miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37904207", "kind": "science-record", "revision": 4, "content_hash": "sha256:4aa1c3688fb815538fa51a1aa8ff52326175ebac87b5be9135cfe3681aa4c6a2", "created_at": "2026-11-01T19:02:46Z", "updated_at": "2026-11-01T22:52:59Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4.", "doi": "10.1186/s13018-023-04321-3", "pmid": "37904207", "authors": ["Jianwei Liang", "Dandan Bao", "Zhan Ye", "Binhao Cao", "Guojun Jin", "Zhenyu Lu", "Jianjun Chen"], "journal": "Journal of orthopaedic surgery and research", "year": 2023, "abstract": "BACKGROUND: Osteosarcoma (OS) is a highly invasive primary malignancy of the bone that is common in children and adolescents. MicroRNAs (miRNAs) are novel diagnostic and predictive biomarkers for cancers. The miRNA miR-3195 is aberrantly expressed in multiple types of tumors. However, the expression levels and biological functions of miR-3195 in OS remain unclear.\nMETHODS: Two Gene Expression Omnibus (GEO) datasets (GSE69470 and GSE16088) were used to analyze differentially expressed miRNAs and mRNAs in osteosarcoma cell lines and OS tissues. Quantitative RT-PCR was used to detect the expression levels of miR-3195 and the SRY-box transcription factor 4 (SOX4) mRNA in OS tissues and cell lines. The relationship between miR-3195 and the 3'-upstream region (3'-UTR) in the SOX4 mRNA (predicted through bioinformatics) was analyzed using Pearson's correlation analysis and confirmed by a dual-luciferase reporter gene experiment. Cell counting kit-8 assays, colony formation assays, flow cytometry, wound healing assays, transwell assays, and western blotting were performed to explore the effects of miR-3195 levels on SOX4 affected OS cell biological behavior.\nRESULTS: Our results revealed that miR-3195 was the most down-regulated miRNA and SOX4 was the most up-regulated mRNA by Bioinformatic analysis. It was further confirmed miR-3195 had low expression, and SOX4 had high expression levels in clinical OS tissue samples; the expression levels of both genes were negatively correlated with each other in OS tissues. Overexpression of miR-3195 in OS cell lines significantly inhibited cell proliferation, migration, and invasiveness, while promoting apoptosis; all these effects were reversed by increasing SOX4 expression levels. We also found that miR-3195 could directly bind with the SOX4 gene and down-regulate SOX4 expression.\nCONCLUSIONS: miR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene. Thus, the miR-3195/SOX4 signaling may be a novel therapeutic target in OS treatment.", "claims": [{"id": "claim-method", "statement": "Student's t-test was used for comparisons between two groups, and one-way or two-way with post hoc analysis of variance was used for comparisons among multiple groups.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37904207", "locator": "sections[13] \"Statistical analysis\" paragraph 2", "quote": "Student's t-test was used for comparisons between two groups, and one-way or two-way with post hoc analysis of variance was used for comparisons among multiple groups."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The data supporting the findings of this study are available from the corresponding author upon reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37904207", "locator": "sections[25] \"Availability of data and materials\" paragraph 2", "quote": "The data supporting the findings of this study are available from the corresponding author upon reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "miR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37904207", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "miR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "miR-3195 can modulate proliferation, migration, invasiveness, and apoptosis in OS cells by regulating the SOX4 gene.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37904207"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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