Structural Similarities, in Relation with the Cross-Reactivity, of Hymenoptera Allergenic Dipeptidyl Peptidases IV-An Overall Comparison Including a New Dipeptidyl Peptidase IV Sequence from Vespa velutina.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:37999519", "kind": "science-record", "revision": 2, "content_hash": "sha256:6fa6945bc2747a3f0d4e9a70dba4065ec467adf565a58eb3621a459f84bf5a10", "created_at": "2026-11-01T19:02:55Z", "updated_at": "2026-11-01T19:19:55Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Structural Similarities, in Relation with the Cross-Reactivity, of Hymenoptera Allergenic Dipeptidyl Peptidases IV-An Overall Comparison Including a New Dipeptidyl Peptidase IV Sequence from Vespa velutina.", "doi": "10.3390/toxins15110656", "pmid": "37999519", "authors": ["Rafael I Monsalve", "Manuel Lombardero", "Lars H Christensen", "Beatriz Núñez-Acevedo", "David González-de-Olano", "Miriam Sobrino-García", "Rosita M Castillo-Loja", "Susana B Bravo", "Manuela Alonso-Sampedro", "Carmen Vidal"], "journal": "Toxins", "year": 2023, "abstract": "(1) Background: Dipeptidyl Peptidases IV (DPPIVs), present in many organisms, are minor components in the venoms of Hymenoptera, where they have been identified as cross-reactive allergenic molecules. Considering that the structure of homologous DPPIVs is well characterized, we aimed to explain which regions have higher similarity among these proteins and present a comparison among them, including a new Vespa velutina DPPIV sequence. Moreover, two cases of sensitization to DPPIVs in wasp- and honeybee-sensitized patients are presented. (2) Methods: Proteomic analyses have been performed on the venom of the Asian hornet Vespa velutina to demonstrate the sequence of its DPPIV (allergen named Vesp v 3, with sequence accession number P0DRB8, and with the proteomic data available via ProteomeXchange with the identifier PXD046030). A comparison performed through their alignments and analysis of the three-dimensional structure showed a region with higher similarity among Hymenoptera DPPIVs. Additionally, ImmunoCAP™ determinations (including specific inhibition experiments), as well as IgE immunoblotting, are performed to demonstrate the allergenicity of Api m 5 and Ves v 3. (3) Results and Conclusions: The data presented demonstrate that the similarities among Hymenoptera DPPIVs are most likely localized at the C-terminal region of these enzymes. In addition, a higher similarity of the Vespa/Vespula DPPIVs is shown. The clinical cases analyzed demonstrated the allergenicity of Api m 5 and Ves v 3 in the sera of the allergic patients, as well as the presence of this minor component in the preparations used in venom immunotherapy.", "claims": [{"id": "claim-data-access", "statement": "The data presented in this work were made public by the authors by explicitly making public the new Vespa velutina sequence (UniProtKB database accession number P0DRB8), publishing the raw data in the Supplementary Material files and depositing the proteomic data generated into the PRIDE partner repository with the dataset identifier PXD046030.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:37999519", "locator": "sections[22] \"Data Availability Statement\" paragraph 2", "quote": "The data presented in this work were made public by the authors by explicitly making public the new Vespa velutina sequence (UniProtKB database accession number P0DRB8), publishing the raw data in the Supplementary Material files and depositing the proteomic data generated into the PRIDE partner repository with the dataset identifier PXD046030."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The data presented demonstrate that the similarities among Hymenoptera DPPIVs are most likely localized at the C-terminal region of these enzymes.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:37999519", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "The data presented demonstrate that the similarities among Hymenoptera DPPIVs are most likely localized at the C-terminal region of these enzymes."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The data presented demonstrate that the similarities among Hymenoptera DPPIVs are most likely localized at the C-terminal region of these enzymes.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:37999519"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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