Plasma-Derived Exosome Proteins as Novel Diagnostic and Prognostic Biomarkers in Neuroblastoma Patients.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37947594", "kind": "science-record", "revision": 4, "content_hash": "sha256:1833df4d3ec3e61cbcdc72b1e180731bfc7504df30a747f3265c072e4189ed8f", "created_at": "2026-11-01T19:02:50Z", "updated_at": "2026-11-01T22:10:37Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Plasma-Derived Exosome Proteins as Novel Diagnostic and Prognostic Biomarkers in Neuroblastoma Patients.", "doi": "10.3390/cells12212516", "pmid": "37947594", "authors": ["Martina Morini", "Federica Raggi", "Martina Bartolucci", "Andrea Petretto", "Martina Ardito", "Chiara Rossi", "Daniela Segalerba", "Alberto Garaventa", "Alessandra Eva", "Davide Cangelosi", "Maria Carla Bosco"], "journal": "Cells", "year": 2023, "abstract": "Neuroblastoma (NB) is the most common extracranial solid tumor during infancy, causing up to 10% of mortality in children; thus, identifying novel early and accurate diagnostic and prognostic biomarkers is mandatory. NB-derived exosomes carry proteins (Exo-prots) reflecting the status of the tumor cell of origin. The purpose of this study was to characterize, for the first time, the Exo-prots specifically expressed in NB patients associated with tumor phenotype and disease stage. We isolated exosomes from plasma specimens of 24 HR-NB patients and 24 low-risk (LR-NB) patients at diagnosis and of 24 age-matched healthy controls (CTRL). Exo-prot expression was measured by liquid chromatography-mass spectrometry. The data are available via ProteomeXchange (PXD042422). The NB patients had a different Exo-prot expression profile compared to the CTRL. The deregulated Exo-prots in the NB specimens acted mainly in the tumor-associated pathways. The HR-NB patients showed a different Exo-prot expression profile compared to the LR-NB patients, with the modulation of proteins involved in cell migration, proliferation and metastasis. NCAM, NCL, LUM and VASP demonstrated a diagnostic value in discriminating the NB patients from the CTRL; meanwhile, MYH9, FN1, CALR, AKAP12 and LTBP1 were able to differentiate between the HR-NB and LR-NB patients with high accuracy. Therefore, Exo-prots contribute to NB tumor development and to the aggressive metastatic NB phenotype.", "claims": [{"id": "claim-method", "statement": "Receiver operating characteristic (ROC) curves were plotted to visually display the discriminating power of each significantly modulated Exo-prot across groups. Exo-prot expression was used to generate ROC curves.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37947594", "locator": "sections[7] \"2.5. Statistical Analysis\" paragraph 2", "quote": "Receiver operating characteristic (ROC) curves were plotted to visually display the discriminating power of each significantly modulated Exo-prot across groups. Exo-prot expression was used to generate ROC curves."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The proteomic data have been deposited to the ProteomeXchange Consortium via the PRIDE [61] partner repository with the dataset identifier PXD042422.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37947594", "locator": "sections[24] \"Data Availability Statement\" paragraph 2", "quote": "The proteomic data have been deposited to the ProteomeXchange Consortium via the PRIDE [61] partner repository with the dataset identifier PXD042422."}], "state": "complete"}, {"id": "claim-target-result", "statement": "NCAM, NCL, LUM and VASP demonstrated a diagnostic value in discriminating the NB patients from the CTRL; meanwhile, MYH9, FN1, CALR, AKAP12 and LTBP1 were able to differentiate between the HR-NB and LR-NB patients with high accuracy.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37947594", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "NCAM, NCL, LUM and VASP demonstrated a diagnostic value in discriminating the NB patients from the CTRL; meanwhile, MYH9, FN1, CALR, AKAP12 and LTBP1 were able to differentiate between the HR-NB and LR-NB patients with high accuracy."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "NCAM, NCL, LUM and VASP demonstrated a diagnostic value in discriminating the NB patients from the CTRL; meanwhile, MYH9, FN1, CALR, AKAP12 and LTBP1 were able to differentiate between the HR-NB and LR-NB patients with high accuracy.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37947594"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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