Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37953800", "kind": "science-record", "revision": 4, "content_hash": "sha256:66ea701dceea1c478dd087df44915065e8e4085b981c846ae45f284642f3f662", "created_at": "2026-11-01T19:02:51Z", "updated_at": "2026-11-01T22:10:38Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Unravelling the sexual developmental biology of Cystoisospora suis, a model for comparative coccidian parasite studies.", "doi": "10.3389/fcimb.2023.1271731", "pmid": "37953800", "authors": ["Teresa Cruz-Bustos", "Marlies Dolezal", "Anna Sophia Feix", "Bärbel Ruttkowski", "Karin Hummel", "Ebrahim Razzazi-Fazeli", "Anja Joachim"], "journal": "Frontiers in cellular and infection microbiology", "year": 2023, "abstract": "Introduction: The apicomplexan parasite Cystoisospora suis has global significance as an enteropathogen of suckling piglets. Its intricate life cycle entails a transition from an asexual phase to sexual development, ultimately leading to the formation of transmissible oocysts.\nMethods: To advance our understanding of the parasite's cellular development, we complemented previous transcriptome studies by delving into the proteome profiles at five distinct time points of in vitro cultivation through LC/MS-MS analysis.\nResults: A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed. Data are available via ProteomeXchange with identifier PXD045050. We performed BLAST, GO enrichment, and KEGG pathway analyses on the up- and downregulated proteins to elucidate correlated events in the C. suis life cycle. Our analyses revealed intriguing metabolic patterns in macromolecule metabolism, DNA- and RNA-related processes, proteins associated with sexual stages, and those involved in cell invasion, reflecting the adaptation of sexual stages to a nutrient-poor and potentially stressful extracellular environment, with a focus on enzymes involved in metabolism and energy production.\nDiscussion: These findings have important implications for understanding the developmental biology of C. suis as well as other, related coccidian parasites, such as Eimeria spp. and Toxoplasma gondii. They also support the role of C. suis as a new model for the comparative biology of coccidian tissue cyst stages.", "claims": [{"id": "claim-method", "statement": "Differential protein expression analysis was performed with function dream in package variancePartition version 1.28.0 (Hoffman and Roussos, 2021), on log2-transformed, normalized LC-MS/MS quantifications after adding a constant of 1 via univariate linear mixed-effect model fitting timepoint of development as a fixed categorical effect with factor levels day 7, 8, 10, 12, and 14.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37953800", "locator": "sections[9] \"Identification and analysis of differentially expressed proteins\" paragraph 4", "quote": "Differential protein expression analysis was performed with function dream in package variancePartition version 1.28.0 (Hoffman and Roussos, 2021), on log2-transformed, normalized LC-MS/MS quantifications after adding a constant of 1 via univariate linear mixed-effect model fitting timepoint of development as a fixed categorical effect with factor levels day 7, 8, 10, 12, and 14."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets presented in this study can be found in online repositories. 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The names of the repository/repositories and accession number(s) can be found below: ProteomeXchange with identifier PXD045050."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37953800", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A total of 1,324 proteins were identified in the in vitro developmental stages of C. suis, and 1,082 proteins were identified as significantly differentially expressed.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37953800"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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