e2fcdefb722083684193144995713455a267fcedf7", "created_at": "2026-11-01T19:02:46Z", "updated_at": "2026-11-01T22:52:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Management of ulcerative colitis by dichloroacetate: Impact on NFATC1/NLRP3/IL1B signaling based on bioinformatics analysis combined with in vivo experimental verification.", "doi": "10.1007/s10787-023-01362-2", "pmid": "37902927", "authors": ["Esraa Abdel-Nassir Abdel-Razek", "Heba M Mahmoud", "Amany A Azouz"], "journal": "Inflammopharmacology", "year": 2024, "abstract": "The inflammatory response in ulcerative colitis (UC) could be relieved by the conventional immunomodulatory agents; 5-aminosalicylic acid, corticosteroids, or azathioprine. However, the low remission rates and the intolerance to these agents necessitate investigation of gene expression signature in UC that could influence the therapeutic efficacy of drugs, as well as the interference with persistence genes by novel therapeutic option. Three microarray datasets (GSE66407, GSE38713 and GSE14580) from the NCBI-GEO database were utilized. Differentially expressed genes between samples of patients with UC and healthy ones were analyzed using R software. In addition, in vivo study using oxazolone-induced UC in BALB/c mice was carried out to investigate the proposed therapeutic efficacy of dichloroacetate (DCA). The bioinformatics analysis revealed the persistence of NLRP3, NFATC1, and IL1B in UC despite treatment with common therapeutic agents. DCA administration to oxazolone-treated mice showed remarkable interference with those persistence genes. Western blotting analysis for NLRP3, NFATC1, nuclear/total NF-κB, and cleaved caspase-1 revealed the ability of DCA to reduce the expression levels of these proteins in oxazolone-treated mice. Additionally, the inflammatory cytokines IL-1β and IL-13 were reduced in colonic tissue by DCA treatment. The therapeutic efficacy of DCA was further confirmed by the apparent reduction in histopathological scoring, disease activity index, and the normalization of colon length. Therefore, DCA could be suggested as a novel and promising therapeutic option in UC based on its ability to interfere with the persistence of NFATC1/NLRP3/IL1B signaling. That merits further safety/toxicological pre-clinical assessment and update of bioavailability/metabolism data prior to clinical investigation.", "claims": [{"id": "claim-method", "statement": "Significant difference between groups was investigated using one-way analysis of variance (ANOVA) followed by Tukey’s.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37902927", "locator": "sections[11] \"Statistical analysis\" paragraph 2", "quote": "Significant difference between groups was investigated using one-way analysis of variance (ANOVA) followed by Tukey’s."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Enquiries about data availability should be directed to the authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37902927", "locator": "sections[25] \"notes\" paragraph 1", "quote": "Enquiries about data availability should be directed to the authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "DCA could be suggested as a novel and promising therapeutic option in UC based on its ability to interfere with the persistence of NFATC1/NLRP3/IL1B signaling.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37902927", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Therefore, DCA could be suggested as a novel and promising therapeutic option in UC based on its ability to interfere with the persistence of NFATC1/NLRP3/IL1B signaling."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "DCA could be suggested as a novel and promising therapeutic option in UC based on its ability to interfere with the persistence of NFATC1/NLRP3/IL1B signaling.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37902927"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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