Bone Marrow Mesenchymal Stem Cells Release miR-378a-5p-Carried Extracellular Vesicles to Alleviate Rheumatoid Arthritis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37926093", "kind": "science-record", "revision": 5, "content_hash": "sha256:298d354d9aab30c30473035bd93ecf9a1c6ad33b8f167243590487f1c12fa124", "created_at": "2026-11-01T19:02:48Z", "updated_at": "2026-11-01T22:53:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Bone Marrow Mesenchymal Stem Cells Release miR-378a-5p-Carried Extracellular Vesicles to Alleviate Rheumatoid Arthritis.", "doi": "10.1159/000534830", "pmid": "37926093", "authors": ["Yaqin Zhang", "Ziying Jiao", "Shanshan Wang"], "journal": "Journal of innate immunity", "year": 2023, "abstract": "This study investigates whether bone marrow mesenchymal stem cell (BMSC)-derived extracellular vesicles (EVs) can affect rheumatoid arthritis (RA) by delivering microRNA (miR)-378a-5p to regulate the interferon regulatory factor 1/signal transducer and transcription 1 (IRF1/STAT1) axis. We identified RA-associated miRNAs using the GEO microarray dataset GSE121894. We found the most important miRNAs in RA synovial tissues using RT-qPCR. BMSC-derived EVs were ultracentrifuged and cocultured with human synovial microvascular endothelial cells (HSMECs) in vitro. Dual-luciferase and RNA immunoprecipitation studies examined miR-378a-5p's specific binding to IRF1. We also measured angiogenesis, migration, and proliferation using CCK-8, Transwell, and tube formation assays. Collagen-induced arthritis (CIA) mice models were created by inducing arthritis and scoring it. RA synovial tissues had low miR-378a-5p expression, whereas BMSC-derived EVs had high levels. The transfer of miR-378a-5p by BMSC-derived EVs to HSMECs boosted proliferation, migration, and angiogenesis. miR-378a-5p inhibited IRF1. MiR-378a-5p-containing BMSC-derived EVs decreased STAT1 phosphorylation and HSMEC IRF1 expression. EVs with miR-378a-5p mimic promoted HSMEC proliferation, migration, and angiogenesis, whereas dexmedetomidine inhibited STAT1 phosphorylation. In CIA mice, BMSC-derived EVs containing miR-378a-5p enhanced synovial vascular remodeling and histopathology. Thus, miR-378a-5p from BMSC-derived EVs promotes HSMEC proliferation, migration, and angiogenesis, inactivating the IRF1/STAT1 axis and preventing RA.", "claims": [{"id": "claim-method", "statement": "Data between groups were analyzed using an unpaired t test. One-way ANOVA was used to compare data among multiple groups, followed by Tukey’s post hoc tests. Data between groups at different time points were compared using repeated measures ANOVA combined with Bonferroni post hoc tests.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37926093", "locator": "sections[22] \"Statistical Analysis\" paragraph 2", "quote": "Data between groups were analyzed using an unpaired t test. 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